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@inbook{2400457, author = {Valuchová, Soňa and Mikulková, Pavlína and Pečinková, Jana and Říha, Karel}, address = {Humana New York, NY}, booktitle = {Plant Gametogenesis. Methods in Molecular Biology, vol 2484}, doi = {http://dx.doi.org/10.1007/978-1-0716-2253-7_8}, keywords = {Arabidopsis; fluorescences; microscopy; meiosis}, howpublished = {tištěná verze "print"}, language = {eng}, location = {Humana New York, NY}, isbn = {978-1-0716-2252-0}, pages = {93-105}, publisher = {Humana Press Inc.}, title = {Application of Chemical Inhibitors in Live Cell Imaging of Plant Meiosis Using Light Sheet Fluorescence Microscopy}, url = {https://link.springer.com/protocol/10.1007/978-1-0716-2253-7_8}, year = {2022} }
TY - CHAP ID - 2400457 AU - Valuchová, Soňa - Mikulková, Pavlína - Pečinková, Jana - Říha, Karel PY - 2022 TI - Application of Chemical Inhibitors in Live Cell Imaging of Plant Meiosis Using Light Sheet Fluorescence Microscopy VL - Plant Gametogenesis. Methods in Molecular Biology, vol 2484 PB - Humana Press Inc. CY - Humana New York, NY SN - 9781071622520 KW - Arabidopsis KW - fluorescences KW - microscopy KW - meiosis UR - https://link.springer.com/protocol/10.1007/978-1-0716-2253-7_8 N2 - Live imaging combined with the application of chemical inhibitors is a powerful research tool that enables researchers to precisely time the inhibition of cellular processes and study the consequences of these perturbations. This approach is usually applied to in vitro cultivated cells that are easily accessible to chemical treatments and microscopic observations. Here we describe a method for live cell imaging of Arabidopsis meiocytes embedded within floral organs combined with the application of a chemical drug at desired timepoints during meiosis. We describe a customized solution for the Zeiss Z.1 light sheet microscope, including sample preparation and data processing, and demonstrate its utility for the analysis of meiotic progression upon spindle inhibition. ER -
VALUCHOVÁ, Soňa, Pavlína MIKULKOVÁ, Jana PEČINKOVÁ a Karel ŘÍHA. Application of Chemical Inhibitors in Live Cell Imaging of Plant Meiosis Using Light Sheet Fluorescence Microscopy. In \textit{Plant Gametogenesis. Methods in Molecular Biology, vol 2484}. Humana New York, NY: Humana Press Inc., 2022, s.~93-105. Plant Gametogenesis. Methods in Molecular Biology, vol 2484. ISBN~978-1-0716-2252-0. Dostupné z: https://dx.doi.org/10.1007/978-1-0716-2253-7\_{}8.
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