Photoelectrochemical Enzyme Biosensor for Malate Using Quantum
Dots on Indium Tin Oxide/Plastics as a Sensing Surface

J 2024

Photoelectrochemical Enzyme Biosensor for Malate Using Quantum Dots on Indium Tin Oxide/Plastics as a Sensing Surface

HLAVÁČOVÁ, Tereza a Petr SKLÁDAL

Základní údaje

Originální název

Photoelectrochemical Enzyme Biosensor for Malate Using Quantum Dots on Indium Tin Oxide/Plastics as a Sensing Surface

Autoři

HLAVÁČOVÁ, Tereza (203 Česká republika, domácí) a Petr SKLÁDAL (203 Česká republika, garant, domácí)

Vydání

Biosensors, MDPI, 2024, 2079-6374

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 5.400 v roce 2022

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.3390/bios14010011

UT WoS

001149456100001

Klíčová slova anglicky

malate dehydrogenase; flow-through biosensor; electrochemical sensor; wine; fruit juice

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 11. 4. 2024 10:45, Mgr. Marie Šípková, DiS.

Anotace

V originále

A photoelectrochemical biosensor for malate was developed using an indium tin oxide (ITO) layer deposited on a poly(ethylene terephthalate) plastic sheet as a transparent electrode material for the immobilization of malate dehydrogenase together with CdTe quantum dots. Different approaches were compared for the construction of the bioactive layer; the highest response was achieved by depositing malate dehydrogenase together with CdTe nanoparticles and covering it with a Nafion/water (1:1) mixture. The amperometric signal of this biosensor was recorded during irradiation with a near-UV LED in the flow-through mode. The limit of detection was 0.28 mmol/L, which is adequate for analyzing malic acid levels in drinks such as white wines and fruit juices. The results confirm that the cheap ITO layer deposited on the plastic sheet after cutting into rectangular electrodes allows for the economic production of photoelectrochemical (bio)sensors. The combination of NAD+-dependent malate dehydrogenase with quantum dots was also compatible with such an ITO surface.

Návaznosti

MUNI/A/1313/2022, interní kód MU

Název: Podpora biochemického výzkumu v roce 2023

Investor: Masarykova univerzita, Podpora biochemického výzkumu v roce 2023

Citovat

HLAVÁČOVÁ, Tereza a Petr SKLÁDAL. Photoelectrochemical Enzyme Biosensor for Malate Using Quantum Dots on Indium Tin Oxide/Plastics as a Sensing Surface. Biosensors. MDPI, 2024, roč. 14, č. 1, s. 1-13. ISSN 2079-6374. Dostupné z: https://dx.doi.org/10.3390/bios14010011.

@article{2393097,
   author = {Hlaváčová, Tereza and Skládal, Petr},
   article_number = {1},
   doi = {http://dx.doi.org/10.3390/bios14010011},
   keywords = {malate dehydrogenase; flow-through biosensor; electrochemical sensor; wine; fruit juice},
   language = {eng},
   issn = {2079-6374},
   journal = {Biosensors},
   title = {Photoelectrochemical Enzyme Biosensor for Malate Using Quantum Dots on Indium Tin Oxide/Plastics as a Sensing Surface},
   url = {https://www.mdpi.com/2079-6374/14/1/11},
   volume = {14},
   year = {2024}
}

TY  - JOUR
ID  - 2393097
AU  - Hlaváčová, Tereza - Skládal, Petr
PY  - 2024
TI  - Photoelectrochemical Enzyme Biosensor for Malate Using Quantum Dots on Indium Tin Oxide/Plastics as a Sensing Surface
JF  - Biosensors
VL  - 14
IS  - 1
SP  - 1-13
EP  - 1-13
PB  - MDPI
SN  - 20796374
KW  - malate dehydrogenase
KW  - flow-through biosensor
KW  - electrochemical sensor
KW  - wine
KW  - fruit juice
UR  - https://www.mdpi.com/2079-6374/14/1/11
N2  - A photoelectrochemical biosensor for malate was developed using an indium tin oxide (ITO) layer deposited on a poly(ethylene terephthalate) plastic sheet as a transparent electrode material for the immobilization of malate dehydrogenase together with CdTe quantum dots. Different approaches were compared for the construction of the bioactive layer; the highest response was achieved by depositing malate dehydrogenase together with CdTe nanoparticles and covering it with a Nafion/water (1:1) mixture. The amperometric signal of this biosensor was recorded during irradiation with a near-UV LED in the flow-through mode. The limit of detection was 0.28 mmol/L, which is adequate for analyzing malic acid levels in drinks such as white wines and fruit juices. The results confirm that the cheap ITO layer deposited on the plastic sheet after cutting into rectangular electrodes allows for the economic production of photoelectrochemical (bio)sensors. The combination of NAD+-dependent malate dehydrogenase with quantum dots was also compatible with such an ITO surface.
ER  -

HLAVÁČOVÁ, Tereza a Petr SKLÁDAL. Photoelectrochemical Enzyme Biosensor for Malate Using Quantum Dots on Indium Tin Oxide/Plastics as a Sensing Surface. \textit{Biosensors}. MDPI, 2024, roč.~14, č.~1, s.~1-13. ISSN~2079-6374. Dostupné z: https://dx.doi.org/10.3390/bios14010011.

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