Utility of RNA sequencing for transcriptome analysis of small
extracellular vesicles derived from blood sera of colorectal
cancer patients

a 2023

Utility of RNA sequencing for transcriptome analysis of small extracellular vesicles derived from blood sera of colorectal cancer patients

MACHÁČKOVÁ, Táňa, Petra VYCHYTILOVÁ, Marie BOUDNÁ, Karolína TRACHTOVÁ, Marketa PAVLIKOVA et. al.

Základní údaje

Originální název

Utility of RNA sequencing for transcriptome analysis of small extracellular vesicles derived from blood sera of colorectal cancer patients

Autoři

MACHÁČKOVÁ, Táňa (203 Česká republika, domácí), Petra VYCHYTILOVÁ (203 Česká republika, domácí), Marie BOUDNÁ (203 Česká republika, domácí), Karolína TRACHTOVÁ (203 Česká republika, domácí), Marketa PAVLIKOVA, Jan KOTOUCEK, Jana HALÁMKOVÁ (203 Česká republika, domácí), Dagmar AL TUKMACHI (203 Česká republika, domácí), Jiří ŠÁNA (203 Česká republika, domácí), Petra POKORNÁ (203 Česká republika, domácí), Milana ŠACHLOVÁ (203 Česká republika, domácí) a Ondřej SLABÝ (203 Česká republika, garant, domácí)

Vydání

114th Annual Meeting of the American Association for Cancer Research (AACR), 2023

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

30204 Oncology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Kód RIV

RIV/00216224:14740/23:00133957

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1158/1538-7445.AM2023-6709

UT WoS

001008499107019

Klíčová slova anglicky

colorectal cancer; biomarkers; non-coding RNAs; micro-RNA; small extracellular vesicles

Štítky

CF CIISB, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 28. 8. 2024 22:47, Mgr. Eva Dubská

Anotace

V originále

Currently used molecular diagnostic tests for colorectal cancer (CRC) are underperforming and more sensitive, non-invasive biomarkers are needed. Long non-coding RNAs (lncRNA) and microRNA (miRNA) have shown potential as diagnostic biomarkers. Unfortunately, the identification of non-coding RNA circulating biomarkers in blood serum is significantly burdened by abundant RNA specimens from disrupted blood cells. Recently, small extracellular vesicles (sEVs) emerged as potential reservoirs of clinically relevant biomarkers, including lncRNAs and miRNAs. In theory, sEVs protect RNAs from degradation and might serve as a source of intact RNA for further analyses. However, there is a lack of evidence supporting the superior quality of RNA extracted from sEVs over RNA from whole blood serum. This study aimed to analyze the RNA content of blood serum and the sEVs derived from the blood serum of CRC patients and healthy controls using RNA sequencing. Moreover, small RNA sequencing was used to evaluate the difference in miRNA profiles of sEVs and corresponding blood sera of CRC patients and healthy controls.

Návaznosti

LM2018127, projekt VaV

Název: Česká infrastruktura pro integrativní strukturní biologii (Akronym: CIISB)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Czech Infrastructure for Integrative Structural Biology

LX22NPO5102, projekt VaV

Název: Národní ústav pro výzkum rakoviny (Akronym: NÚVR)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Národní ústav pro výzkum rakoviny, 5.1 EXCELES

NU20-03-00127, projekt VaV

Název: Dlouhé nekódující RNA v exozómech jako biomarkery umožňující neinvazivní časnou detekci kolorektálního karcinomu

Investor: Ministerstvo zdravotnictví ČR, Dlouhé nekódující RNA v exozómech jako biomarkery umožňující neinvazivní časnou detekci kolorektálního karcinomu, Podprogram 1 - standardní

90242, velká výzkumná infrastruktura

Název: CIISB III

Citovat

MACHÁČKOVÁ, Táňa, Petra VYCHYTILOVÁ, Marie BOUDNÁ, Karolína TRACHTOVÁ, Marketa PAVLIKOVA, Jan KOTOUCEK, Jana HALÁMKOVÁ, Dagmar AL TUKMACHI, Jiří ŠÁNA, Petra POKORNÁ, Milana ŠACHLOVÁ a Ondřej SLABÝ. Utility of RNA sequencing for transcriptome analysis of small extracellular vesicles derived from blood sera of colorectal cancer patients. In 114th Annual Meeting of the American Association for Cancer Research (AACR). 2023. Dostupné z: https://dx.doi.org/10.1158/1538-7445.AM2023-6709.

@proceedings{2393637,
   author = {Macháčková, Táňa and Vychytilová, Petra and Boudná, Marie and Trachtová, Karolína and Pavlikova, Marketa and Kotoucek, Jan and Halámková, Jana and Al Tukmachi, Dagmar and Šána, Jiří and Pokorná, Petra and Šachlová, Milana and Slabý, Ondřej},
   booktitle = {114th Annual Meeting of the American Association for Cancer Research (AACR)},
   doi = {http://dx.doi.org/10.1158/1538-7445.AM2023-6709},
   keywords = {colorectal cancer; biomarkers; non-coding RNAs; micro-RNA; small extracellular vesicles},
   language = {eng},
   title = {Utility of RNA sequencing for transcriptome analysis of small extracellular vesicles derived from blood sera of colorectal cancer patients},
   url = {https://aacrjournals.org/cancerres/article/83/7_Supplement/6709/723464/Abstract-6709-Utility-of-RNA-sequencing-for},
   year = {2023}
}

TY  - CONF
ID  - 2393637
AU  - Macháčková, Táňa - Vychytilová, Petra - Boudná, Marie - Trachtová, Karolína - Pavlikova, Marketa - Kotoucek, Jan - Halámková, Jana - Al Tukmachi, Dagmar - Šána, Jiří - Pokorná, Petra - Šachlová, Milana - Slabý, Ondřej
PY  - 2023
TI  - Utility of RNA sequencing for transcriptome analysis of small extracellular vesicles derived from blood sera of colorectal cancer patients
KW  - colorectal cancer
KW  - biomarkers
KW  - non-coding RNAs
KW  - micro-RNA
KW  - small extracellular vesicles
UR  - https://aacrjournals.org/cancerres/article/83/7_Supplement/6709/723464/Abstract-6709-Utility-of-RNA-sequencing-for
N2  - Currently used molecular diagnostic tests for colorectal cancer (CRC) are underperforming and more sensitive, non-invasive biomarkers are needed. Long non-coding RNAs (lncRNA) and microRNA (miRNA) have shown potential as diagnostic biomarkers. Unfortunately, the identification of non-coding RNA circulating biomarkers in blood serum is significantly burdened by abundant RNA specimens from disrupted blood cells. Recently, small extracellular vesicles (sEVs) emerged as potential reservoirs of clinically relevant biomarkers, including lncRNAs and miRNAs. In theory, sEVs protect RNAs from degradation and might serve as a source of intact RNA for further analyses. However, there is a lack of evidence supporting the superior quality of RNA extracted from sEVs over RNA from whole blood serum. This study aimed to analyze the RNA content of blood serum and the sEVs derived from the blood serum of CRC patients and healthy controls using RNA sequencing. Moreover, small RNA sequencing was used to evaluate the difference in miRNA profiles of sEVs and corresponding blood sera of CRC patients and healthy controls.
ER  -

MACHÁČKOVÁ, Táňa, Petra VYCHYTILOVÁ, Marie BOUDNÁ, Karolína TRACHTOVÁ, Marketa PAVLIKOVA, Jan KOTOUCEK, Jana HALÁMKOVÁ, Dagmar AL TUKMACHI, Jiří ŠÁNA, Petra POKORNÁ, Milana ŠACHLOVÁ a Ondřej SLABÝ. Utility of RNA sequencing for transcriptome analysis of small extracellular vesicles derived from blood sera of colorectal cancer patients. In \textit{114th Annual Meeting of the American Association for Cancer Research (AACR)}. 2023. Dostupné z: https://dx.doi.org/10.1158/1538-7445.AM2023-6709.

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