Splicing analysis of STAT3 tandem donor suggests non-canonical
binding registers for U1 and U6 snRNAs

J 2024

Splicing analysis of STAT3 tandem donor suggests non-canonical binding registers for U1 and U6 snRNAs

KRAMÁREK, Michal; Přemysl SOUČEK; Kamila RÉBLOVÁ; Lucie KAJAN GRODECKA; Tomáš FREIBERGER et. al.

Základní údaje

Originální název

Splicing analysis of STAT3 tandem donor suggests non-canonical binding registers for U1 and U6 snRNAs

Autoři

KRAMÁREK, Michal (703 Slovensko, domácí); Přemysl SOUČEK (203 Česká republika, domácí); Kamila RÉBLOVÁ (203 Česká republika, domácí); Lucie KAJAN GRODECKA (203 Česká republika) a Tomáš FREIBERGER (203 Česká republika, domácí)

Vydání

Nucleic acids research, Oxford, Oxford University Press, 2024, 0305-1048

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30102 Immunology

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 16.700 v roce 2023

Kód RIV

RIV/00216224:14110/24:00136122

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1093/nar/gkae147

UT WoS

001176086700001

EID Scopus

2-s2.0-85195778811

Klíčová slova anglicky

STAT3; snRNA

Štítky

14110114, 14110323, CF GEN, podil, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 25. 3. 2025 18:21, Mgr. Eva Dubská

Anotace

V originále

Tandem donor splice sites (5 ' ss) are unique regions with at least two GU dinucleotides serving as splicing cleavage sites. The Delta 3 tandem 5 ' ss are a specific subclass of 5 ' ss separated by 3 nucleotides which can affect protein function by inserting/deleting a single amino acid. One 5 ' ss is typically preferred, yet factors governing particular 5 ' ss choice are not fully understood. A highly conserved exon 21 of the STAT3 gene was chosen as a model to study Delta 3 tandem 5 ' ss splicing mechanisms. Based on multiple lines of experimental evidence, endogenous U1 snRNA most likely binds only to the upstream 5 ' ss. However, the downstream 5 ' ss is used preferentially, and the splice site choice is not dependent on the exact U1 snRNA binding position. Downstream 5 ' ss usage was sensitive to exact nucleotide composition and dependent on the presence of downstream regulatory region. The downstream 5 ' ss usage could be best explained by two novel interactions with endogenous U6 snRNA. U6 snRNA enables the downstream 5 ' ss usage in STAT3 exon 21 by two mechanisms: (i) binding in a novel non-canonical register and (ii) establishing extended Watson-Crick base pairing with the downstream regulatory region. This study suggests that U6:5 ' ss interaction is more flexible than previously thought.

Návaznosti

MUNI/A/1244/2021, interní kód MU

Název: Vrozená imunita a její abnormality v rozvoji imunopatologických stavů

Investor: Masarykova univerzita, Vrozená imunita a její abnormality v rozvoji imunopatologických stavů

90132, velká výzkumná infrastruktura

Název: NCMG II

90254, velká výzkumná infrastruktura

Název: e-INFRA CZ II

Citovat

KRAMÁREK, Michal; Přemysl SOUČEK; Kamila RÉBLOVÁ; Lucie KAJAN GRODECKA a Tomáš FREIBERGER. Splicing analysis of STAT3 tandem donor suggests non-canonical binding registers for U1 and U6 snRNAs. Nucleic acids research. Oxford: Oxford University Press, 2024, roč. 52, č. 10, s. 5959-5974. ISSN 0305-1048. Dostupné z: https://dx.doi.org/10.1093/nar/gkae147.

@article{2407804,
   author = {Kramárek, Michal and Souček, Přemysl and Réblová, Kamila and Kajan Grodecka, Lucie and Freiberger, Tomáš},
   article_location = {Oxford},
   article_number = {10},
   doi = {http://dx.doi.org/10.1093/nar/gkae147},
   keywords = {STAT3; snRNA},
   language = {eng},
   issn = {0305-1048},
   journal = {Nucleic acids research},
   title = {Splicing analysis of STAT3 tandem donor suggests non-canonical binding registers for U1 and U6 snRNAs},
   url = {https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkae147/7617147?login=true},
   volume = {52},
   year = {2024}
}

TY  - JOUR
ID  - 2407804
AU  - Kramárek, Michal - Souček, Přemysl - Réblová, Kamila - Kajan Grodecka, Lucie - Freiberger, Tomáš
PY  - 2024
TI  - Splicing analysis of STAT3 tandem donor suggests non-canonical binding registers for U1 and U6 snRNAs
JF  - Nucleic acids research
VL  - 52
IS  - 10
SP  - 5959-5974
EP  - 5959-5974
PB  - Oxford University Press
SN  - 03051048
KW  - STAT3
KW  - snRNA
UR  - https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkae147/7617147?login=true
N2  - Tandem donor splice sites (5 ' ss) are unique regions with at least two GU dinucleotides serving as splicing cleavage sites. The Delta 3 tandem 5 ' ss are a specific subclass of 5 ' ss separated by 3 nucleotides which can affect protein function by inserting/deleting a single amino acid. One 5 ' ss is typically preferred, yet factors governing particular 5 ' ss choice are not fully understood. A highly conserved exon 21 of the STAT3 gene was chosen as a model to study Delta 3 tandem 5 ' ss splicing mechanisms. Based on multiple lines of experimental evidence, endogenous U1 snRNA most likely binds only to the upstream 5 ' ss. However, the downstream 5 ' ss is used preferentially, and the splice site choice is not dependent on the exact U1 snRNA binding position. Downstream 5 ' ss usage was sensitive to exact nucleotide composition and dependent on the presence of downstream regulatory region. The downstream 5 ' ss usage could be best explained by two novel interactions with endogenous U6 snRNA. U6 snRNA enables the downstream 5 ' ss usage in STAT3 exon 21 by two mechanisms: (i) binding in a novel non-canonical register and (ii) establishing extended Watson-Crick base pairing with the downstream regulatory region. This study suggests that U6:5 ' ss interaction is more flexible than previously thought.
ER  -

KRAMÁREK, Michal; Přemysl SOUČEK; Kamila RÉBLOVÁ; Lucie KAJAN GRODECKA a Tomáš FREIBERGER. Splicing analysis of STAT3 tandem donor suggests non-canonical binding registers for U1 and U6 snRNAs. \textit{Nucleic acids research}. Oxford: Oxford University Press, 2024, roč.~52, č.~10, s.~5959-5974. ISSN~0305-1048. Dostupné z: https://dx.doi.org/10.1093/nar/gkae147.

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