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@article{2421457,
author = {Odehnalova, Klara and Pribilova, Petra and Maršálek, Blahoslav and Babica, Pavel},
article_location = {UNITED STATES},
article_number = {2},
doi = {http://dx.doi.org/10.1093/jat/bkad092},
keywords = {PARALYTIC SHELL; FISH TOXINS; SOLID-PHASE EXTRACTION; HUMAN URINE; NEOSAXITOXIN; ACCUMULATION; CYANOBACTERIAL; IDENTIFICATION; SERUM},
language = {eng},
issn = {0146-4760},
journal = {JOURNAL OF ANALYTICAL TOXICOLOGY},
title = {A fast and reliable LC-MS-MS method for the quantification of saxitoxin in blood plasma samples},
url = {https://academic.oup.com/jat/article/48/2/119/7510958?login=true},
volume = {48},
year = {2024}
}
TY - JOUR
ID - 2421457
AU - Odehnalova, Klara - Pribilova, Petra - Maršálek, Blahoslav - Babica, Pavel
PY - 2024
TI - A fast and reliable LC-MS-MS method for the quantification of saxitoxin in blood plasma samples
JF - JOURNAL OF ANALYTICAL TOXICOLOGY
VL - 48
IS - 2
SP - 119-125
EP - 119-125
PB - OXFORD UNIV PRESS INC
SN - 01464760
KW - PARALYTIC SHELL
KW - FISH TOXINS
KW - SOLID-PHASE EXTRACTION
KW - HUMAN URINE
KW - NEOSAXITOXIN
KW - ACCUMULATION
KW - CYANOBACTERIAL
KW - IDENTIFICATION
KW - SERUM
UR - https://academic.oup.com/jat/article/48/2/119/7510958?login=true
N2 - Saxitoxins (STXs) are potent neurotoxins produced by marine dinoflagellates or freshwater cyanobacteria, known to cause acute and eventually fatal human intoxications, which are classified as paralytic shellfish poisonings (PSPs). Rapid analysis of STXs in blood plasma can be used for a timely diagnosis and confirmation of PSPs. We developed a fast and simple method of STX extraction based on plasma sample acidification and precipitation by acetonitrile, followed by quantification using liquid chromatography-tandem mass spectrometry (LC-MS-MS). Our approach provides the results <= 30 min, with a limit of detection of 2.8 ng/mL and a lower limit of quantification of 5.0 ng/mL. Within-run and between-run precision experiments showed good reproducibility with <= 15% values. Standard curves for calibration were linear with correlation coefficients >= 0.98 across the assay calibration range (5-200 ng/mL). In an interlaboratory analytical exercise, the method was found to be 100% accurate in determining the presence or absence of STX in human plasma specimens, with recovery values of 86-99%. This simple method for STX determination in animal or human plasma can quickly and reliably diagnose STX exposures and confirm suspected PSP cases to facilitate patient treatment or expedite necessary public health or security actions.
ER -
ODEHNALOVA, Klara, Petra PRIBILOVA, Blahoslav MARŠÁLEK a Pavel BABICA. A fast and reliable LC-MS-MS method for the quantification of saxitoxin in blood plasma samples. \textit{JOURNAL OF ANALYTICAL TOXICOLOGY}. UNITED STATES: OXFORD UNIV PRESS INC, 2024, roč.~48, č.~2, s.~119-125. ISSN~0146-4760. Dostupné z: https://dx.doi.org/10.1093/jat/bkad092.
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