DNA replication and chromosome positioning throughout the
interphase in three-dimensional space of plant nuclei

J 2020

DNA replication and chromosome positioning throughout the interphase in three-dimensional space of plant nuclei

NEMECKOVA, Alzbeta, Veronika KOLACKOVA, Jan VRANA, Jaroslav DOLEZEL, Eva HRIBOVA et. al.

Základní údaje

Originální název

DNA replication and chromosome positioning throughout the interphase in three-dimensional space of plant nuclei

Autoři

NEMECKOVA, Alzbeta, Veronika KOLACKOVA, Jan VRANA, Jaroslav DOLEZEL a Eva HRIBOVA

Vydání

Journal of Experimental Botany, Oxford, OXFORD UNIV PRESS, 2020, 0022-0957

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10611 Plant sciences, botany

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 6.992

DOI

http://dx.doi.org/10.1093/jxb/eraa370

UT WoS

000605983900008

Klíčová slova anglicky

DNA replication; EdU labeling; flow cytometry; Poaceae; Rabl configuration; S phase; three-dimensional fluorescence in situ hybridization (3D-FISH)

Štítky

CF CELLIM

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 9. 10. 2024 13:06, Mgr. Adéla Pešková

Anotace

V originále

Despite much recent progress, our understanding of the principles of plant genome organization and its dynamics in three-dimensional space of interphase nuclei remains surprisingly limited. Notably, it is not clear how these processes could be affected by the size of a plant's nuclear genome. In this study, DNA replication timing and interphase chromosome positioning were analyzed in seven Poaceae species that differ in their genome size. To provide a comprehensive picture, a suite of advanced, complementary methods was used: labeling of newly replicated DNA by ethynyl-2'-deoxyuridine, isolation of nuclei at particular cell cycle phases by flow cytometric sorting, three-dimensional immunofluorescence in situ hybridization, and confocal microscopy. Our results revealed conserved dynamics of DNA replication in all species, and a similar replication timing order for telomeres and centromeres, as well as for euchromatin and heterochromatin regions, irrespective of genome size. Moreover, stable chromosome positioning was observed while transitioning through different stages of interphase. These findings expand upon earlier studies in suggesting that a more complex interplay exists between genome size, organization of repetitive DNA sequences along chromosomes, and higher order chromatin structure and its maintenance in interphase, albeit controlled by currently unknown factors.

Citovat

NEMECKOVA, Alzbeta, Veronika KOLACKOVA, Jan VRANA, Jaroslav DOLEZEL a Eva HRIBOVA. DNA replication and chromosome positioning throughout the interphase in three-dimensional space of plant nuclei. Journal of Experimental Botany. Oxford: OXFORD UNIV PRESS, 2020, roč. 71, č. 20, s. 6262-6272. ISSN 0022-0957. Dostupné z: https://dx.doi.org/10.1093/jxb/eraa370.

@article{2409817,
   author = {Nemeckova, Alzbeta and Kolackova, Veronika and Vrana, Jan and Dolezel, Jaroslav and Hribova, Eva},
   article_location = {Oxford},
   article_number = {20},
   doi = {http://dx.doi.org/10.1093/jxb/eraa370},
   keywords = {DNA replication; EdU labeling; flow cytometry; Poaceae; Rabl configuration; S phase; three-dimensional fluorescence in situ hybridization (3D-FISH)},
   language = {eng},
   issn = {0022-0957},
   journal = {Journal of Experimental Botany},
   title = {DNA replication and chromosome positioning throughout the interphase in three-dimensional space of plant nuclei},
   url = {https://academic.oup.com/jxb/article/71/20/6262/5893557?login=true},
   volume = {71},
   year = {2020}
}

TY  - JOUR
ID  - 2409817
AU  - Nemeckova, Alzbeta - Kolackova, Veronika - Vrana, Jan - Dolezel, Jaroslav - Hribova, Eva
PY  - 2020
TI  - DNA replication and chromosome positioning throughout the interphase in three-dimensional space of plant nuclei
JF  - Journal of Experimental Botany
VL  - 71
IS  - 20
SP  - 6262-6272
EP  - 6262-6272
PB  - OXFORD UNIV PRESS
SN  - 00220957
KW  - DNA replication
KW  - EdU labeling
KW  - flow cytometry
KW  - Poaceae
KW  - Rabl configuration
KW  - S phase
KW  - three-dimensional fluorescence in situ hybridization (3D-FISH)
UR  - https://academic.oup.com/jxb/article/71/20/6262/5893557?login=true
N2  - Despite much recent progress, our understanding of the principles of plant genome organization and its dynamics in three-dimensional space of interphase nuclei remains surprisingly limited. Notably, it is not clear how these processes could be affected by the size of a plant's nuclear genome. In this study, DNA replication timing and interphase chromosome positioning were analyzed in seven Poaceae species that differ in their genome size. To provide a comprehensive picture, a suite of advanced, complementary methods was used: labeling of newly replicated DNA by ethynyl-2'-deoxyuridine, isolation of nuclei at particular cell cycle phases by flow cytometric sorting, three-dimensional immunofluorescence in situ hybridization, and confocal microscopy. Our results revealed conserved dynamics of DNA replication in all species, and a similar replication timing order for telomeres and centromeres, as well as for euchromatin and heterochromatin regions, irrespective of genome size. Moreover, stable chromosome positioning was observed while transitioning through different stages of interphase. These findings expand upon earlier studies in suggesting that a more complex interplay exists between genome size, organization of repetitive DNA sequences along chromosomes, and higher order chromatin structure and its maintenance in interphase, albeit controlled by currently unknown factors.
ER  -

NEMECKOVA, Alzbeta, Veronika KOLACKOVA, Jan VRANA, Jaroslav DOLEZEL a Eva HRIBOVA. DNA replication and chromosome positioning throughout the interphase in three-dimensional space of plant nuclei. \textit{Journal of Experimental Botany}. Oxford: OXFORD UNIV PRESS, 2020, roč.~71, č.~20, s.~6262-6272. ISSN~0022-0957. Dostupné z: https://dx.doi.org/10.1093/jxb/eraa370.

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