J 2019

Transcriptome analysis of human brain microvascular endothelial cells response to <i>Neisseria meningitidis</i> and its antigen MafA using RNA-seq

KANOVA, Evelina, Zuzana TKACOVA, Katarina BHIDE, Amod KULKARNI, Irene JIMENEZ-MUNGUIA et. al.

Základní údaje

Originální název

Transcriptome analysis of human brain microvascular endothelial cells response to <i>Neisseria meningitidis</i> and its antigen MafA using RNA-seq

Autoři

KANOVA, Evelina, Zuzana TKACOVA, Katarina BHIDE, Amod KULKARNI, Irene JIMENEZ-MUNGUIA, Patricia MERTINKOVA, Monika DRAZOVSKA, Punit TYAGI a Mangesh BHIDE

Vydání

Nature Scientific Reports, London, NATURE RESEARCH, 2019, 2045-2322

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Odkazy

Impakt faktor

Impact factor: 3.998

DOI

UT WoS

000502009000001

Klíčová slova anglicky

MEMBRANE VESICLE VACCINE; IV PILI; DEATH RECEPTORS; INFECTION; INVASION; ACTIVATION; CROSS; RECRUITMENT; GONORRHOEA; EXPRESSION

Štítky

Příznaky

Mezinárodní význam, Recenzováno
Změněno: 15. 10. 2024 09:08, Ing. Martina Blahová

Anotace

V originále

Interaction of Neisseria meningitidis (NM) with human brain microvascular endothelial cells (hBMECs) initiates of multiple cellular processes, which allow bacterial translocation across the blood-brain barrier (BBB). NM is equipped with several antigens, which interacts with the host cell receptors. Recently we have shown that adhesin MafA (UniProtKB-X5EG71), relatively less studied protein, is one of those surface exposed antigens that adhere to hBMECs. The present study was designed to comprehensively map the undergoing biological processes in hBMECs challenged with NM or MafA using RNA sequencing. 708 and 726 differentially expressed genes (DEGs) were identified in hBMECs exposed to NM and MafA, respectively. Gene ontology analysis of the DEGs revealed that several biological processes, which may alter the permeability of BBB, were activated. Comparative analysis of DEGs revealed that MafA, alike NM, might provoke TLR-dependent pathway and augment cytokine response. Moreover, both MafA and NM were able to induce genes involved in cell surface modifications, endocytosis, extracellular matrix remodulation and anoikis/apoptosis. In conclusion, this study for the first time describes effect of NM on the global gene expression in hBMECs using high-throughput RNA-seq. It also presents ability of MafA to induce gene expression, which might aid NM in breaching the BBB.