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@article{2409437,
author = {Patakova, Petra and Branska, Barbora and Sedlar, Karel and Vasylkivska, Maryna and Jureckova, Katerina and Kolek, Jan and Koscova, Pavlina and Provaznik, Ivo},
article_location = {London},
article_number = {Feb},
doi = {http://dx.doi.org/10.1038/s41598-018-37679-0},
keywords = {ACETONE-BUTANOL FERMENTATION; CYCLOPROPANE FATTY-ACIDS; FACTOR HOMOLOGOUS GENES; BACILLUS-SUBTILIS; SOLVENT PRODUCTION; ESCHERICHIA-COLI; DIFFERENTIAL EXPRESSION; GROWTH TEMPERATURE; HISTIDINE KINASES; DIVISION SITE},
language = {eng},
issn = {2045-2322},
journal = {Nature Scientific Reports},
title = {Acidogenesis, solventogenesis, metabolic stress response and life cycle changes in <i>Clostridium beijerinckii</i> NRRL B-598 at the transcriptomic level},
url = {https://www.nature.com/articles/s41598-018-37679-0},
volume = {9},
year = {2019}
}
TY - JOUR
ID - 2409437
AU - Patakova, Petra - Branska, Barbora - Sedlar, Karel - Vasylkivska, Maryna - Jureckova, Katerina - Kolek, Jan - Koscova, Pavlina - Provaznik, Ivo
PY - 2019
TI - Acidogenesis, solventogenesis, metabolic stress response and life cycle changes in <i>Clostridium beijerinckii</i> NRRL B-598 at the transcriptomic level
JF - Nature Scientific Reports
VL - 9
IS - Feb
SP - 1-21
EP - 1-21
PB - NATURE RESEARCH
SN - 20452322
KW - ACETONE-BUTANOL FERMENTATION
KW - CYCLOPROPANE FATTY-ACIDS
KW - FACTOR HOMOLOGOUS GENES
KW - BACILLUS-SUBTILIS
KW - SOLVENT PRODUCTION
KW - ESCHERICHIA-COLI
KW - DIFFERENTIAL EXPRESSION
KW - GROWTH TEMPERATURE
KW - HISTIDINE KINASES
KW - DIVISION SITE
UR - https://www.nature.com/articles/s41598-018-37679-0
N2 - Clostridium beijerinckii NRRL B-598 is a sporulating, butanol and hydrogen producing strain that utilizes carbohydrates by the acetone-butanol-ethanol (ABE) fermentative pathway. The pathway consists of two metabolic phases, acidogenesis and solventogenesis, from which the latter one can be coupled with sporulation. Thorough transcriptomic profiling during a complete life cycle and both metabolic phases completed with flow cytometry, microscopy and a metabolites analysis helped to find out key genes involved in particular cellular events. The description of genes/operons that are closely involved in metabolism or the cell cycle is a necessary condition for metabolic engineering of the strain and will be valuable for all C. beijerinckii strains and other Clostridial species. The study focused on glucose transport and catabolism, hydrogen formation, metabolic stress response, binary fission, motility/chemotaxis and sporulation, which resulted in the composition of the unique image reflecting clostridial population changes. Surprisingly, the main change in expression of individual genes was coupled with the sporulation start and not with the transition from acidogenic to solventogenic metabolism. As expected, solvents formation started at pH decrease and the accumulation of butyric and acetic acids in the cultivation medium.
ER -
PATAKOVA, Petra, Barbora BRANSKA, Karel SEDLAR, Maryna VASYLKIVSKA, Katerina JURECKOVA, Jan KOLEK, Pavlina KOSCOVA a Ivo PROVAZNIK. Acidogenesis, solventogenesis, metabolic stress response and life cycle changes in \&{}lt;i\&{}gt;Clostridium beijerinckii\&{}lt;/i\&{}gt; NRRL B-598 at the transcriptomic level. \textit{Nature Scientific Reports}. London: NATURE RESEARCH, 2019, roč.~9, Feb, s.~1-21. ISSN~2045-2322. Dostupné z: https://dx.doi.org/10.1038/s41598-018-37679-0.
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