Role of the 3-mercaptopyruvate sulfurtransferase in colon/
colorectal cancers

J 2024

Role of the 3-mercaptopyruvate sulfurtransferase in colon/ colorectal cancers

MATYASOVA, Katarina, Andrea SOLTYSOVA, Petr BABULA, Oľga KRIŽANOVÁ, Veronika LISKOVA et. al.

Základní údaje

Originální název

Role of the 3-mercaptopyruvate sulfurtransferase in colon/ colorectal cancers

Autoři

MATYASOVA, Katarina, Andrea SOLTYSOVA, Petr BABULA (203 Česká republika, domácí), Oľga KRIŽANOVÁ (703 Slovensko, domácí) a Veronika LISKOVA

Vydání

European Journal of Cell Biology, MUNICH, ELSEVIER GMBH, 2024, 0171-9335

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30105 Physiology

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 6.600 v roce 2022

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1016/j.ejcb.2024.151415

UT WoS

001230793100001

Klíčová slova anglicky

mercaptopyruvate sulfurtransferase; Fission; Colon/colorectal cancer; Mitochondria

Štítky

14110515, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 10. 6. 2024 10:11, Mgr. Tereza Miškechová

Anotace

V originále

The 3-mercaptopyruvate sulfurtransferase (MPST) is a protein persulfidase, occurring mainly in mitochondria. Although function of this protein in cancer cells has been already studied, no clear outcome can be postulated up to now. Therefore, we focused on the determination of function of MPST in colon (HCT116 cells)/colorectal (DLD1 cells) cancers. In silico analysis revealed that in gastrointestinal cancers, MPST together with its binding partners can be either of a high risk or might have a protective effect. Silencing of MPST gene resulted in decreased ATP, while acetyl-CoA levels were elevated. Increased apoptosis was detected in cells with silenced MPST gene, which was accompanied by decrease in mitochondrial membrane potential, but no changes in IP3 receptor's protein. Mitochondria underwent activation of fission and elevated DRP1 expression after MPST silencing. Proliferation and migration of DLD1 and HCT116 cells were markedly affected, showing the importance of MPST protein in colon/colorectal cancer development.

Citovat

MATYASOVA, Katarina, Andrea SOLTYSOVA, Petr BABULA, Oľga KRIŽANOVÁ a Veronika LISKOVA. Role of the 3-mercaptopyruvate sulfurtransferase in colon/ colorectal cancers. European Journal of Cell Biology. MUNICH: ELSEVIER GMBH, 2024, roč. 103, č. 2, s. 1-12. ISSN 0171-9335. Dostupné z: https://dx.doi.org/10.1016/j.ejcb.2024.151415.

@article{2409385,
   author = {Matyasova, Katarina and Soltysova, Andrea and Babula, Petr and Križanová, Oľga and Liskova, Veronika},
   article_location = {MUNICH},
   article_number = {2},
   doi = {http://dx.doi.org/10.1016/j.ejcb.2024.151415},
   keywords = {mercaptopyruvate sulfurtransferase; Fission; Colon/colorectal cancer; Mitochondria},
   language = {eng},
   issn = {0171-9335},
   journal = {European Journal of Cell Biology},
   title = {Role of the 3-mercaptopyruvate sulfurtransferase in colon/ colorectal cancers},
   url = {https://www.sciencedirect.com/science/article/pii/S0171933524000323?via%3Dihub},
   volume = {103},
   year = {2024}
}

TY  - JOUR
ID  - 2409385
AU  - Matyasova, Katarina - Soltysova, Andrea - Babula, Petr - Križanová, Oľga - Liskova, Veronika
PY  - 2024
TI  - Role of the 3-mercaptopyruvate sulfurtransferase in colon/ colorectal cancers
JF  - European Journal of Cell Biology
VL  - 103
IS  - 2
SP  - 1-12
EP  - 1-12
PB  - ELSEVIER GMBH
SN  - 01719335
KW  - mercaptopyruvate sulfurtransferase
KW  - Fission
KW  - Colon/colorectal cancer
KW  - Mitochondria
UR  - https://www.sciencedirect.com/science/article/pii/S0171933524000323?via%3Dihub
N2  - The 3-mercaptopyruvate sulfurtransferase (MPST) is a protein persulfidase, occurring mainly in mitochondria. Although function of this protein in cancer cells has been already studied, no clear outcome can be postulated up to now. Therefore, we focused on the determination of function of MPST in colon (HCT116 cells)/colorectal (DLD1 cells) cancers. In silico analysis revealed that in gastrointestinal cancers, MPST together with its binding partners can be either of a high risk or might have a protective effect. Silencing of MPST gene resulted in decreased ATP, while acetyl-CoA levels were elevated. Increased apoptosis was detected in cells with silenced MPST gene, which was accompanied by decrease in mitochondrial membrane potential, but no changes in IP3 receptor's protein. Mitochondria underwent activation of fission and elevated DRP1 expression after MPST silencing. Proliferation and migration of DLD1 and HCT116 cells were markedly affected, showing the importance of MPST protein in colon/colorectal cancer development.
ER  -

MATYASOVA, Katarina, Andrea SOLTYSOVA, Petr BABULA, Oľga KRIŽANOVÁ a Veronika LISKOVA. Role of the 3-mercaptopyruvate sulfurtransferase in colon/ colorectal cancers. \textit{European Journal of Cell Biology}. MUNICH: ELSEVIER GMBH, 2024, roč.~103, č.~2, s.~1-12. ISSN~0171-9335. Dostupné z: https://dx.doi.org/10.1016/j.ejcb.2024.151415.

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