| LEESCH, Friederike, Laura LORENZO-ORTS, Carina PRIBITZER, Irina GRISHKOVSKAYA, Josef ROEHSNER, Anastasia CHUGUNOVA, Manuel MATZINGER, Elisabeth ROITINGER, Katarina BELACIC, Susanne KANDOLF, Tzi-Yang LIN, Karl MECHTLER, Anton MEINHART, David HASELBACH a Andrea PAULI. A molecular network of conserved factors keeps ribosomes dormant in the egg. Nature. BERLIN: NATURE PORTFOLIO, 2023, roč. 613, č. 7945, s. 712-736. ISSN 0028-0836. Dostupné z: https://dx.doi.org/10.1038/s41586-022-05623-y. |
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@article{2383605,
author = {Leesch, Friederike and LorenzoandOrts, Laura and Pribitzer, Carina and Grishkovskaya, Irina and Roehsner, Josef and Chugunova, Anastasia and Matzinger, Manuel and Roitinger, Elisabeth and Belacic, Katarina and Kandolf, Susanne and Lin, TziandYang and Mechtler, Karl and Meinhart, Anton and Haselbach, David and Pauli, Andrea},
article_location = {BERLIN},
article_number = {7945},
doi = {http://dx.doi.org/10.1038/s41586-022-05623-y},
keywords = {CRYO-EM; STRUCTURES; SEA-URCHIN; EGGS; PROTEIN-SYNTHESIS; ANTIMICROBIAL PEPTIDE; PROMOTES TRANSLATION; CELL-PROLIFERATION; PROFILING REVEALS; MESSENGER-RNA; VISUALIZATION},
language = {eng},
issn = {0028-0836},
journal = {Nature},
title = {A molecular network of conserved factors keeps ribosomes dormant in the egg},
url = {https://www.nature.com/articles/s41586-022-05623-y},
volume = {613},
year = {2023}
}
TY - JOUR
ID - 2383605
AU - Leesch, Friederike - Lorenzo-Orts, Laura - Pribitzer, Carina - Grishkovskaya, Irina - Roehsner, Josef - Chugunova, Anastasia - Matzinger, Manuel - Roitinger, Elisabeth - Belacic, Katarina - Kandolf, Susanne - Lin, Tzi-Yang - Mechtler, Karl - Meinhart, Anton - Haselbach, David - Pauli, Andrea
PY - 2023
TI - A molecular network of conserved factors keeps ribosomes dormant in the egg
JF - Nature
VL - 613
IS - 7945
SP - 712-736
EP - 712-736
PB - NATURE PORTFOLIO
SN - 00280836
KW - CRYO-EM
KW - STRUCTURES
KW - SEA-URCHIN
KW - EGGS
KW - PROTEIN-SYNTHESIS
KW - ANTIMICROBIAL PEPTIDE
KW - PROMOTES TRANSLATION
KW - CELL-PROLIFERATION
KW - PROFILING REVEALS
KW - MESSENGER-RNA
KW - VISUALIZATION
UR - https://www.nature.com/articles/s41586-022-05623-y
N2 - Ribosomes are produced in large quantities during oogenesis and are stored in the egg. However, the egg and early embryo are translationally repressed(1-4). Here, using mass spectrometry and cryo-electron microscopy analyses of ribosomes isolated from zebrafish (Danio rerio) and Xenopus laevis eggs and embryos, we provide molecular evidence that ribosomes transition from a dormant state to an active state during the first hours of embryogenesis. Dormant ribosomes are associated with four conserved factors that form two modules, consisting of Habp4-eEF2 and death associated protein 1b (Dap1b) or Dap in complex with eIF5a. Both modules occupy functionally important sites and act together to stabilize ribosomes and repress translation. Dap1b (also known as Dapl1 in mammals) is a newly discovered translational inhibitor that stably inserts into the polypeptide exit tunnel. Addition of recombinant zebrafish Dap1b protein is sufficient to block translation and reconstitute the dormant egg ribosome state in a mammalian translation extract in vitro. Thus, a developmentally programmed, conserved ribosome state has a key role in ribosome storage and translational repression in the egg.
ER -
LEESCH, Friederike, Laura LORENZO-ORTS, Carina PRIBITZER, Irina GRISHKOVSKAYA, Josef ROEHSNER, Anastasia CHUGUNOVA, Manuel MATZINGER, Elisabeth ROITINGER, Katarina BELACIC, Susanne KANDOLF, Tzi-Yang LIN, Karl MECHTLER, Anton MEINHART, David HASELBACH a Andrea PAULI. A molecular network of conserved factors keeps ribosomes dormant in the egg. \textit{Nature}. BERLIN: NATURE PORTFOLIO, 2023, roč.~613, č.~7945, s.~712-736. ISSN~0028-0836. Dostupné z: https://dx.doi.org/10.1038/s41586-022-05623-y.
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