ELEMENTS AND PROTEINS IMAGING BY LA-ICP-MS: MEDICAL AND
PHARMACEUTICAL RESEARCH

p 2023

ELEMENTS AND PROTEINS IMAGING BY LA-ICP-MS: MEDICAL AND PHARMACEUTICAL RESEARCH

KUCHYNKA, Michaela, Marcela VLČNOVSKÁ, Kim KALLALA, Jan BISKUPIČ, Jana HLOŽKOVÁ et. al.

Základní údaje

Originální název

ELEMENTS AND PROTEINS IMAGING BY LA-ICP-MS: MEDICAL AND PHARMACEUTICAL RESEARCH

Název česky

Zobrazování prvků a proteinů pomocí metody LA-ICP-MS: medicínský a farmaceutický výzkum

Autoři

KUCHYNKA, Michaela (203 Česká republika, garant, domácí), Marcela VLČNOVSKÁ (203 Česká republika, domácí), Kim KALLALA (203 Česká republika, domácí), Jan BISKUPIČ (203 Česká republika, domácí), Jana HLOŽKOVÁ (203 Česká republika, domácí), Peter SCHEER (203 Česká republika, domácí), Tereza PADRTOVÁ (203 Česká republika, domácí), Radka OPATŘILOVÁ (203 Česká republika, domácí), Markéta VACULOVIČOVÁ (203 Česká republika), Viktor KANICKÝ (203 Česká republika, domácí), Michal MASAŘÍK (203 Česká republika, domácí) a Tomáš VACULOVIČ (203 Česká republika, domácí)

Vydání

Vyzvaná přednáška na výzkumném institutu - Vall d'Hebron Talks by VHIR, 2023

Další údaje

Jazyk

angličtina

Typ výsledku

Vyžádané přednášky

Obor

10406 Analytical chemistry

Stát vydavatele

Španělsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

Vall d'Hebron Talks by VHIR 'Elements and proteins imaging by LA-ICP-MS: medical and pharmaceutical research'

Kód RIV

RIV/00216224:14160/23:00133769

Organizační jednotka

Farmaceutická fakulta

Klíčová slova česky

biologické zobrazování; laserová ablace; detekce proteinu; prvková distribuce

Klíčová slova anglicky

Bioimaging; laser ablation; protein detection; element distribution

Štítky

PATFYZ, ÚCh, ÚChL, ÚFTo

Příznaky

Mezinárodní význam

Změněno: 13. 3. 2024 10:54, Mgr. Michaela Kuchynka, Ph.D.

Anotace

V originále

In the last decades, increased interest in imaging of elements/metals/proteins distribution, mainly in pharmaceutical, biomedicine research, or life science with direct application of research to specific problem/disease [1-3]. The main emphasis of this contribution is to demonstrate the unique method of laser ablation with inductively coupled plasma and mass spectrometry (LA-ICP-MS). It is a well-established method for multi-elemental analysis of elements at trace and ultra-trace. Nowadays, it is also starting to be used as a technique for the simultaneous specific determination of the protein of interest, which opens up the possibility of achieving the so-called multiplex analysis. Protein determination, protein imaging and finally their distribution in clinical samples are presented. Proteins are imaged using an immunohistochemical method (binding nanoparticle-labeled antibodies to a specific protein) and LA-ICP-MS. The main advantages of utilizing LA-ICP-MS are the acquisition of comprehensive (Metallo)proteomic information about the tissue of a given disease (in this case, cancer or stroke) or low detection limits compared to other conventionally employed protein imaging techniques in combination with multiplex analysis of one sample.

Návaznosti

MUNI/R/1568/2020, interní kód MU

Název: Study the effectiveness of anticancer drugs (Akronym: STEAD)

Investor: Masarykova univerzita, Study the effectiveness of anticancer drugs, CAREER RESTART

Citovat

KUCHYNKA, Michaela, Marcela VLČNOVSKÁ, Kim KALLALA, Jan BISKUPIČ, Jana HLOŽKOVÁ, Peter SCHEER, Tereza PADRTOVÁ, Radka OPATŘILOVÁ, Markéta VACULOVIČOVÁ, Viktor KANICKÝ, Michal MASAŘÍK a Tomáš VACULOVIČ. ELEMENTS AND PROTEINS IMAGING BY LA-ICP-MS: MEDICAL AND PHARMACEUTICAL RESEARCH. In Vyzvaná přednáška na výzkumném institutu - Vall d'Hebron Talks by VHIR. 2023.

@misc{2383639,
   author = {Kuchynka, Michaela and Vlčnovská, Marcela and Kallala, Kim and Biskupič, Jan and Hložková, Jana and Scheer, Peter and Padrtová, Tereza and Opatřilová, Radka and Vaculovičová, Markéta and Kanický, Viktor and Masařík, Michal and Vaculovič, Tomáš},
   booktitle = {Vyzvaná přednáška na výzkumném institutu - Vall d'Hebron Talks by VHIR},
   keywords = {Bioimaging; laser ablation; protein detection; element distribution},
   language = {eng},
   title = {ELEMENTS AND PROTEINS IMAGING BY LA-ICP-MS: MEDICAL AND PHARMACEUTICAL RESEARCH},
   url = {https://www.vallhebron.com/actualitat/agenda/vall-dhebron-talks-vhir-elements-and-proteins-imaging-la-icp-ms-medical-and-pharmaceutical-research},
   year = {2023}
}

TY  - SLIDE
ID  - 2383639
AU  - Kuchynka, Michaela - Vlčnovská, Marcela - Kallala, Kim - Biskupič, Jan - Hložková, Jana - Scheer, Peter - Padrtová, Tereza - Opatřilová, Radka - Vaculovičová, Markéta - Kanický, Viktor - Masařík, Michal - Vaculovič, Tomáš
PY  - 2023
TI  - ELEMENTS AND PROTEINS IMAGING BY LA-ICP-MS: MEDICAL AND PHARMACEUTICAL RESEARCH
KW  - Bioimaging
KW  - laser ablation
KW  - protein detection
KW  - element distribution
UR  - https://www.vallhebron.com/actualitat/agenda/vall-dhebron-talks-vhir-elements-and-proteins-imaging-la-icp-ms-medical-and-pharmaceutical-research
N2  - In the last decades, increased interest in imaging of elements/metals/proteins distribution, mainly in pharmaceutical, biomedicine research, or life science with direct application of research to specific problem/disease [1-3]. The main emphasis of this contribution is to demonstrate the unique method of laser ablation with inductively coupled plasma and mass spectrometry (LA-ICP-MS). It is a well-established method for multi-elemental analysis of elements at trace and ultra-trace. Nowadays, it is also starting to be used as a technique for the simultaneous specific determination of the protein of interest, which opens up the possibility of achieving the so-called multiplex analysis. Protein determination, protein imaging and finally their distribution in clinical samples are presented. Proteins are imaged using an immunohistochemical method (binding nanoparticle-labeled antibodies to a specific protein) and LA-ICP-MS. The main advantages of utilizing LA-ICP-MS are the acquisition of comprehensive (Metallo)proteomic information about the tissue of a given disease (in this case, cancer or stroke) or low detection limits compared to other conventionally employed protein imaging techniques in combination with multiplex analysis of one sample.
ER  -

KUCHYNKA, Michaela, Marcela VLČNOVSKÁ, Kim KALLALA, Jan BISKUPIČ, Jana HLOŽKOVÁ, Peter SCHEER, Tereza PADRTOVÁ, Radka OPATŘILOVÁ, Markéta VACULOVIČOVÁ, Viktor KANICKÝ, Michal MASAŘÍK a Tomáš VACULOVIČ. ELEMENTS AND PROTEINS IMAGING BY LA-ICP-MS: MEDICAL AND PHARMACEUTICAL RESEARCH. In \textit{Vyzvaná přednáška na výzkumném institutu - Vall d'Hebron Talks by VHIR}. 2023.

Podrobný výpis o publikaci