Proteomic Profile of Flaxseed (Linum usitatissimum L.) Products
as Influenced by Protein Concentration Method and Cultivar

J 2024

Proteomic Profile of Flaxseed (Linum usitatissimum L.) Products as Influenced by Protein Concentration Method and Cultivar

JAROŠOVÁ, Markéta, Pavel ROUDNICKÝ, Jan BÁRTA, Zbyněk ZDRÁHAL, Veronika BÁRTOVÁ et. al.

Základní údaje

Originální název

Proteomic Profile of Flaxseed (Linum usitatissimum L.) Products as Influenced by Protein Concentration Method and Cultivar

Autoři

JAROŠOVÁ, Markéta (203 Česká republika), Pavel ROUDNICKÝ (203 Česká republika, domácí), Jan BÁRTA (203 Česká republika), Zbyněk ZDRÁHAL (203 Česká republika, garant, domácí), Veronika BÁRTOVÁ (203 Česká republika), Adéla STUPKOVÁ (203 Česká republika), František LORENC (203 Česká republika), Marie BJELKOVÁ (203 Česká republika), Jan KYSELKA (203 Česká republika), Eva JAROŠOVÁ (203 Česká republika), Jan BEDRNÍČEK (203 Česká republika) a Andrea BOHATÁ (203 Česká republika)

Vydání

FOODS, SWITZERLAND, MDPI, 2024, 2304-8158

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10611 Plant sciences, botany

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

fulltext

Impakt faktor

Impact factor: 5.200 v roce 2022

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.3390/foods13091288

UT WoS

001220583900001

Klíčová slova anglicky

flaxseed; cultivar; flour; protein concentrate; sproteomic profile; 11S globulin

Změněno: 10. 7. 2024 11:13, Mgr. Ondrej Šedo, Ph.D.

Anotace

V originále

The research is focused on the quantitative evaluation of the flaxseed (Linum usitatissimum L.) proteome at the level of seed cake (SC), fine flour-sieved a fraction below 250 mu m (FF)-and protein concentrate (PC). The evaluation was performed on three oilseed flax cultivars (Agriol, Raciol, and Libra) with different levels of alpha-linolenic acid content using LC-MS/MS (shotgun proteomics) analysis, which was finalized by database searching using the NCBI protein database for Linum usitatissimum and related species. A total of 2560 protein groups (PGs) were identified, and their relative abundance was calculated. A set of 33 quantitatively most significant PGs was selected for further characterization. The selected PGs were divided into four classes-seed storage proteins (11S globulins and conlinins), oleosins, defense- and stress-related proteins, and other major proteins (mainly including enzymes). Seed storage proteins were found to be the most abundant proteins. Specifically, 11S globulins accounted for 41-44% of SC proteins, 40-46% of FF proteins, and 72-84% of PC proteins, depending on the cultivar. Conlinins (2S albumins) were the most abundant in FF, ranging from 10 to 13% (depending on cultivar). The second most important class from the point of relative abundance was oleosins, which were represented in SC and FF in the range of 2.1-3.8%, but only 0.36-1.20% in PC. Surprisingly, a relatively high abundance of chitinase was found in flax products as a protein related to defence and stress reactions.

Návaznosti

LM2023042, projekt VaV

Název: Česká infrastruktura pro integrativní strukturní biologii

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CIISB - Česká infrastruktura pro integrativní strukturní biologii

90254, velká výzkumná infrastruktura

Název: e-INFRA CZ II

Citovat

JAROŠOVÁ, Markéta, Pavel ROUDNICKÝ, Jan BÁRTA, Zbyněk ZDRÁHAL, Veronika BÁRTOVÁ, Adéla STUPKOVÁ, František LORENC, Marie BJELKOVÁ, Jan KYSELKA, Eva JAROŠOVÁ, Jan BEDRNÍČEK a Andrea BOHATÁ. Proteomic Profile of Flaxseed (Linum usitatissimum L.) Products as Influenced by Protein Concentration Method and Cultivar. FOODS. SWITZERLAND: MDPI, 2024, roč. 13, č. 9, s. "1288", 17 s. ISSN 2304-8158. Dostupné z: https://dx.doi.org/10.3390/foods13091288.

@article{2417825,
   author = {Jarošová, Markéta and Roudnický, Pavel and Bárta, Jan and Zdráhal, Zbyněk and Bártová, Veronika and Stupková, Adéla and Lorenc, František and Bjelková, Marie and Kyselka, Jan and Jarošová, Eva and Bedrníček, Jan and Bohatá, Andrea},
   article_location = {SWITZERLAND},
   article_number = {9},
   doi = {http://dx.doi.org/10.3390/foods13091288},
   keywords = {flaxseed; cultivar; flour; protein concentrate; sproteomic profile; 11S globulin},
   language = {eng},
   issn = {2304-8158},
   journal = {FOODS},
   title = {Proteomic Profile of Flaxseed (Linum usitatissimum L.) Products as Influenced by Protein Concentration Method and Cultivar},
   url = {https://www.mdpi.com/2304-8158/13/9/1288},
   volume = {13},
   year = {2024}
}

TY  - JOUR
ID  - 2417825
AU  - Jarošová, Markéta - Roudnický, Pavel - Bárta, Jan - Zdráhal, Zbyněk - Bártová, Veronika - Stupková, Adéla - Lorenc, František - Bjelková, Marie - Kyselka, Jan - Jarošová, Eva - Bedrníček, Jan - Bohatá, Andrea
PY  - 2024
TI  - Proteomic Profile of Flaxseed (Linum usitatissimum L.) Products as Influenced by Protein Concentration Method and Cultivar
JF  - FOODS
VL  - 13
IS  - 9
SP  - "1288"
EP  - "1288"
PB  - MDPI
SN  - 23048158
KW  - flaxseed
KW  - cultivar
KW  - flour
KW  - protein concentrate
KW  - sproteomic profile
KW  - 11S globulin
UR  - https://www.mdpi.com/2304-8158/13/9/1288
N2  - The research is focused on the quantitative evaluation of the flaxseed (Linum usitatissimum L.) proteome at the level of seed cake (SC), fine flour-sieved a fraction below 250 mu m (FF)-and protein concentrate (PC). The evaluation was performed on three oilseed flax cultivars (Agriol, Raciol, and Libra) with different levels of alpha-linolenic acid content using LC-MS/MS (shotgun proteomics) analysis, which was finalized by database searching using the NCBI protein database for Linum usitatissimum and related species. A total of 2560 protein groups (PGs) were identified, and their relative abundance was calculated. A set of 33 quantitatively most significant PGs was selected for further characterization. The selected PGs were divided into four classes-seed storage proteins (11S globulins and conlinins), oleosins, defense- and stress-related proteins, and other major proteins (mainly including enzymes). Seed storage proteins were found to be the most abundant proteins. Specifically, 11S globulins accounted for 41-44% of SC proteins, 40-46% of FF proteins, and 72-84% of PC proteins, depending on the cultivar. Conlinins (2S albumins) were the most abundant in FF, ranging from 10 to 13% (depending on cultivar). The second most important class from the point of relative abundance was oleosins, which were represented in SC and FF in the range of 2.1-3.8%, but only 0.36-1.20% in PC. Surprisingly, a relatively high abundance of chitinase was found in flax products as a protein related to defence and stress reactions.
ER  -

JAROŠOVÁ, Markéta, Pavel ROUDNICKÝ, Jan BÁRTA, Zbyněk ZDRÁHAL, Veronika BÁRTOVÁ, Adéla STUPKOVÁ, František LORENC, Marie BJELKOVÁ, Jan KYSELKA, Eva JAROŠOVÁ, Jan BEDRNÍČEK a Andrea BOHATÁ. Proteomic Profile of Flaxseed (Linum usitatissimum L.) Products as Influenced by Protein Concentration Method and Cultivar. \textit{FOODS}. SWITZERLAND: MDPI, 2024, roč.~13, č.~9, s.~''1288'', 17 s. ISSN~2304-8158. Dostupné z: https://dx.doi.org/10.3390/foods13091288.

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