Proteomic Profiles of Whole Seeds, Hulls, and Dehulled Seeds of
Two Industrial Hemp (Cannabis sativa L.) Cultivars

J 2024

Proteomic Profiles of Whole Seeds, Hulls, and Dehulled Seeds of Two Industrial Hemp (Cannabis sativa L.) Cultivars

BÁRTA, Jan; Pavel ROUDNICKÝ; Markéta JAROŠOVÁ; Zbyněk ZDRÁHAL; Adéla STUPKOVÁ et. al.

Základní údaje

Originální název

Proteomic Profiles of Whole Seeds, Hulls, and Dehulled Seeds of Two Industrial Hemp (Cannabis sativa L.) Cultivars

Autoři

BÁRTA, Jan (203 Česká republika); Pavel ROUDNICKÝ (203 Česká republika, domácí); Markéta JAROŠOVÁ (203 Česká republika); Zbyněk ZDRÁHAL (203 Česká republika, garant, domácí); Adéla STUPKOVÁ; Veronika BÁRTOVÁ; Zlatuše KREJČOVÁ; Jan KYSELKA; Vladimír FILIP; Václav ŘÍHA; František LORENC; Jan BEDRNÍČEK a Pavel SMETANA

Vydání

PLANTS-BASEL, SWITZERLAND, MDPI, 2024, 2223-7747

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10611 Plant sciences, botany

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

fulltext

Impakt faktor

Impact factor: 4.000 v roce 2023

Kód RIV

RIV/00216224:14740/24:00136448

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.3390/plants13010111

UT WoS

001141311100001

EID Scopus

2-s2.0-85181937645

Klíčová slova anglicky

industrial hemp; hull; sdehulled seeds; proteomic profile; 11S globulin

Štítky

CF PROT, rivok

Změněno: 3. 6. 2025 12:16, Mgr. Eva Dubská

Anotace

V originále

As a source of nutritionally important components, hemp seeds are often dehulled for consumption and food applications by removing the hard hulls, which increases their nutritional value. The hulls thus become waste, although they may contain valuable protein items, about which there is a lack of information. The present work is therefore aimed at evaluating the proteome of hemp (Cannabis sativa L.) at the whole-seed, dehulled seed, and hull levels. The evaluation was performed on two cultivars, Santhica 27 and Uso-31, using LC-MS/MS analysis. In total, 2833 protein groups (PGs) were identified, and their relative abundances were determined. A set of 88 PGs whose abundance exceeded 1000 ppm (MP88 set) was considered for further evaluation. The PGs of the MP88 set were divided into ten protein classes. Seed storage proteins were found to be the most abundant protein class: the averages of the cultivars were 65.5%, 71.3%, and 57.5% for whole seeds, dehulled seeds, and hulls, respectively. In particular, 11S globulins representing edestin (three PGs) were found, followed by 7S vicilin-like proteins (four PGs) and 2S albumins (two PGs). The storage 11S globulins in Santhica 27 and Uso-31 were found to have a higher relative abundance in the dehulled seed proteome (summing to 58.6 and 63.2%) than in the hull proteome (50.5 and 54%), respectively. The second most abundant class of proteins was oleosins, which are part of oil-body membranes. PGs belonging to metabolic proteins (e.g., energy metabolism, nucleic acid metabolism, and protein synthesis) and proteins related to the defence and stress responses were more abundant in the hulls than in the dehulled seeds. The hulls can, therefore, be an essential source of proteins, especially for medical and biotechnological applications. Proteomic analysis has proven to be a valuable tool for studying differences in the relative abundance of proteins between dehulled hemp seeds and their hulls among different cultivars.

Návaznosti

EF16_026/0008446, projekt VaV

Název: Integrace signálu a epigenetické reprogramování pro produktivitu rostlin

90242, velká výzkumná infrastruktura

Název: CIISB III

90254, velká výzkumná infrastruktura

Název: e-INFRA CZ II

Citovat

BÁRTA, Jan; Pavel ROUDNICKÝ; Markéta JAROŠOVÁ; Zbyněk ZDRÁHAL; Adéla STUPKOVÁ; Veronika BÁRTOVÁ; Zlatuše KREJČOVÁ; Jan KYSELKA; Vladimír FILIP; Václav ŘÍHA; František LORENC; Jan BEDRNÍČEK a Pavel SMETANA. Proteomic Profiles of Whole Seeds, Hulls, and Dehulled Seeds of Two Industrial Hemp (Cannabis sativa L.) Cultivars. PLANTS-BASEL. SWITZERLAND: MDPI, 2024, roč. 13, č. 1, s. "111", 21 s. ISSN 2223-7747. Dostupné z: https://dx.doi.org/10.3390/plants13010111.

@article{2417797,
   author = {Bárta, Jan and Roudnický, Pavel and Jarošová, Markéta and Zdráhal, Zbyněk and Stupková, Adéla and Bártová, Veronika and Krejčová, Zlatuše and Kyselka, Jan and Filip, Vladimír and Říha, Václav and Lorenc, František and Bedrníček, Jan and Smetana, Pavel},
   article_location = {SWITZERLAND},
   article_number = {1},
   doi = {http://dx.doi.org/10.3390/plants13010111},
   keywords = {industrial hemp; hull; sdehulled seeds; proteomic profile; 11S globulin},
   language = {eng},
   issn = {2223-7747},
   journal = {PLANTS-BASEL},
   title = {Proteomic Profiles of Whole Seeds, Hulls, and Dehulled Seeds of Two Industrial Hemp (Cannabis sativa L.) Cultivars},
   url = {https://www.mdpi.com/2223-7747/13/1/111},
   volume = {13},
   year = {2024}
}

TY  - JOUR
ID  - 2417797
AU  - Bárta, Jan - Roudnický, Pavel - Jarošová, Markéta - Zdráhal, Zbyněk - Stupková, Adéla - Bártová, Veronika - Krejčová, Zlatuše - Kyselka, Jan - Filip, Vladimír - Říha, Václav - Lorenc, František - Bedrníček, Jan - Smetana, Pavel
PY  - 2024
TI  - Proteomic Profiles of Whole Seeds, Hulls, and Dehulled Seeds of Two Industrial Hemp (Cannabis sativa L.) Cultivars
JF  - PLANTS-BASEL
VL  - 13
IS  - 1
SP  - "111"
EP  - "111"
PB  - MDPI
SN  - 22237747
KW  - industrial hemp
KW  - hull
KW  - sdehulled seeds
KW  - proteomic profile
KW  - 11S globulin
UR  - https://www.mdpi.com/2223-7747/13/1/111
N2  - As a source of nutritionally important components, hemp seeds are often dehulled for consumption and food applications by removing the hard hulls, which increases their nutritional value. The hulls thus become waste, although they may contain valuable protein items, about which there is a lack of information. The present work is therefore aimed at evaluating the proteome of hemp (Cannabis sativa L.) at the whole-seed, dehulled seed, and hull levels. The evaluation was performed on two cultivars, Santhica 27 and Uso-31, using LC-MS/MS analysis. In total, 2833 protein groups (PGs) were identified, and their relative abundances were determined. A set of 88 PGs whose abundance exceeded 1000 ppm (MP88 set) was considered for further evaluation. The PGs of the MP88 set were divided into ten protein classes. Seed storage proteins were found to be the most abundant protein class: the averages of the cultivars were 65.5%, 71.3%, and 57.5% for whole seeds, dehulled seeds, and hulls, respectively. In particular, 11S globulins representing edestin (three PGs) were found, followed by 7S vicilin-like proteins (four PGs) and 2S albumins (two PGs). The storage 11S globulins in Santhica 27 and Uso-31 were found to have a higher relative abundance in the dehulled seed proteome (summing to 58.6 and 63.2%) than in the hull proteome (50.5 and 54%), respectively. The second most abundant class of proteins was oleosins, which are part of oil-body membranes. PGs belonging to metabolic proteins (e.g., energy metabolism, nucleic acid metabolism, and protein synthesis) and proteins related to the defence and stress responses were more abundant in the hulls than in the dehulled seeds. The hulls can, therefore, be an essential source of proteins, especially for medical and biotechnological applications. Proteomic analysis has proven to be a valuable tool for studying differences in the relative abundance of proteins between dehulled hemp seeds and their hulls among different cultivars.
ER  -

BÁRTA, Jan; Pavel ROUDNICKÝ; Markéta JAROŠOVÁ; Zbyněk ZDRÁHAL; Adéla STUPKOVÁ; Veronika BÁRTOVÁ; Zlatuše KREJČOVÁ; Jan KYSELKA; Vladimír FILIP; Václav ŘÍHA; František LORENC; Jan BEDRNÍČEK a Pavel SMETANA. Proteomic Profiles of Whole Seeds, Hulls, and Dehulled Seeds of Two Industrial Hemp (Cannabis sativa L.) Cultivars. \textit{PLANTS-BASEL}. SWITZERLAND: MDPI, 2024, roč.~13, č.~1, s.~''111'', 21 s. ISSN~2223-7747. Dostupné z: https://dx.doi.org/10.3390/plants13010111.

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