J 2024

Long-term dietary exposure to the non-steroidal anti-inflammatory drugs diclofenac and ibuprofen can affect the physiology of common carp (<i>Cyprinus carpio</i>) on multiple levels, even at "environmentally relevant" concentrations

MIKULA, Premysl, Aneta HOLLEROVA, Nikola HODKOVICOVA, Veronika DOUBKOVA, Petr MARSALEK et. al.

Základní údaje

Originální název

Long-term dietary exposure to the non-steroidal anti-inflammatory drugs diclofenac and ibuprofen can affect the physiology of common carp (<i>Cyprinus carpio</i>) on multiple levels, even at "environmentally relevant" concentrations

Autoři

MIKULA, Premysl, Aneta HOLLEROVA (203 Česká republika), Nikola HODKOVICOVA (203 Česká republika), Veronika DOUBKOVA (203 Česká republika), Petr MARSALEK (203 Česká republika), Aleš FRANC (203 Česká republika, domácí), Lucie SEDLÁČKOVÁ (203 Česká republika, domácí), Renata HESOVA (203 Česká republika), Helena MODRA (203 Česká republika), Zdenka SVOBODOVA (203 Česká republika) a Jana BLAHOVA (203 Česká republika)

Vydání

Science of the Total Environment, AMSTERDAM, Elsevier, 2024, 0048-9697

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30104 Pharmacology and pharmacy

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

Impakt faktor

Impact factor: 9.800 v roce 2022

Organizační jednotka

Farmaceutická fakulta

DOI

UT WoS

001178917700001

Klíčová slova anglicky

Fish; Haematological indices; Oxidative stress; Plasma biochemistry; Gene expression; Thyroid disruption

Štítky

Příznaky

Mezinárodní význam, Recenzováno
Změněno: 20. 3. 2024 12:33, Mgr. Daniela Černá

Anotace

V originále

The aim of the study was to evaluate the effects of emerging environmental contaminants, the non-steroidal anti-inflammatory drugs (NSAIDs) diclofenac (DCF) and ibuprofen (IBP), on physiological functions in juvenile common carp (Cyprinus carpio). Fish were exposed for 6 weeks, and for the first time, NSAIDs were administered through diet. Either substance was tested at two concentrations, 20 or 2000 mu g/kg, resulting in four different treatments (DCF 20, DCF 2000, IBP 20, IBP 2000). The effects on haematological and biochemical profiles, the biomarkers of oxidative stress, and endocrine disruption were studied, and changes in RNA transcription were also monitored to obtain a comprehensive picture of toxicity. Fish exposure to high concentrations of NSAIDs (DCF 2000, IBP 2000) elicited numerous statistically significant changes (p < 0.05) in the endpoints investigated, with DCF being almost always more efficient than IBP. Compared to control fish, a decrease in total leukocyte count attributed to relative lymphopenia was observed. Plasma concentrations of total proteins, ammonia, and thyroxine, and enzyme activities of alanine aminotransferase (ALT), aspartate aminotransferase, and alkaline phosphatase (ALP) were significantly elevated in either group, as were the activities of certain hepatic antioxidant enzymes (superoxide dismutase, glutathione-S-transferase) in the DCF 2000 group. The transcriptomic profile of selected genes in the tissues of exposed fish was affected as well. Significant changes in plasma total proteins, ammonia, ALT, and ALP, as well as in the transcription of genes related to thyroid function and the antioxidant defense of the organism, were found even in fish exposed to the lower DCF concentration (DCF 20). As it was chosen to match DCF concentrations commonly detected in aquatic invertebrates (i.e., the potential feed source of fish), it can be considered "environmentally relevant". Future research is necessary to shed more light on the dietary NSAID toxicity to fish.