Application of Chemical Inhibitors in Live Cell Imaging of
Plant Meiosis Using Light Sheet Fluorescence Microscopy

C 2022

Application of Chemical Inhibitors in Live Cell Imaging of Plant Meiosis Using Light Sheet Fluorescence Microscopy

VALUCHOVÁ, Soňa, Pavlína MIKULKOVÁ, Jana PEČINKOVÁ a Karel ŘÍHA

Základní údaje

Originální název

Application of Chemical Inhibitors in Live Cell Imaging of Plant Meiosis Using Light Sheet Fluorescence Microscopy

Autoři

VALUCHOVÁ, Soňa (703 Slovensko, domácí), Pavlína MIKULKOVÁ (203 Česká republika, domácí), Jana PEČINKOVÁ (203 Česká republika, domácí) a Karel ŘÍHA (203 Česká republika, garant, domácí)

Vydání

Humana New York, NY, Plant Gametogenesis. Methods in Molecular Biology, vol 2484, od s. 93-105, 13 s. Plant Gametogenesis. Methods in Molecular Biology, vol 2484, 2022

Nakladatel

Humana Press Inc.

Další údaje

Jazyk

angličtina

Typ výsledku

Kapitola resp. kapitoly v odborné knize

Obor

10611 Plant sciences, botany

Utajení

není předmětem státního či obchodního tajemství

Forma vydání

tištěná verze "print"

Odkazy

URL

Organizační jednotka

Středoevropský technologický institut

ISBN

978-1-0716-2252-0

DOI

http://dx.doi.org/10.1007/978-1-0716-2253-7_8

Klíčová slova anglicky

Arabidopsis; fluorescences; microscopy; meiosis

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 29. 5. 2024 11:03, Mgr. Eva Dubská

Anotace

V originále

Live imaging combined with the application of chemical inhibitors is a powerful research tool that enables researchers to precisely time the inhibition of cellular processes and study the consequences of these perturbations. This approach is usually applied to in vitro cultivated cells that are easily accessible to chemical treatments and microscopic observations. Here we describe a method for live cell imaging of Arabidopsis meiocytes embedded within floral organs combined with the application of a chemical drug at desired timepoints during meiosis. We describe a customized solution for the Zeiss Z.1 light sheet microscope, including sample preparation and data processing, and demonstrate its utility for the analysis of meiotic progression upon spindle inhibition.

Návaznosti

EF15_003/0000479, projekt VaV

Název: Regulace rostlinné meiózy

Citovat

VALUCHOVÁ, Soňa, Pavlína MIKULKOVÁ, Jana PEČINKOVÁ a Karel ŘÍHA. Application of Chemical Inhibitors in Live Cell Imaging of Plant Meiosis Using Light Sheet Fluorescence Microscopy. In Plant Gametogenesis. Methods in Molecular Biology, vol 2484. Humana New York, NY: Humana Press Inc., 2022, s. 93-105. Plant Gametogenesis. Methods in Molecular Biology, vol 2484. ISBN 978-1-0716-2252-0. Dostupné z: https://dx.doi.org/10.1007/978-1-0716-2253-7_8.

@inbook{2400457,
   author = {Valuchová, Soňa and Mikulková, Pavlína and Pečinková, Jana and Říha, Karel},
   address = {Humana New York, NY},
   booktitle = {Plant Gametogenesis. Methods in Molecular Biology, vol 2484},
   doi = {http://dx.doi.org/10.1007/978-1-0716-2253-7_8},
   keywords = {Arabidopsis; fluorescences; microscopy; meiosis},
   howpublished = {tištěná verze "print"},
   language = {eng},
   location = {Humana New York, NY},
   isbn = {978-1-0716-2252-0},
   pages = {93-105},
   publisher = {Humana Press Inc.},
   title = {Application of Chemical Inhibitors in Live Cell Imaging of Plant Meiosis Using Light Sheet Fluorescence Microscopy},
   url = {https://link.springer.com/protocol/10.1007/978-1-0716-2253-7_8},
   year = {2022}
}

TY  - CHAP
ID  - 2400457
AU  - Valuchová, Soňa - Mikulková, Pavlína - Pečinková, Jana - Říha, Karel
PY  - 2022
TI  - Application of Chemical Inhibitors in Live Cell Imaging of Plant Meiosis Using Light Sheet Fluorescence Microscopy
VL  - Plant Gametogenesis. Methods in Molecular Biology, vol 2484
PB  - Humana Press Inc.
CY  - Humana New York, NY
SN  - 9781071622520
KW  - Arabidopsis
KW  - fluorescences
KW  - microscopy
KW  - meiosis
UR  - https://link.springer.com/protocol/10.1007/978-1-0716-2253-7_8
N2  - Live imaging combined with the application of chemical inhibitors is a powerful research tool that enables researchers to precisely time the inhibition of cellular processes and study the consequences of these perturbations. This approach is usually applied to in vitro cultivated cells that are easily accessible to chemical treatments and microscopic observations. Here we describe a method for live cell imaging of Arabidopsis meiocytes embedded within floral organs combined with the application of a chemical drug at desired timepoints during meiosis. We describe a customized solution for the Zeiss Z.1 light sheet microscope, including sample preparation and data processing, and demonstrate its utility for the analysis of meiotic progression upon spindle inhibition.
ER  -

VALUCHOVÁ, Soňa, Pavlína MIKULKOVÁ, Jana PEČINKOVÁ a Karel ŘÍHA. Application of Chemical Inhibitors in Live Cell Imaging of Plant Meiosis Using Light Sheet Fluorescence Microscopy. In \textit{Plant Gametogenesis. Methods in Molecular Biology, vol 2484}. Humana New York, NY: Humana Press Inc., 2022, s.~93-105. Plant Gametogenesis. Methods in Molecular Biology, vol 2484. ISBN~978-1-0716-2252-0. Dostupné z: https://dx.doi.org/10.1007/978-1-0716-2253-7\_{}8.

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