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@article{2407841,
author = {Carreira, Raquel and LamaandDiaz, Tomas and Crugeiras, Maria and Aguado, F Javier and Šebesta, Marek and Krejčí, Lumír and Blanco, Miguel G},
article_location = {OXFORD},
doi = {http://dx.doi.org/10.1093/nar/gkae453},
language = {eng},
issn = {0305-1048},
journal = {Nucleic Acids Research},
title = {Concurrent D-loop cleavage by Mus81 and Yen1 yields half-crossover precursors},
url = {https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkae453/7687436?login=true},
year = {2024}
}
TY - JOUR
ID - 2407841
AU - Carreira, Raquel - Lama-Diaz, Tomas - Crugeiras, Maria - Aguado, F Javier - Šebesta, Marek - Krejčí, Lumír - Blanco, Miguel G
PY - 2024
TI - Concurrent D-loop cleavage by Mus81 and Yen1 yields half-crossover precursors
JF - Nucleic Acids Research
PB - OXFORD UNIV PRESS
SN - 03051048
UR - https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkae453/7687436?login=true
N2 - Homologous recombination involves the formation of branched DNA molecules that may interfere with chromosome segregation. To resolve these persistent joint molecules, cells rely on the activation of structure-selective endonucleases (SSEs) during the late stages of the cell cycle. However, the premature activation of SSEs compromises genome integrity, due to untimely processing of replication and/or recombination intermediates. Here, we used a biochemical approach to show that the budding yeast SSEs Mus81 and Yen1 possess the ability to cleave the central recombination intermediate known as the displacement loop or D-loop. Moreover, we demonstrate that, consistently with previous genetic data, the simultaneous action of Mus81 and Yen1, followed by ligation, is sufficient to recreate the formation of a half-crossover precursor in vitro. Our results provide not only mechanistic explanation for the formation of a half-crossover, but also highlight the critical importance for precise regulation of these SSEs to prevent chromosomal rearrangements.
ER -
CARREIRA, Raquel, Tomas LAMA-DIAZ, Maria CRUGEIRAS, F Javier AGUADO, Marek ŠEBESTA, Lumír KREJČÍ a Miguel G BLANCO. Concurrent D-loop cleavage by Mus81 and Yen1 yields half-crossover precursors. \textit{Nucleic Acids Research}. OXFORD: OXFORD UNIV PRESS, 2024. ISSN~0305-1048. Dostupné z: https://dx.doi.org/10.1093/nar/gkae453.
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