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@article{2409597, author = {Domanska, Ausra and Flatt, Justin W and Jukonen, Joonas J J and Geraets, James A and Butcher, Sarah J}, article_location = {WASHINGTON}, article_number = {4}, doi = {http://dx.doi.org/10.1128/JVI.01597-18}, keywords = {cryo-EM; genome packaging; human parechovirus 3; neutralizing antibodies; picornavirus}, language = {eng}, issn = {0022-538X}, journal = {Journal of Virology}, title = {A 2.8-Angstrom-Resolution Cryo-Electron Microscopy Structure of Human Parechovirus 3 in Complex with Fab from a Neutralizing Antibody}, url = {https://www.pnas.org/doi/epdf/10.1073/pnas.1904732116}, volume = {93}, year = {2019} }
TY - JOUR ID - 2409597 AU - Domanska, Ausra - Flatt, Justin W - Jukonen, Joonas J J - Geraets, James A - Butcher, Sarah J PY - 2019 TI - A 2.8-Angstrom-Resolution Cryo-Electron Microscopy Structure of Human Parechovirus 3 in Complex with Fab from a Neutralizing Antibody JF - Journal of Virology VL - 93 IS - 4 PB - American Society for Microbiology SN - 0022538X KW - cryo-EM KW - genome packaging KW - human parechovirus 3 KW - neutralizing antibodies KW - picornavirus UR - https://www.pnas.org/doi/epdf/10.1073/pnas.1904732116 N2 - Human parechovirus 3 (HPeV3) infection is associated with sepsis characterized by significant immune activation and subsequent tissue damage in neonates. Strategies to limit infection have been unsuccessful due to inadequate molecular diagnostic tools for early detection and the lack of a vaccine or specific antiviral therapy. Toward the latter, we present a 2.8-angstrom-resolution structure of HPeV3 in complex with fragments from a neutralizing human monoclonal antibody, AT12-015, using cryo-electron microscopy (cryo-EM) and image reconstruction. Modeling revealed that the epitope extends across neighboring asymmetric units with contributions from capsid proteins VP0, VP1, and VP3. Antibody decoration was found to block binding of HPeV3 to cultured cells. Additionally, at high resolution, it was possible to model a stretch of RNA inside the virion and, from this, identify the key features that drive and stabilize protein-RNA association during assembly. ER -
DOMANSKA, Ausra, Justin W FLATT, Joonas J J JUKONEN, James A GERAETS a Sarah J BUTCHER. A 2.8-Angstrom-Resolution Cryo-Electron Microscopy Structure of Human Parechovirus 3 in Complex with Fab from a Neutralizing Antibody. \textit{Journal of Virology}. WASHINGTON: American Society for Microbiology, 2019, roč.~93, č.~4, 12 s. ISSN~0022-538X. Dostupné z: https://dx.doi.org/10.1128/JVI.01597-18.
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