An extensive immunohistochemical analysis of 290 ovarian adult
granulosa cell tumors with 29 markers

J 2024

An extensive immunohistochemical analysis of 290 ovarian adult granulosa cell tumors with 29 markers

NEMEJCOVA, Kristyna, Adam SAFANDA, Michaela KENDALL BARTU, Romana MICHALKOVA, Marian SVAJDLER et. al.

Základní údaje

Originální název

An extensive immunohistochemical analysis of 290 ovarian adult granulosa cell tumors with 29 markers

Autoři

Vydání

Virchows Archiv, New York, Springer, 2024, 0945-6317

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30109 Pathology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.500 v roce 2022

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1007/s00428-024-03854-0

UT WoS

001251527400001

Klíčová slova anglicky

Ovarian tumors; Sex cord-stromal tumors; Granulosa cell tumors; Immunohistochemistry

Štítky

14110230, 14110813

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 2. 7. 2024 08:20, Mgr. Tereza Miškechová

Anotace

V originále

The current knowledge about the immunohistochemical features of adult granulosa cell tumor (AGCT) is mostly limited to the "traditional" immunohistochemical markers of sex cord differentiation, such as inhibin, calretinin, FOXL2, SF1, and CD99. Knowledge about the immunohistochemical markers possibly used for predictive purpose is limited. In our study, we focused on the immunohistochemical examination of 290 cases of AGCT classified based on strict diagnostic criteria, including molecular testing. The antibodies used included 12 of the "diagnostic" antibodies already examined in previous studies, 10 antibodies whose expression has not yet been examined in AGCT, and 7 antibodies with possible predictive significance, including the expression of HER2, PD-L1, CTLA4, and 4 mismatch repair (MMR) proteins. The results of our study showed expression of FOXL2, SF1, CD99, inhibin A, calretinin, ER, PR, AR, CKAE1/3, and CAIX in 98%, 100%, 90%, 78%, 45%, 41%, 94%, 82%, 26%, and 9% of AGCT, respectively. GATA3, SATB2, napsin A, MUC4, TTF1, and CD44 were all negative. PTEN showed a loss of expression in 71% of cases and DPC4 in 4% of cases. The aberrant staining pattern (overexpression) of p53 was found in 1% (3/268) of cases, 2 primary tumors, and 1 recurrent case. Concerning the predictive markers, the results of our study showed that AGCT is microsatellite stable, do not express PD-L1, and are HER2 negative. The CTLA4 expression was found in almost 70% of AGCT tumor cells.

Návaznosti

LM2023033, projekt VaV

Název: Síť českých biobank

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, BBMRI.cz - Síť českých biobank

Citovat

NEMEJCOVA, Kristyna, Adam SAFANDA, Michaela KENDALL BARTU, Romana MICHALKOVA, Marian SVAJDLER, Tetiana SHATOKHINA, Jan LACO, Radoslav MATEJ, Gabor MEHES, Jana DROZENOVA, Jitka HAUSNEROVÁ, Zuzana SPURKOVA, Monika NÁLEŽINSKÁ a Pavel DUNDR. An extensive immunohistochemical analysis of 290 ovarian adult granulosa cell tumors with 29 markers. Virchows Archiv. New York: Springer, 2024, 11 s. ISSN 0945-6317. Dostupné z: https://dx.doi.org/10.1007/s00428-024-03854-0.

@article{2416237,
   author = {Nemejcova, Kristyna and Safanda, Adam and Kendall Bartu, Michaela and Michalkova, Romana and Svajdler, Marian and Shatokhina, Tetiana and Laco, Jan and Matej, Radoslav and Mehes, Gabor and Drozenova, Jana and Hausnerová, Jitka and Spurkova, Zuzana and Náležinská, Monika and Dundr, Pavel},
   article_location = {New York},
   doi = {http://dx.doi.org/10.1007/s00428-024-03854-0},
   keywords = {Ovarian tumors; Sex cord-stromal tumors; Granulosa cell tumors; Immunohistochemistry},
   language = {eng},
   issn = {0945-6317},
   journal = {Virchows Archiv},
   title = {An extensive immunohistochemical analysis of 290 ovarian adult granulosa cell tumors with 29 markers},
   url = {https://link.springer.com/article/10.1007/s00428-024-03854-0},
   year = {2024}
}

TY  - JOUR
ID  - 2416237
AU  - Nemejcova, Kristyna - Safanda, Adam - Kendall Bartu, Michaela - Michalkova, Romana - Svajdler, Marian - Shatokhina, Tetiana - Laco, Jan - Matej, Radoslav - Mehes, Gabor - Drozenova, Jana - Hausnerová, Jitka - Spurkova, Zuzana - Náležinská, Monika - Dundr, Pavel
PY  - 2024
TI  - An extensive immunohistochemical analysis of 290 ovarian adult granulosa cell tumors with 29 markers
JF  - Virchows Archiv
PB  - Springer
SN  - 09456317
KW  - Ovarian tumors
KW  - Sex cord-stromal tumors
KW  - Granulosa cell tumors
KW  - Immunohistochemistry
UR  - https://link.springer.com/article/10.1007/s00428-024-03854-0
N2  - The current knowledge about the immunohistochemical features of adult granulosa cell tumor (AGCT) is mostly limited to the "traditional" immunohistochemical markers of sex cord differentiation, such as inhibin, calretinin, FOXL2, SF1, and CD99. Knowledge about the immunohistochemical markers possibly used for predictive purpose is limited. In our study, we focused on the immunohistochemical examination of 290 cases of AGCT classified based on strict diagnostic criteria, including molecular testing. The antibodies used included 12 of the "diagnostic" antibodies already examined in previous studies, 10 antibodies whose expression has not yet been examined in AGCT, and 7 antibodies with possible predictive significance, including the expression of HER2, PD-L1, CTLA4, and 4 mismatch repair (MMR) proteins. The results of our study showed expression of FOXL2, SF1, CD99, inhibin A, calretinin, ER, PR, AR, CKAE1/3, and CAIX in 98%, 100%, 90%, 78%, 45%, 41%, 94%, 82%, 26%, and 9% of AGCT, respectively. GATA3, SATB2, napsin A, MUC4, TTF1, and CD44 were all negative. PTEN showed a loss of expression in 71% of cases and DPC4 in 4% of cases. The aberrant staining pattern (overexpression) of p53 was found in 1% (3/268) of cases, 2 primary tumors, and 1 recurrent case. Concerning the predictive markers, the results of our study showed that AGCT is microsatellite stable, do not express PD-L1, and are HER2 negative. The CTLA4 expression was found in almost 70% of AGCT tumor cells.
ER  -

NEMEJCOVA, Kristyna, Adam SAFANDA, Michaela KENDALL BARTU, Romana MICHALKOVA, Marian SVAJDLER, Tetiana SHATOKHINA, Jan LACO, Radoslav MATEJ, Gabor MEHES, Jana DROZENOVA, Jitka HAUSNEROVÁ, Zuzana SPURKOVA, Monika NÁLEŽINSKÁ a Pavel DUNDR. An extensive immunohistochemical analysis of 290 ovarian adult granulosa cell tumors with 29 markers. \textit{Virchows Archiv}. New York: Springer, 2024, 11 s. ISSN~0945-6317. Dostupné z: https://dx.doi.org/10.1007/s00428-024-03854-0.

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