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@article{2386417,
author = {Bozděchová, Lucie and Rudolfová, Anna and Hanáková, Kateřina and Fojtová, Miloslava and Fajkus, Jiří},
article_number = {6},
doi = {http://dx.doi.org/10.3390/plants13060850},
keywords = {Arabidopsis thaliana; ChIRP-MS; RNA; RNA-centric methods; RNA-protein interactions; telomerase; telomerase RNA},
language = {eng},
issn = {2223-7747},
journal = {Plants},
title = {Optimizing ChIRP-MS for Comprehensive Profiling of RNA-Protein Interactions in Arabidopsis thaliana: A Telomerase RNA Case Study},
url = {https://www.mdpi.com/2223-7747/13/6/850},
volume = {13},
year = {2024}
}
TY - JOUR
ID - 2386417
AU - Bozděchová, Lucie - Rudolfová, Anna - Hanáková, Kateřina - Fojtová, Miloslava - Fajkus, Jiří
PY - 2024
TI - Optimizing ChIRP-MS for Comprehensive Profiling of RNA-Protein Interactions in Arabidopsis thaliana: A Telomerase RNA Case Study
JF - Plants
VL - 13
IS - 6
SP - 1-12
EP - 1-12
PB - MDPI
SN - 22237747
KW - Arabidopsis thaliana
KW - ChIRP-MS
KW - RNA
KW - RNA-centric methods
KW - RNA-protein interactions
KW - telomerase
KW - telomerase RNA
UR - https://www.mdpi.com/2223-7747/13/6/850
N2 - The current repertoire of methods available for studying RNA-protein interactions in plants is somewhat limited. Employing an RNA-centric approach, particularly with less abundant RNAs, presents various challenges. Many of the existing methods were initially designed for different model systems, with their application in plants receiving limited attention thus far. The Comprehensive Identification of RNA-Binding Proteins by Mass Spectrometry (ChIRP-MS) technique, initially developed for mammalian cells, has been adapted in this study for application in Arabidopsis thaliana. The procedures have been meticulously modified and optimized for telomerase RNA, a notable example of a low-abundance RNA recently identified. Following these optimization steps, ChIRP-MS can serve as an effective screening method for identifying candidate proteins interacting with any target RNA of interest.
ER -
BOZDĚCHOVÁ, Lucie, Anna RUDOLFOVÁ, Kateřina HANÁKOVÁ, Miloslava FOJTOVÁ a Jiří FAJKUS. Optimizing ChIRP-MS for Comprehensive Profiling of RNA-Protein Interactions in Arabidopsis thaliana: A Telomerase RNA Case Study. \textit{Plants}. MDPI, 2024, roč.~13, č.~6, s.~1-12. ISSN~2223-7747. Dostupné z: https://dx.doi.org/10.3390/plants13060850.
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