J 2019

Transcriptomic Analysis Reveals the Roles of Detoxification Systems in Response to Mercury in <i>Chromera velia</i>

SHARAF, Abdoallah, De Michele ROBERTO, Ayush SHARMA, Safieh FAKHARI, Miroslav OBORNIK et. al.

Základní údaje

Originální název

Transcriptomic Analysis Reveals the Roles of Detoxification Systems in Response to Mercury in <i>Chromera velia</i>

Autoři

SHARAF, Abdoallah, De Michele ROBERTO, Ayush SHARMA, Safieh FAKHARI a Miroslav OBORNIK

Vydání

Biomolecules, Basel, Switzerland, MDPI AG, 2019, 2218-273X

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

Impakt faktor

Impact factor: 4.082

DOI

UT WoS

000502267900008

Klíčová slova anglicky

chromerids; transcriptome; heavy metal; antioxidant enzymes; xenobiotics; phylogenies; reactive oxygen species; reactive nitrogen species

Štítky

Příznaky

Mezinárodní význam, Recenzováno
Změněno: 10. 6. 2024 16:57, Mgr. Eva Dubská

Anotace

V originále

Heavy metal pollution is an increasing global concern. Among heavy metals, mercury (Hg) is especially dangerous because of its massive release into the environment and high toxicity, especially for aquatic organisms. The molecular response mechanisms of algae to Hg exposure are mostly unknown. Here, we combine physiological, biochemical, and transcriptomic analysis to provide, for the first time, a comprehensive view on the pathways activated in Chromera velia in response to toxic levels of Hg. Production of hydrogen peroxide and superoxide anion, two reactive oxygen species (ROS), showed opposite patterns in response to Hg2+ while reactive nitrogen species (RNS) levels did not change. A deep RNA sequencing analysis generated a total of 307,738,790 high-quality reads assembled in 122,874 transcripts, representing 89,853 unigenes successfully annotated in databases. Detailed analysis of the differently expressed genes corroborates the biochemical results observed in ROS production and suggests novel putative molecular mechanisms in the algal response to Hg2+. Moreover, we indicated that important transcription factor (TF) families associated with stress responses differentially expressed in C. velia cultures under Hg stress. Our study presents the first in-depth transcriptomic analysis of C. velia, focusing on the expression of genes involved in different detoxification defense systems in response to heavy metal stress.