What is the impact of plastic deformation on cytocompatibility
of biodegradable Zn–Mg alloys?

J 2024

What is the impact of plastic deformation on cytocompatibility of biodegradable Zn–Mg alloys?

WOJTAS, Daniel, Klaudia TREMBECKA-WÓJCIGA, Magdalena GIELECIAK, Agnieszka BIGOS, Kamil BRUDECKI et. al.

Základní údaje

Originální název

What is the impact of plastic deformation on cytocompatibility of biodegradable Zn–Mg alloys?

Autoři

WOJTAS, Daniel (616 Polsko, domácí), Klaudia TREMBECKA-WÓJCIGA, Magdalena GIELECIAK, Agnieszka BIGOS, Kamil BRUDECKI, Sylwia PRZYBYSZ-GLOC, Romana SCHIRHAGL, Aldona MZYK a Anna JARZĘBSKA

Vydání

MATERIALS ADVANCES, CAMBRIDGE, ROYAL SOC CHEMISTRY, 2024, 2633-5409

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

20601 Medical engineering

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 5.000 v roce 2022

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1039/D4MA00098F

UT WoS

001250669600001

Klíčová slova anglicky

biodegradable Zn-Mg alloys; plastic deformation; cytocompatibility

Štítky

14110518, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 5. 11. 2024 14:08, Mgr. Tereza Miškechová

Anotace

V originále

Research on biodegradable zinc requires thorough in vitro cytotoxicity screening as the developed materials are being proposed for various medical implants, including stents. This study investigates the endothelial cell response to a novel Zn–0.8Mg alloy produced via hydrostatic extrusion (HSE), aiming to showcase the impact of plastic deformation on the cytocompatibility of biodegradable zinc-based materials. In doing so, the MTT test for cell viability studies as well as confocal laser scanning microscopy (CLSM) imaging for cell morphology analysis were used. In addition, a cutting-edge diamond-based quantum sensing technique, i.e., T1 relaxometry, was employed to reveal the nanoscale impact of ions on cells. It was demonstrated that the HSE-produced materials exhibited a 10-fold decrease in grain size, microstructural homogenization, and consequently more uniform degradation compared to hot extruded, coarse-grained materials. Despite these differences, the MTT and CLSM data did not show any drastic discrepancies between the endothelial cell response to any of the investigated materials. However, T1 relaxometry measurements indicated that plastic deformation might influence the cytocompatibility of biodegradable zinc-based materials, as evidenced by significant intracellular free radical production in endothelial cells exposed to ions released from the Zn–0.8Mg HSE alloy surface. Overall, no adverse effects of plastic deformation on the cytocompatibility of zinc-based materials were found as free radical generation may play a beneficial role in endothelial cell function, suggesting a complex interaction between material degradation and cellular response.

Citovat

WOJTAS, Daniel, Klaudia TREMBECKA-WÓJCIGA, Magdalena GIELECIAK, Agnieszka BIGOS, Kamil BRUDECKI, Sylwia PRZYBYSZ-GLOC, Romana SCHIRHAGL, Aldona MZYK a Anna JARZĘBSKA. What is the impact of plastic deformation on cytocompatibility of biodegradable Zn–Mg alloys? MATERIALS ADVANCES. CAMBRIDGE: ROYAL SOC CHEMISTRY, 2024, roč. 5, č. 14, s. 5958-5973. ISSN 2633-5409. Dostupné z: https://dx.doi.org/10.1039/D4MA00098F.

@article{2413698,
   author = {Wojtas, Daniel and TrembeckaandWójciga, Klaudia and Gieleciak, Magdalena and Bigos, Agnieszka and Brudecki, Kamil and PrzybyszandGloc, Sylwia and Schirhagl, Romana and Mzyk, Aldona and Jarzębska, Anna},
   article_location = {CAMBRIDGE},
   article_number = {14},
   doi = {http://dx.doi.org/10.1039/D4MA00098F},
   keywords = {biodegradable Zn-Mg alloys; plastic deformation; cytocompatibility},
   language = {eng},
   issn = {2633-5409},
   journal = {MATERIALS ADVANCES},
   title = {What is the impact of plastic deformation on cytocompatibility of biodegradable Zn–Mg alloys?},
   url = {http://pubs.rsc.org/en/content/articlelanding/2024/ma/d4ma00098f},
   volume = {5},
   year = {2024}
}

TY  - JOUR
ID  - 2413698
AU  - Wojtas, Daniel - Trembecka-Wójciga, Klaudia - Gieleciak, Magdalena - Bigos, Agnieszka - Brudecki, Kamil - Przybysz-Gloc, Sylwia - Schirhagl, Romana - Mzyk, Aldona - Jarzębska, Anna
PY  - 2024
TI  - What is the impact of plastic deformation on cytocompatibility of biodegradable Zn–Mg alloys?
JF  - MATERIALS ADVANCES
VL  - 5
IS  - 14
SP  - 5958-5973
EP  - 5958-5973
PB  - ROYAL SOC CHEMISTRY
SN  - 26335409
KW  - biodegradable Zn-Mg alloys
KW  - plastic deformation
KW  - cytocompatibility
UR  - http://pubs.rsc.org/en/content/articlelanding/2024/ma/d4ma00098f
N2  - Research on biodegradable zinc requires thorough in vitro cytotoxicity screening as the developed materials are being proposed for various medical implants, including stents. This study investigates the endothelial cell response to a novel Zn–0.8Mg alloy produced via hydrostatic extrusion (HSE), aiming to showcase the impact of plastic deformation on the cytocompatibility of biodegradable zinc-based materials. In doing so, the MTT test for cell viability studies as well as confocal laser scanning microscopy (CLSM) imaging for cell morphology analysis were used. In addition, a cutting-edge diamond-based quantum sensing technique, i.e., T1 relaxometry, was employed to reveal the nanoscale impact of ions on cells. It was demonstrated that the HSE-produced materials exhibited a 10-fold decrease in grain size, microstructural homogenization, and consequently more uniform degradation compared to hot extruded, coarse-grained materials. Despite these differences, the MTT and CLSM data did not show any drastic discrepancies between the endothelial cell response to any of the investigated materials. However, T1 relaxometry measurements indicated that plastic deformation might influence the cytocompatibility of biodegradable zinc-based materials, as evidenced by significant intracellular free radical production in endothelial cells exposed to ions released from the Zn–0.8Mg HSE alloy surface. Overall, no adverse effects of plastic deformation on the cytocompatibility of zinc-based materials were found as free radical generation may play a beneficial role in endothelial cell function, suggesting a complex interaction between material degradation and cellular response.
ER  -

WOJTAS, Daniel, Klaudia TREMBECKA-WÓJCIGA, Magdalena GIELECIAK, Agnieszka BIGOS, Kamil BRUDECKI, Sylwia PRZYBYSZ-GLOC, Romana SCHIRHAGL, Aldona MZYK a Anna JARZĘBSKA. What is the impact of plastic deformation on cytocompatibility of biodegradable Zn–Mg alloys? \textit{MATERIALS ADVANCES}. CAMBRIDGE: ROYAL SOC CHEMISTRY, 2024, roč.~5, č.~14, s.~5958-5973. ISSN~2633-5409. Dostupné z: https://dx.doi.org/10.1039/D4MA00098F.

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