Splicing analysis of STAT3 tandem donor suggests non-canonical
binding registers for U1 and U6 snRNAs

J 2024

Splicing analysis of STAT3 tandem donor suggests non-canonical binding registers for U1 and U6 snRNAs

KRAMÁREK, Michal, Přemysl SOUČEK, Kamila RÉBLOVÁ, Lucie KAJAN GRODECKA, Tomáš FREIBERGER et. al.

Základní údaje

Originální název

Splicing analysis of STAT3 tandem donor suggests non-canonical binding registers for U1 and U6 snRNAs

Autoři

KRAMÁREK, Michal (703 Slovensko, domácí), Přemysl SOUČEK (203 Česká republika, domácí), Kamila RÉBLOVÁ (203 Česká republika, domácí), Lucie KAJAN GRODECKA (203 Česká republika) a Tomáš FREIBERGER (203 Česká republika, domácí)

Vydání

Nucleic acids research, Oxford, Oxford University Press, 2024, 0305-1048

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30102 Immunology

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 14.900 v roce 2022

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1093/nar/gkae147

UT WoS

001176086700001

Klíčová slova anglicky

STAT3; snRNA

Štítky

14110114, 14110323, podil, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 2. 10. 2024 13:44, Mgr. Tereza Miškechová

Anotace

V originále

Tandem donor splice sites (5 ' ss) are unique regions with at least two GU dinucleotides serving as splicing cleavage sites. The Delta 3 tandem 5 ' ss are a specific subclass of 5 ' ss separated by 3 nucleotides which can affect protein function by inserting/deleting a single amino acid. One 5 ' ss is typically preferred, yet factors governing particular 5 ' ss choice are not fully understood. A highly conserved exon 21 of the STAT3 gene was chosen as a model to study Delta 3 tandem 5 ' ss splicing mechanisms. Based on multiple lines of experimental evidence, endogenous U1 snRNA most likely binds only to the upstream 5 ' ss. However, the downstream 5 ' ss is used preferentially, and the splice site choice is not dependent on the exact U1 snRNA binding position. Downstream 5 ' ss usage was sensitive to exact nucleotide composition and dependent on the presence of downstream regulatory region. The downstream 5 ' ss usage could be best explained by two novel interactions with endogenous U6 snRNA. U6 snRNA enables the downstream 5 ' ss usage in STAT3 exon 21 by two mechanisms: (i) binding in a novel non-canonical register and (ii) establishing extended Watson-Crick base pairing with the downstream regulatory region. This study suggests that U6:5 ' ss interaction is more flexible than previously thought.

Návaznosti

LM2018132, projekt VaV

Název: Národní centrum lékařské genomiky (Akronym: NCLG)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Národní centrum lékařské genomiky

MUNI/A/1244/2021, interní kód MU

Název: Vrozená imunita a její abnormality v rozvoji imunopatologických stavů

Investor: Masarykova univerzita, Vrozená imunita a její abnormality v rozvoji imunopatologických stavů

Citovat

KRAMÁREK, Michal, Přemysl SOUČEK, Kamila RÉBLOVÁ, Lucie KAJAN GRODECKA a Tomáš FREIBERGER. Splicing analysis of STAT3 tandem donor suggests non-canonical binding registers for U1 and U6 snRNAs. Nucleic acids research. Oxford: Oxford University Press, 2024, roč. 52, č. 10, s. 5959-5974. ISSN 0305-1048. Dostupné z: https://dx.doi.org/10.1093/nar/gkae147.

@article{2407804,
   author = {Kramárek, Michal and Souček, Přemysl and Réblová, Kamila and Kajan Grodecka, Lucie and Freiberger, Tomáš},
   article_location = {Oxford},
   article_number = {10},
   doi = {http://dx.doi.org/10.1093/nar/gkae147},
   keywords = {STAT3; snRNA},
   language = {eng},
   issn = {0305-1048},
   journal = {Nucleic acids research},
   title = {Splicing analysis of STAT3 tandem donor suggests non-canonical binding registers for U1 and U6 snRNAs},
   url = {https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkae147/7617147?login=true},
   volume = {52},
   year = {2024}
}

TY  - JOUR
ID  - 2407804
AU  - Kramárek, Michal - Souček, Přemysl - Réblová, Kamila - Kajan Grodecka, Lucie - Freiberger, Tomáš
PY  - 2024
TI  - Splicing analysis of STAT3 tandem donor suggests non-canonical binding registers for U1 and U6 snRNAs
JF  - Nucleic acids research
VL  - 52
IS  - 10
SP  - 5959-5974
EP  - 5959-5974
PB  - Oxford University Press
SN  - 03051048
KW  - STAT3
KW  - snRNA
UR  - https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkae147/7617147?login=true
N2  - Tandem donor splice sites (5 ' ss) are unique regions with at least two GU dinucleotides serving as splicing cleavage sites. The Delta 3 tandem 5 ' ss are a specific subclass of 5 ' ss separated by 3 nucleotides which can affect protein function by inserting/deleting a single amino acid. One 5 ' ss is typically preferred, yet factors governing particular 5 ' ss choice are not fully understood. A highly conserved exon 21 of the STAT3 gene was chosen as a model to study Delta 3 tandem 5 ' ss splicing mechanisms. Based on multiple lines of experimental evidence, endogenous U1 snRNA most likely binds only to the upstream 5 ' ss. However, the downstream 5 ' ss is used preferentially, and the splice site choice is not dependent on the exact U1 snRNA binding position. Downstream 5 ' ss usage was sensitive to exact nucleotide composition and dependent on the presence of downstream regulatory region. The downstream 5 ' ss usage could be best explained by two novel interactions with endogenous U6 snRNA. U6 snRNA enables the downstream 5 ' ss usage in STAT3 exon 21 by two mechanisms: (i) binding in a novel non-canonical register and (ii) establishing extended Watson-Crick base pairing with the downstream regulatory region. This study suggests that U6:5 ' ss interaction is more flexible than previously thought.
ER  -

KRAMÁREK, Michal, Přemysl SOUČEK, Kamila RÉBLOVÁ, Lucie KAJAN GRODECKA a Tomáš FREIBERGER. Splicing analysis of STAT3 tandem donor suggests non-canonical binding registers for U1 and U6 snRNAs. \textit{Nucleic acids research}. Oxford: Oxford University Press, 2024, roč.~52, č.~10, s.~5959-5974. ISSN~0305-1048. Dostupné z: https://dx.doi.org/10.1093/nar/gkae147.

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