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@article{2388197,
author = {Juráček, Jaroslav and Boudná, Marie and Staník, Michal and Ručková, Michaela and Trachtová, Karolína and Malčíková, Hana and Lžičařová, Eva and Barth, Dominik A and Pichler, Martin and Slabý, Ondřej},
article_location = {HOBOKEN},
article_number = {4},
doi = {http://dx.doi.org/10.1002/pros.24466},
keywords = {disease aggressiveness; microRNA; prognosis; prostate cancer; prostate specific antigen},
language = {eng},
issn = {0270-4137},
journal = {Prostate},
title = {A tissue miRNA expression pattern is associated with disease aggressiveness of localized prostate cancer},
url = {https://onlinelibrary.wiley.com/doi/10.1002/pros.24466},
volume = {83},
year = {2023}
}
TY - JOUR
ID - 2388197
AU - Juráček, Jaroslav - Boudná, Marie - Staník, Michal - Ručková, Michaela - Trachtová, Karolína - Malčíková, Hana - Lžičařová, Eva - Barth, Dominik A - Pichler, Martin - Slabý, Ondřej
PY - 2023
TI - A tissue miRNA expression pattern is associated with disease aggressiveness of localized prostate cancer
JF - Prostate
VL - 83
IS - 4
SP - 340-351
EP - 340-351
PB - WILEY
SN - 02704137
KW - disease aggressiveness
KW - microRNA
KW - prognosis
KW - prostate cancer
KW - prostate specific antigen
UR - https://onlinelibrary.wiley.com/doi/10.1002/pros.24466
N2 - BackgroundProstate cancer (PCa) is a heterogeneous malignancy with high variability in clinical course. Insufficient stratification according to the aggressiveness at the time of diagnosis causes unnecessary or delayed treatment. Current stratification systems are not effective enough because they are based on clinical, surgical or biochemical parameters, but do not take into account molecular factors driving PCa cancerogenesis. MicroRNAs (miRNAs) are important players in molecular pathogenesis of PCa and could serve as valuable biomarkers for the assessment of disease aggressiveness and its prognosis. MethodsIn the study, in total, 280 PCa patients were enrolled. The miRNA expression profiles were analyzed in FFPE PCa tissue using the miRCURY LNA miRNA PCR System. The expression levels of candidate miRNAs were further verified by two-level validation using the RT-qPCR method and evaluated in relation to PCa stratification reflecting the disease aggressiveness. ResultsMiRNA profiling revealed 172 miRNAs dysregulated between aggressive (ISUP 3-5) and indolent PCa (ISUP 1) (p < 0.05). In the training and validation cohort, miR-15b-5p and miR-106b-5p were confirmed to be significantly upregulated in tissue of aggressive PCa when their level was associated with disease aggressiveness. Furthermore, we established a prognostic score combining the level of miR-15b-5p and miR-106b-5p with serum PSA level, which discriminated indolent PCa from an aggressive form with even higher analytical parameters (AUC being 0.9338 in the training set and 0.8014 in the validation set, respectively). The score was also associated with 5-year biochemical progression-free survival (bPFS) of PCa patients. ConclusionsWe identified a miRNA expression pattern associated with disease aggressiveness in prostate cancer patients. These miRNAs may be of biological interest as the focus can be also set on their specific role within the molecular pathology and the molecular mechanism that underlies the aggressivity of prostate cancer.
ER -
JURÁČEK, Jaroslav, Marie BOUDNÁ, Michal STANÍK, Michaela RUČKOVÁ, Karolína TRACHTOVÁ, Hana MALČÍKOVÁ, Eva LŽIČAŘOVÁ, Dominik A BARTH, Martin PICHLER a Ondřej SLABÝ. A tissue miRNA expression pattern is associated with disease aggressiveness of localized prostate cancer. \textit{Prostate}. HOBOKEN: WILEY, 2023, roč.~83, č.~4, s.~340-351. ISSN~0270-4137. Dostupné z: https://dx.doi.org/10.1002/pros.24466.
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