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@article{2409586, author = {Kanova, Evelina and Tkacova, Zuzana and Bhide, Katarina and Kulkarni, Amod and JimenezandMunguia, Irene and Mertinkova, Patricia and Drazovska, Monika and Tyagi, Punit and Bhide, Mangesh}, article_location = {London}, article_number = {Dec}, doi = {http://dx.doi.org/10.1038/s41598-019-55409-y}, keywords = {MEMBRANE VESICLE VACCINE; IV PILI; DEATH RECEPTORS; INFECTION; INVASION; ACTIVATION; CROSS; RECRUITMENT; GONORRHOEA; EXPRESSION}, language = {eng}, issn = {2045-2322}, journal = {Nature Scientific Reports}, title = {Transcriptome analysis of human brain microvascular endothelial cells response to <i>Neisseria meningitidis</i> and its antigen MafA using RNA-seq}, url = {https://www.nature.com/articles/s41598-019-55409-y}, volume = {9}, year = {2019} }
TY - JOUR ID - 2409586 AU - Kanova, Evelina - Tkacova, Zuzana - Bhide, Katarina - Kulkarni, Amod - Jimenez-Munguia, Irene - Mertinkova, Patricia - Drazovska, Monika - Tyagi, Punit - Bhide, Mangesh PY - 2019 TI - Transcriptome analysis of human brain microvascular endothelial cells response to <i>Neisseria meningitidis</i> and its antigen MafA using RNA-seq JF - Nature Scientific Reports VL - 9 IS - Dec SP - 1-16 EP - 1-16 PB - NATURE RESEARCH SN - 20452322 KW - MEMBRANE VESICLE VACCINE KW - IV PILI KW - DEATH RECEPTORS KW - INFECTION KW - INVASION KW - ACTIVATION KW - CROSS KW - RECRUITMENT KW - GONORRHOEA KW - EXPRESSION UR - https://www.nature.com/articles/s41598-019-55409-y N2 - Interaction of Neisseria meningitidis (NM) with human brain microvascular endothelial cells (hBMECs) initiates of multiple cellular processes, which allow bacterial translocation across the blood-brain barrier (BBB). NM is equipped with several antigens, which interacts with the host cell receptors. Recently we have shown that adhesin MafA (UniProtKB-X5EG71), relatively less studied protein, is one of those surface exposed antigens that adhere to hBMECs. The present study was designed to comprehensively map the undergoing biological processes in hBMECs challenged with NM or MafA using RNA sequencing. 708 and 726 differentially expressed genes (DEGs) were identified in hBMECs exposed to NM and MafA, respectively. Gene ontology analysis of the DEGs revealed that several biological processes, which may alter the permeability of BBB, were activated. Comparative analysis of DEGs revealed that MafA, alike NM, might provoke TLR-dependent pathway and augment cytokine response. Moreover, both MafA and NM were able to induce genes involved in cell surface modifications, endocytosis, extracellular matrix remodulation and anoikis/apoptosis. In conclusion, this study for the first time describes effect of NM on the global gene expression in hBMECs using high-throughput RNA-seq. It also presents ability of MafA to induce gene expression, which might aid NM in breaching the BBB. ER -
KANOVA, Evelina, Zuzana TKACOVA, Katarina BHIDE, Amod KULKARNI, Irene JIMENEZ-MUNGUIA, Patricia MERTINKOVA, Monika DRAZOVSKA, Punit TYAGI a Mangesh BHIDE. Transcriptome analysis of human brain microvascular endothelial cells response to \&{}lt;i\&{}gt;Neisseria meningitidis\&{}lt;/i\&{}gt; and its antigen MafA using RNA-seq. \textit{Nature Scientific Reports}. London: NATURE RESEARCH, 2019, roč.~9, Dec, s.~1-16. ISSN~2045-2322. Dostupné z: https://dx.doi.org/10.1038/s41598-019-55409-y.
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