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@article{2398879,
author = {Soukup, Jiri and Manethova, Monika and Stejskal, Vaclav and Novakova, Marie and Duskova, Jaroslava and Hornychova, Helena and Hacova, Maria and Staniczková Zambo, Iva and Zelinka, Tomas and Kosak, Mikulas and Cesak, Tomas and Netuka, David and Ryska, Ales and Gabalec, Filip},
article_location = {Totowa},
article_number = {1},
doi = {http://dx.doi.org/10.1007/s12022-024-09803-6},
keywords = {Hand2; dHand; Paraganglioma; Phox2B; GATA3; Neuroendocrine tumors},
language = {eng},
issn = {1046-3976},
journal = {Endocrine Pathology},
title = {Hand2 Immunohistochemistry in the Diagnosis of Paragangliomas and Other Neuroendocrine Neoplasms},
url = {https://link.springer.com/article/10.1007/s12022-024-09803-6},
volume = {35},
year = {2024}
}
TY - JOUR
ID - 2398879
AU - Soukup, Jiri - Manethova, Monika - Stejskal, Vaclav - Novakova, Marie - Duskova, Jaroslava - Hornychova, Helena - Hacova, Maria - Staniczková Zambo, Iva - Zelinka, Tomas - Kosak, Mikulas - Cesak, Tomas - Netuka, David - Ryska, Ales - Gabalec, Filip
PY - 2024
TI - Hand2 Immunohistochemistry in the Diagnosis of Paragangliomas and Other Neuroendocrine Neoplasms
JF - Endocrine Pathology
VL - 35
IS - 1
SP - 14-24
EP - 14-24
PB - Humana Press INC
SN - 10463976
KW - Hand2
KW - dHand
KW - Paraganglioma
KW - Phox2B
KW - GATA3
KW - Neuroendocrine tumors
UR - https://link.springer.com/article/10.1007/s12022-024-09803-6
N2 - Hand2 is a core transcription factor responsible for chromaffin cell differentiation. However, its potential utility in surgical pathology has not been studied. Thus, we aimed to investigate its expression in paragangliomas, other neuroendocrine neoplasms (NENs), and additional non-neuroendocrine tumors. We calibrated Hand2 immunohistochemistry on adrenal medulla cells and analyzed H-scores in 46 paragangliomas (PGs), 9 metastatic PGs, 21 cauda equina neuroendocrine tumors (CENETs), 48 neuroendocrine carcinomas (NECs), 8 olfactory neuroblastomas (ONBs), 110 well-differentiated NETs (WDNETs), 10 adrenal cortical carcinomas, 29 adrenal cortical adenomas, 8 melanomas, 41 different carcinomas, and 10 gastrointestinal stromal tumors (GISTs). Both tissue microarrays (TMAs) and whole sections (WSs) were studied. In 171 NENs, previously published data on Phox2B and GATA3 were correlated with Hand2. Hand2 was positive in 98.1% (54/55) PGs, but only rarely in WDNETs (9.6%, 10/104), CENETs (9.5%, 2/21), NECs (4.2%, 2/48), or ONBs (12.5%, 1/8). Any Hand2 positivity was 98.1% sensitive and 91.7% specific for the diagnosis of PG. The Hand2 H-score was significantly higher in primary PGs compared to Hand2-positive WDNETs (median 166.3 vs. 7.5; p < 0.0001). Metastatic PGs were positive in 88.9% (8/9). No Hand2 positivity was observed in any adrenal cortical neoplasm or other non-neuroendocrine tumors, with exception of 8/10 GISTs. Parasympathetic PGs showed a higher Hand2 H-score compared to sympathetic PGs (median H-scores 280 vs. 104, p < 0.0001). Hand2 positivity in NENs serves as a reliable marker of primary and metastatic PG, since other NENs only rarely exhibit limited Hand2 positivity.
ER -
SOUKUP, Jiri, Monika MANETHOVA, Vaclav STEJSKAL, Marie NOVAKOVA, Jaroslava DUSKOVA, Helena HORNYCHOVA, Maria HACOVA, Iva STANICZKOVÁ ZAMBO, Tomas ZELINKA, Mikulas KOSAK, Tomas CESAK, David NETUKA, Ales RYSKA and Filip GABALEC. Hand2 Immunohistochemistry in the Diagnosis of Paragangliomas and Other Neuroendocrine Neoplasms. \textit{Endocrine Pathology}. Totowa: Humana Press INC, 2024, vol.~35, No~1, p.~14-24. ISSN~1046-3976. Available from: https://dx.doi.org/10.1007/s12022-024-09803-6.
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