Oral Neutrophil Free Fatty Acid Receptors Expression May Link
Oral Host and Microbiome Lipid Metabolism

J 2022

Oral Neutrophil Free Fatty Acid Receptors Expression May Link Oral Host and Microbiome Lipid Metabolism

WZATEK, Martin, Shaima BAHAMMAM, Petronela BUIGA, Kendal HADDAD, Corneliu SIMA et. al.

Základní údaje

Originální název

Oral Neutrophil Free Fatty Acid Receptors Expression May Link Oral Host and Microbiome Lipid Metabolism

Autoři

WZATEK, Martin (203 Česká republika, domácí), Shaima BAHAMMAM, Petronela BUIGA, Kendal HADDAD a Corneliu SIMA

Vydání

FRONTIERS IN ORAL HEALTH, LAUSANNE, FRONTIERS MEDIA SA, 2022, 2673-4842

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30208 Dentistry, oral surgery and medicine

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.3389/froh.2022.821326

UT WoS

000993703200001

Klíčová slova anglicky

oral neutrophils; free fatty acid receptor; FFAR; ERV1; fish intake

Štítky

14110130, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 11. 7. 2024 10:40, Mgr. Tereza Miškechová

Anotace

V originále

In health, commensal bacteria from oral biofilms stimulate polymorphonuclear neutrophil (PMN) recruitment in gingival sulci and the oral cavity. Oral PMN (oPMN) is short-lived cells with low prosurvival gene expression. In periodontitis, oPMN accumulates in higher numbers, has extended lifespan, and sustains nonresolving inflammation. We hypothesize that short- and long-chain free fatty acids (SCFAs and LCFAs) and lipid mediator resolvin E1 (RvE1) modulate host ability to control biofilms and resolve inflammation. Our objective was to measure oPMN surface expression of receptors FFAR2 (binds bacteria-derived SCFA), FFAR4 (binds LCFA, EPA, and DHA), and ERV1 (binds RvE1) in health and to assess sex differences. We included 20 periodontally healthy individuals aged 20-80 years (10 males, 10 females), who were asked to (1) answer a targeted health nutritional questionnaire and (2) provide an oral saline rinse. oPMN isolated by sequential filtration was labeled with fluorophore-conjugated antibodies against CD11b, CD14, CD16, CD66b, ERV1, FFAR2, and FFAR4 and analyzed by flow cytometry. Statistical analyses were the following: two-way ANOVA, Tukey's test, and Pearson's correlation. Oral rinses contained 80% oPMN of which 60% were ERV1(+) and FFAR2(+), and 10% FFAR4(+), with no sex differences. Females had more oPMN ERV1 compared to males. Both sexes had higher ERV1 compared to FFAR2 and FFAR4. CD66b(+)CD16(high) oPMN expressed less ERV1 and FFAR2 compared to CD66b(+)CD16(low). There were positive correlations between oPMN ERV1 and FFAR2 expression and between ERV1(+) and FFAR2(+) oPMN and fish intake. These findings will help to better understand how oral host and microbiome interactions maintain periodontal health.

Citovat

WZATEK, Martin, Shaima BAHAMMAM, Petronela BUIGA, Kendal HADDAD a Corneliu SIMA. Oral Neutrophil Free Fatty Acid Receptors Expression May Link Oral Host and Microbiome Lipid Metabolism. FRONTIERS IN ORAL HEALTH. LAUSANNE: FRONTIERS MEDIA SA, 2022, roč. 3, March 2022, s. 1-11. ISSN 2673-4842. Dostupné z: https://dx.doi.org/10.3389/froh.2022.821326.

@article{2417968,
   author = {Wzatek, Martin and Bahammam, Shaima and Buiga, Petronela and Haddad, Kendal and Sima, Corneliu},
   article_location = {LAUSANNE},
   article_number = {March 2022},
   doi = {http://dx.doi.org/10.3389/froh.2022.821326},
   keywords = {oral neutrophils; free fatty acid receptor; FFAR; ERV1; fish intake},
   language = {eng},
   issn = {2673-4842},
   journal = {FRONTIERS IN ORAL HEALTH},
   title = {Oral Neutrophil Free Fatty Acid Receptors Expression May Link Oral Host and Microbiome Lipid Metabolism},
   url = {https://www.frontiersin.org/journals/oral-health/articles/10.3389/froh.2022.821326/full},
   volume = {3},
   year = {2022}
}

TY  - JOUR
ID  - 2417968
AU  - Wzatek, Martin - Bahammam, Shaima - Buiga, Petronela - Haddad, Kendal - Sima, Corneliu
PY  - 2022
TI  - Oral Neutrophil Free Fatty Acid Receptors Expression May Link Oral Host and Microbiome Lipid Metabolism
JF  - FRONTIERS IN ORAL HEALTH
VL  - 3
IS  - March 2022
SP  - 1-11
EP  - 1-11
PB  - FRONTIERS MEDIA SA
SN  - 26734842
KW  - oral neutrophils
KW  - free fatty acid receptor
KW  - FFAR
KW  - ERV1
KW  - fish intake
UR  - https://www.frontiersin.org/journals/oral-health/articles/10.3389/froh.2022.821326/full
N2  - In health, commensal bacteria from oral biofilms stimulate polymorphonuclear neutrophil (PMN) recruitment in gingival sulci and the oral cavity. Oral PMN (oPMN) is short-lived cells with low prosurvival gene expression. In periodontitis, oPMN accumulates in higher numbers, has extended lifespan, and sustains nonresolving inflammation. We hypothesize that short- and long-chain free fatty acids (SCFAs and LCFAs) and lipid mediator resolvin E1 (RvE1) modulate host ability to control biofilms and resolve inflammation. Our objective was to measure oPMN surface expression of receptors FFAR2 (binds bacteria-derived SCFA), FFAR4 (binds LCFA, EPA, and DHA), and ERV1 (binds RvE1) in health and to assess sex differences. We included 20 periodontally healthy individuals aged 20-80 years (10 males, 10 females), who were asked to (1) answer a targeted health nutritional questionnaire and (2) provide an oral saline rinse. oPMN isolated by sequential filtration was labeled with fluorophore-conjugated antibodies against CD11b, CD14, CD16, CD66b, ERV1, FFAR2, and FFAR4 and analyzed by flow cytometry. Statistical analyses were the following: two-way ANOVA, Tukey's test, and Pearson's correlation. Oral rinses contained 80% oPMN of which 60% were ERV1(+) and FFAR2(+), and 10% FFAR4(+), with no sex differences. Females had more oPMN ERV1 compared to males. Both sexes had higher ERV1 compared to FFAR2 and FFAR4. CD66b(+)CD16(high) oPMN expressed less ERV1 and FFAR2 compared to CD66b(+)CD16(low). There were positive correlations between oPMN ERV1 and FFAR2 expression and between ERV1(+) and FFAR2(+) oPMN and fish intake. These findings will help to better understand how oral host and microbiome interactions maintain periodontal health.
ER  -

WZATEK, Martin, Shaima BAHAMMAM, Petronela BUIGA, Kendal HADDAD a Corneliu SIMA. Oral Neutrophil Free Fatty Acid Receptors Expression May Link Oral Host and Microbiome Lipid Metabolism. \textit{FRONTIERS IN ORAL HEALTH}. LAUSANNE: FRONTIERS MEDIA SA, 2022, roč.~3, March 2022, s.~1-11. ISSN~2673-4842. Dostupné z: https://dx.doi.org/10.3389/froh.2022.821326.

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