PATAKOVA, Petra, Maryna VASYLKIVSKA, Karel SEDLAR, Katerina JURECKOVA, Matěj BEZDÍČEK, Petra LOVECKA, Barbora BRANSKA, Petr KASTANEK a Karel KROFTA. Whole genome sequencing and characterization of <i>Pantoea agglomerans</i> DBM 3797, endophyte, isolated from fresh hop (<i>Humulus lupulus</i> L.). Frontiers in Microbiology. Switzerland: Frontiers Research Foundation, 2024, roč. 15, February 2024, s. 1-15. ISSN 1664-302X. Dostupné z: https://dx.doi.org/10.3389/fmicb.2024.1305338.
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Základní údaje
Originální název Whole genome sequencing and characterization of <i>Pantoea agglomerans</i> DBM 3797, endophyte, isolated from fresh hop (<i>Humulus lupulus</i> L.)
Autoři PATAKOVA, Petra (203 Česká republika), Maryna VASYLKIVSKA, Karel SEDLAR (203 Česká republika), Katerina JURECKOVA (203 Česká republika), Matěj BEZDÍČEK (203 Česká republika, domácí), Petra LOVECKA (203 Česká republika), Barbora BRANSKA (203 Česká republika), Petr KASTANEK (203 Česká republika) a Karel KROFTA (203 Česká republika).
Vydání Frontiers in Microbiology, Switzerland, Frontiers Research Foundation, 2024, 1664-302X.
Další údaje
Originální jazyk angličtina
Typ výsledku Článek v odborném periodiku
Obor 30205 Hematology
Stát vydavatele Švýcarsko
Utajení není předmětem státního či obchodního tajemství
WWW URL
Impakt faktor Impact factor: 5.200 v roce 2022
Organizační jednotka Lékařská fakulta
Doi http://dx.doi.org/10.3389/fmicb.2024.1305338
UT WoS 001173038100001
Klíčová slova anglicky Pantoea agglomerans; hops endophyte; plant growth promotion; genome characterization; gluconic acid
Štítky 14110212, rivok
Příznaky Mezinárodní význam, Recenzováno
Změnil Změnila: Mgr. Tereza Miškechová, učo 341652. Změněno: 10. 6. 2024 13:38.
Anotace
Background This paper brings new information about the genome and phenotypic characteristics of Pantoea agglomerans strain DBM 3797, isolated from fresh Czech hop (Humulus lupulus) in the Saaz hop-growing region. Although P. agglomerans strains are frequently isolated from different materials, there are not usually thoroughly characterized even if they have versatile metabolism and those isolated from plants may have a considerable potential for application in agriculture as a support culture for plant growth. Methods P. agglomerans DBM 3797 was cultured under aerobic and anaerobic conditions, its metabolites were analyzed by HPLC and it was tested for plant growth promotion abilities, such as phosphate solubilization, siderophore and indol-3-acetic acid productions. In addition, genomic DNA was extracted, sequenced and de novo assembly was performed. Further, genome annotation, pan-genome analysis and selected genome analyses, such as CRISPR arrays detection, antibiotic resistance and secondary metabolite genes identification were carried out. Results and discussion The typical appearance characteristics of the strain include the formation of symplasmata in submerged liquid culture and the formation of pale yellow colonies on agar. The genetic information of the strain (in total 4.8 Mb) is divided between a chromosome and two plasmids. The strain lacks any CRISPR-Cas system but is equipped with four restriction-modification systems. The phenotypic analysis focused on growth under both aerobic and anaerobic conditions, as well as traits associated with plant growth promotion. At both levels (genomic and phenotypic), the production of siderophores, indoleacetic acid-derived growth promoters, gluconic acid, and enzyme activities related to the degradation of complex organic compounds were found. Extracellular gluconic acid production under aerobic conditions (up to 8 g/l) is probably the result of glucose oxidation by the membrane-bound pyrroloquinoline quinone-dependent enzyme glucose dehydrogenase. The strain has a number of properties potentially beneficial to the hop plant and its closest relatives include the strains also isolated from the aerial parts of plants, yet its safety profile needs to be addressed in follow-up research.
VytisknoutZobrazeno: 21. 7. 2024 05:25