Changes in Sphingolipid Profile of Benzo[a]pyrene-Transformed
Human Bronchial Epithelial Cells Are Reflected in the Altered
Composition of Sphingolipids in Their Exosomes

J 2021

Changes in Sphingolipid Profile of Benzo[a]pyrene-Transformed Human Bronchial Epithelial Cells Are Reflected in the Altered Composition of Sphingolipids in Their Exosomes

MACHALA, Miroslav, Josef SLAVIK, Ondrej KOVAC, Jiřina PROCHÁZKOVÁ, Katerina PENCIKOVA et. al.

Základní údaje

Originální název

Changes in Sphingolipid Profile of Benzo[a]pyrene-Transformed Human Bronchial Epithelial Cells Are Reflected in the Altered Composition of Sphingolipids in Their Exosomes

Autoři

Vydání

International Journal of Molecular Sciences, Basel, Multidisciplinary Digital Publishing Institute, 2021, 1422-0067

Další údaje

Typ výsledku

Článek v odborném periodiku

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 6.208

DOI

http://dx.doi.org/10.3390/ijms22179195

UT WoS

000695576800001

Klíčová slova anglicky

sphingolipid; glycosphingolipid; eicosanoids; exosomes; in vitro cell transformation

Štítky

cancer, cellular plasticity, EMT, RIV ne, sphingolipids

Změněno: 24. 4. 2024 13:17, Mgr. Jiřina Procházková, Ph.D.

Anotace

V originále

Sphingolipids (SLs), glycosphingolipids (GSLs), and eicosanoids are bioactive lipids, which play important roles in the etiology of various diseases, including cancer. However, their content and roles in cancer cells, and in particular in the exosomes derived from tumor cells, remain insufficiently characterized. In this study, we evaluated alterations of SL and GSL levels in transformed cells and their exosomes, using comparative HPLC-MS/MS analysis of parental human bronchial epithelial cells HBEC-12KT and their derivative, benzo[a]pyrene-transformed HBEC-12KT-B1 cells with the acquired mesenchymal phenotype. We examined in parallel SL/GSL contents in the exosomes released from both cell lines. We found significant alterations of the SL/GSL profile in the transformed cell line, which corresponded well with alterations of the SL/GSL profile in exosomes derived from these cells. This suggested that a majority of SLs and GSLs were transported by exosomes in the same relative pattern as in the cells of origin. The only exceptions included decreased contents of sphingosin, sphingosin-1-phosphate, and lactosylceramide in exosomes derived from the transformed cells, as compared with the exosomes derived from the parental cell line. Importantly, we found increased levels of ceramide phosphate, globoside Gb3, and ganglioside GD3 in the exosomes derived from the transformed cells. These positive modulators of epithelial-mesenchymal transition and other pro-carcinogenic processes might thus also contribute to cancer progression in recipient cells. In addition, the transformed HBEC-12KT-B1 cells also produced increased amounts of eicosanoids, in particular prostaglandin E2. Taken together, the exosomes derived from the transformed cells with specifically upregulated SL and GSL species, and increased levels of eicosanoids, might contribute to changes within the cancer microenvironment and in recipient cells, which could in turn participate in cancer development. Future studies should address specific roles of individual SL and GSL species identified in the present study.

Citovat

MACHALA, Miroslav, Josef SLAVIK, Ondrej KOVAC, Jiřina PROCHÁZKOVÁ, Katerina PENCIKOVA, Martina PARENICOVA, Nicol STRAKOVA, Jan KOTOUCEK, Pavel KULICH, Steen MOLLERUP, Jan VONDRÁČEK a Martina HYZDALOVA. Changes in Sphingolipid Profile of Benzo[a]pyrene-Transformed Human Bronchial Epithelial Cells Are Reflected in the Altered Composition of Sphingolipids in Their Exosomes. International Journal of Molecular Sciences. Basel: Multidisciplinary Digital Publishing Institute, 2021, roč. 22, č. 17, 17 s. ISSN 1422-0067. Dostupné z: https://dx.doi.org/10.3390/ijms22179195.

@article{2396641,
   author = {Machala, Miroslav and Slavik, Josef and Kovac, Ondrej and Procházková, Jiřina and Pencikova, Katerina and Parenicova, Martina and Strakova, Nicol and Kotoucek, Jan and Kulich, Pavel and Mollerup, Steen and Vondráček, Jan and Hyzdalova, Martina},
   article_location = {Basel},
   article_number = {17},
   doi = {http://dx.doi.org/10.3390/ijms22179195},
   keywords = {sphingolipid; glycosphingolipid; eicosanoids; exosomes; in vitro cell transformation},
   issn = {1422-0067},
   journal = {International Journal of Molecular Sciences},
   title = {Changes in Sphingolipid Profile of Benzo[a]pyrene-Transformed Human Bronchial Epithelial Cells Are Reflected in the Altered Composition of Sphingolipids in Their Exosomes},
   volume = {22},
   year = {2021}
}

TY  - JOUR
ID  - 2396641
AU  - Machala, Miroslav - Slavik, Josef - Kovac, Ondrej - Procházková, Jiřina - Pencikova, Katerina - Parenicova, Martina - Strakova, Nicol - Kotoucek, Jan - Kulich, Pavel - Mollerup, Steen - Vondráček, Jan - Hyzdalova, Martina
PY  - 2021
TI  - Changes in Sphingolipid Profile of Benzo[a]pyrene-Transformed Human Bronchial Epithelial Cells Are Reflected in the Altered Composition of Sphingolipids in Their Exosomes
JF  - International Journal of Molecular Sciences
VL  - 22
IS  - 17
PB  - Multidisciplinary Digital Publishing Institute
SN  - 14220067
KW  - sphingolipid
KW  - glycosphingolipid
KW  - eicosanoids
KW  - exosomes
KW  - in vitro cell transformation
N2  - Sphingolipids (SLs), glycosphingolipids (GSLs), and eicosanoids are bioactive lipids, which play important roles in the etiology of various diseases, including cancer. However, their content and roles in cancer cells, and in particular in the exosomes derived from tumor cells, remain insufficiently characterized. In this study, we evaluated alterations of SL and GSL levels in transformed cells and their exosomes, using comparative HPLC-MS/MS analysis of parental human bronchial epithelial cells HBEC-12KT and their derivative, benzo[a]pyrene-transformed HBEC-12KT-B1 cells with the acquired mesenchymal phenotype. We examined in parallel SL/GSL contents in the exosomes released from both cell lines. We found significant alterations of the SL/GSL profile in the transformed cell line, which corresponded well with alterations of the SL/GSL profile in exosomes derived from these cells. This suggested that a majority of SLs and GSLs were transported by exosomes in the same relative pattern as in the cells of origin. The only exceptions included decreased contents of sphingosin, sphingosin-1-phosphate, and lactosylceramide in exosomes derived from the transformed cells, as compared with the exosomes derived from the parental cell line. Importantly, we found increased levels of ceramide phosphate, globoside Gb3, and ganglioside GD3 in the exosomes derived from the transformed cells. These positive modulators of epithelial-mesenchymal transition and other pro-carcinogenic processes might thus also contribute to cancer progression in recipient cells. In addition, the transformed HBEC-12KT-B1 cells also produced increased amounts of eicosanoids, in particular prostaglandin E2. Taken together, the exosomes derived from the transformed cells with specifically upregulated SL and GSL species, and increased levels of eicosanoids, might contribute to changes within the cancer microenvironment and in recipient cells, which could in turn participate in cancer development. Future studies should address specific roles of individual SL and GSL species identified in the present study.
ER  -

MACHALA, Miroslav, Josef SLAVIK, Ondrej KOVAC, Jiřina PROCHÁZKOVÁ, Katerina PENCIKOVA, Martina PARENICOVA, Nicol STRAKOVA, Jan KOTOUCEK, Pavel KULICH, Steen MOLLERUP, Jan VONDRÁČEK a Martina HYZDALOVA. Changes in Sphingolipid Profile of Benzo[a]pyrene-Transformed Human Bronchial Epithelial Cells Are Reflected in the Altered Composition of Sphingolipids in Their Exosomes. \textit{International Journal of Molecular Sciences}. Basel: Multidisciplinary Digital Publishing Institute, 2021, roč.~22, č.~17, 17 s. ISSN~1422-0067. Dostupné z: https://dx.doi.org/10.3390/ijms22179195.

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