Variants in the hERG channel associated with idiopathic
ventricular fibrillation: clinical, genetic, and functional
analysis

p 2024

Variants in the hERG channel associated with idiopathic ventricular fibrillation: clinical, genetic, and functional analysis

BÉBAROVÁ, Markéta, Natálie JANKOVÁ, Olga ŠVECOVÁ, Martin KRÁL, Jiří PACHERNÍK et. al.

Základní údaje

Originální název

Variants in the hERG channel associated with idiopathic ventricular fibrillation: clinical, genetic, and functional analysis

Autoři

BÉBAROVÁ, Markéta (203 Česká republika, garant, domácí), Natálie JANKOVÁ (203 Česká republika, domácí), Olga ŠVECOVÁ (203 Česká republika, domácí), Martin KRÁL (203 Česká republika, domácí), Jiří PACHERNÍK (203 Česká republika), Samuel LIETAVA (703 Slovensko, domácí), Jana ZÍDKOVÁ (203 Česká republika, domácí) a Tomáš NOVOTNÝ (203 Česká republika, domácí)

Vydání

15th New Frontiers in Basic Cardiovascular Research: A France-New EU Members Symposium, 2024

Další údaje

Jazyk

angličtina

Typ výsledku

Vyžádané přednášky

Obor

30201 Cardiac and Cardiovascular systems

Stát vydavatele

Francie

Utajení

není předmětem státního či obchodního tajemství

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

idiopathic ventricular fibrillation; hERG; genetic variant; patch clamp

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 20. 8. 2024 08:23, Mgr. Tereza Miškechová

Anotace

V originále

Objectives: In some patients suffering from idiopathic ventricular fibrillation (VF), variants in genes encoding cardiac ionic channels may be identified. A complex analysis has to be used to consider the possible pathogenic character of the identified variant. This study is focused on clinical, genetic, and functional analysis of a proband suffering from idiopathic VF and carrying two variants in the hERG (KCNH2) gene. Methods: After clinical and genetic investigation of the proband and family members, we have recently started electrophysiological analysis using whole-cell patch clamp technique at 37°C on Chinese hamster ovary cells transiently expressing human hERG channels, both wild type (WT) and carrying S1021Qfs*98 variant. Results: The proband, a female, was diagnosed at the age of 22 when she experienced her first attack of VF and was successfully resuscitated. QT interval corrected to the heart rate by the Bazett formula (QTc,B) and Fridericia formula (QTc,F) were 460 ms at rest. Qtc,B and QTc,F were 480 and 400 ms, respectively, during the exercise test and 460 and 420 ms, respectively, during the following recovery. No arrhythmia was detected during the exercise test as well as Holter monitoring. Echocardiography did not reveal any pathology as well. Hence, she was diagnosed as idiopathic VF, ICD was implanted, and she was treated using betaxolol. Repeated episodes of VF were reported during the following years (16 episodes per 7 years). Two hERG variants in trans, p. A228V and p.S1021Qfs*98, were identified in the proband, thus, functional analysis has been started. We have not identified any clear deviation in activation and deactivation of S1021Qfs*98 channels, but the tail current was significantly decreased by about 70 % in S1021Qfs*98 channels vs. WT channels. Currently, analysis of the channel inactivation proceeds. Conclusions: A proband suffering from idiopathic VF and carrying two hERG variants was identified and studied. Functional analysis of S1021Qfs*98 channels revealed a significantly decreased tail current with unaltered activation and deactivation gating. The functional analysis of A228V and co-expressed A228V/S1021Qfs*98 channels as well as analysis of channel expression and trafficking will follow.

Návaznosti

MUNI/A/1547/2023, interní kód MU

Název: Analýza (dys)funkce: od molekul k živému organismu

Investor: Masarykova univerzita, Analýza (dys)funkce: od molekul k živému organismu

NU22-02-00348, projekt VaV

Název: Funkční hodnocení genetických variant u případů klinicky „skutečné“ idiopatické fibrilace komor: in vitro a in silico modelování s cílem odhalit arytmogenní mechanismus

Investor: Ministerstvo zdravotnictví ČR, Funkční hodnocení genetických variant u případů klinicky „skutečné“ idiopatické fibrilace komor: in vitro a in silico modelování s cílem odhalit arytmogenní mechanismus, Podprogram 1 - standardní

Citovat

BÉBAROVÁ, Markéta, Natálie JANKOVÁ, Olga ŠVECOVÁ, Martin KRÁL, Jiří PACHERNÍK, Samuel LIETAVA, Jana ZÍDKOVÁ a Tomáš NOVOTNÝ. Variants in the hERG channel associated with idiopathic ventricular fibrillation: clinical, genetic, and functional analysis. In 15th New Frontiers in Basic Cardiovascular Research: A France-New EU Members Symposium. 2024.

@misc{2417401,
   author = {Bébarová, Markéta and Janková, Natálie and Švecová, Olga and Král, Martin and Pacherník, Jiří and Lietava, Samuel and Zídková, Jana and Novotný, Tomáš},
   booktitle = {15th New Frontiers in Basic Cardiovascular Research: A France-New EU Members Symposium},
   keywords = {idiopathic ventricular fibrillation; hERG; genetic variant; patch clamp},
   language = {eng},
   title = {Variants in the hERG channel associated with idiopathic ventricular fibrillation: clinical, genetic, and functional analysis},
   year = {2024}
}

TY  - SLIDE
ID  - 2417401
AU  - Bébarová, Markéta - Janková, Natálie - Švecová, Olga - Král, Martin - Pacherník, Jiří - Lietava, Samuel - Zídková, Jana - Novotný, Tomáš
PY  - 2024
TI  - Variants in the hERG channel associated with idiopathic ventricular fibrillation: clinical, genetic, and functional analysis
KW  - idiopathic ventricular fibrillation
KW  - hERG
KW  - genetic variant
KW  - patch clamp
N2  - Objectives: In some patients suffering from idiopathic ventricular fibrillation (VF), variants in genes encoding cardiac ionic channels may be identified. A complex analysis has to be used to consider the possible pathogenic character of the identified variant. This study is focused on clinical, genetic, and functional analysis of a proband suffering from idiopathic VF and carrying two variants in the hERG (KCNH2) gene. Methods: After clinical and genetic investigation of the proband and family members, we have recently started electrophysiological analysis using whole-cell patch clamp technique at 37°C on Chinese hamster ovary cells transiently expressing human hERG channels, both wild type (WT) and carrying S1021Qfs*98 variant. Results: The proband, a female, was diagnosed at the age of 22 when she experienced her first attack of VF and was successfully resuscitated. QT interval corrected to the heart rate by the Bazett formula (QTc,B) and Fridericia formula (QTc,F) were 460 ms at rest. Qtc,B and QTc,F were 480 and 400 ms, respectively, during the exercise test and 460 and 420 ms, respectively, during the following recovery. No arrhythmia was detected during the exercise test as well as Holter monitoring. Echocardiography did not reveal any pathology as well. Hence, she was diagnosed as idiopathic VF, ICD was implanted, and she was treated using betaxolol. Repeated episodes of VF were reported during the following years (16 episodes per 7 years). Two hERG variants in trans, p. A228V and p.S1021Qfs*98, were identified in the proband, thus, functional analysis has been started. We have not identified any clear deviation in activation and deactivation of S1021Qfs*98 channels, but the tail current was significantly decreased by about 70 % in S1021Qfs*98 channels vs. WT channels. Currently, analysis of the channel inactivation proceeds. Conclusions: A proband suffering from idiopathic VF and carrying two hERG variants was identified and studied. Functional analysis of S1021Qfs*98 channels revealed a significantly decreased tail current with unaltered activation and deactivation gating. The functional analysis of A228V and co-expressed A228V/S1021Qfs*98 channels as well as analysis of channel expression and trafficking will follow.
ER  -

BÉBAROVÁ, Markéta, Natálie JANKOVÁ, Olga ŠVECOVÁ, Martin KRÁL, Jiří PACHERNÍK, Samuel LIETAVA, Jana ZÍDKOVÁ a Tomáš NOVOTNÝ. Variants in the hERG channel associated with idiopathic ventricular fibrillation: clinical, genetic, and functional analysis. In \textit{15th New Frontiers in Basic Cardiovascular Research: A France-New EU Members Symposium}. 2024.

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