J 2023

The Gold(I) Complex with Plant Hormone Kinetin Shows Promising In Vitro Anticancer and PPARγ Properties

TRAVNICEK, Zdenek, Jan VANCO, Jan BELZA, Jan HOSEK, Zdenek DVORAK et. al.

Základní údaje

Originální název

The Gold(I) Complex with Plant Hormone Kinetin Shows Promising In Vitro Anticancer and PPARγ Properties

Autoři

TRAVNICEK, Zdenek (203 Česká republika), Jan VANCO (203 Česká republika), Jan BELZA (203 Česká republika), Jan HOSEK (203 Česká republika), Zdenek DVORAK (203 Česká republika), Rene LENOBEL (203 Česká republika), Igor POPA (203 Česká republika), Karel ŠMEJKAL (203 Česká republika, domácí) a Pavel UHRIN (40 Rakousko)

Vydání

International Journal of Molecular Sciences, Basel, Multidisciplinary Digital Publishing Institute, 2023, 1661-6596

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30104 Pharmacology and pharmacy

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

Impakt faktor

Impact factor: 5.600 v roce 2022

Kód RIV

RIV/00216224:14160/23:00133785

Organizační jednotka

Farmaceutická fakulta

UT WoS

000930771000001

Klíčová slova anglicky

gold(I) complex; kinetin; anticancer; anti-inflammatory; in vitro; PPAR; cell cycle; apoptosis; ROS

Štítky

Příznaky

Mezinárodní význam, Recenzováno
Změněno: 4. 4. 2024 19:10, Mgr. Daniela Černá

Anotace

V originále

Motivated by the clinical success of gold(I) metallotherapeutic Auranofin in the effective treatment of both inflammatory and cancer diseases, we decided to prepare, characterize, and further study the [Au(kin)(PPh3)] complex (1), where Hkin = kinetin, 6-furfuryladenine, for its in vitro anti-cancer and anti-inflammatory activities. The results revealed that the complex (1) had significant in vitro cytotoxicity against human cancer cell lines (A2780, A2780R, PC-3, 22Rv1, and THP-1), with IC50 approximate to 1-5 mu M, which was even significantly better than that for the conventional platinum-based drug Cisplatin while comparable with Auranofin. Although its ability to inhibit transcription factor NF-kappa B activity did not exceed the comparative drug Auranofin, it has been found that it is able to positively influence peroxisome-proliferator-activated receptor-gamma (PPAR gamma), and as a consequence of this to have the impact of moderating/reducing inflammation. The cellular effects of the complex (1) in A2780 cancer cells were also investigated by cell cycle analysis, induction of apoptosis, intracellular ROS production, activation of caspases 3/7 and disruption of mitochondrial membrane potential, and shotgun proteomic analysis. Proteomic analysis of R2780 cells treated with complex (1) and starting compounds revealed possible different places of the effect of the studied compounds. Moreover, the time-dependent cellular accumulation of copper was studied by means of the mass spectrometry study with the aim of exploring the possible mechanisms responsible for its biological effects.

Návaznosti

GF21-38204L, projekt VaV
Název: Komplexy vybraných přechodných kovů s rostlinnými látkami s anti-NF-kappa B a pro-PPAR duální aktivitou
Investor: Grantová agentura ČR, Komplexy vybraných přechodných kovů s rostlinnými látkami s anti-NF-kappa B a pro-PPAR duální aktivitou, Partnerská agentura (Rakousko)