Zygotic spindle orientation defines cleavage pattern and
nuclear status of human embryos

J 2024

Zygotic spindle orientation defines cleavage pattern and nuclear status of human embryos

POROKH, Volodymyr, Drahomira KYJOVSKA, Martina MARTONOVA, Tereza KLENKOVA, Pavel OTEVREL et. al.

Základní údaje

Originální název

Zygotic spindle orientation defines cleavage pattern and nuclear status of human embryos

Autoři

POROKH, Volodymyr (804 Ukrajina, domácí), Drahomira KYJOVSKA (203 Česká republika), Martina MARTONOVA (203 Česká republika), Tereza KLENKOVA (203 Česká republika), Pavel OTEVREL (203 Česká republika), Soňa KLOUDOVÁ (203 Česká republika, domácí) a Zuzana HOLUBCOVÁ (203 Česká republika, domácí)

Vydání

Nature Communications, Berlin, Nature, 2024, 2041-1723

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10605 Developmental biology

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 16.600 v roce 2022

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1038/s41467-024-50732-z

UT WoS

001280592000005

Klíčová slova anglicky

zygotic spindle orientation; human embryos; cleavage pattern; nuclear status

Štítky

14110517, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 15. 8. 2024 09:06, Mgr. Tereza Miškechová

Anotace

V originále

The first embryonic division represents a starting point for the development of a new individual. In many species, tight control over the first embryonic division ensures its accuracy. However, the first division in humans is often erroneous and can impair embryo development. To delineate the spatiotemporal organization of the first mitotic division typical for normal human embryo development, we systematically analyzed a unique timelapse dataset of 300 IVF embryos that developed into healthy newborns. The zygotic division pattern of these best-quality embryos was compared to their siblings that failed to implant or arrested during cleavage stage. We show that division at the right angle to the juxtaposed pronuclei is preferential and supports faithful zygotic division. Alternative configurations of the first mitosis are associated with reduced clustering of nucleoli and multinucleation at the 2-cell stage, which are more common in women of advanced age. Collectively, these data imply that orientation of the first division predisposes human embryos to genetic (in)stability and may contribute to aneuploidy and age-related infertility.

Návaznosti

MUNI/A/1301/2022, interní kód MU

Název: Zdroje pro tkáňové inženýrství 13

Investor: Masarykova univerzita, Zdroje pro tkáňové inženýrství 13

MUNI/A/1598/2023, interní kód MU

Název: Zdroje pro tkáňové inženýrství 14

Investor: Masarykova univerzita, Zdroje pro tkáňové inženýrství 14

MUNI/IGA/1127/2021, interní kód MU

Název: Molecular organization of the zygotic spindle apparatus in non-rodent embryos

Investor: Masarykova univerzita, Molecular organization of the zygotic spindle apparatus in non-rodent embryos

Citovat

POROKH, Volodymyr, Drahomira KYJOVSKA, Martina MARTONOVA, Tereza KLENKOVA, Pavel OTEVREL, Soňa KLOUDOVÁ a Zuzana HOLUBCOVÁ. Zygotic spindle orientation defines cleavage pattern and nuclear status of human embryos. Nature Communications. Berlin: Nature, 2024, roč. 15, č. 1, s. 1-9. ISSN 2041-1723. Dostupné z: https://dx.doi.org/10.1038/s41467-024-50732-z.

@article{2423145,
   author = {Porokh, Volodymyr and Kyjovska, Drahomira and Martonova, Martina and Klenkova, Tereza and Otevrel, Pavel and Kloudová, Soňa and Holubcová, Zuzana},
   article_location = {Berlin},
   article_number = {1},
   doi = {http://dx.doi.org/10.1038/s41467-024-50732-z},
   keywords = {zygotic spindle orientation; human embryos; cleavage pattern; nuclear status},
   language = {eng},
   issn = {2041-1723},
   journal = {Nature Communications},
   title = {Zygotic spindle orientation defines cleavage pattern and nuclear status of human embryos},
   url = {https://www.nature.com/articles/s41467-024-50732-z},
   volume = {15},
   year = {2024}
}

TY  - JOUR
ID  - 2423145
AU  - Porokh, Volodymyr - Kyjovska, Drahomira - Martonova, Martina - Klenkova, Tereza - Otevrel, Pavel - Kloudová, Soňa - Holubcová, Zuzana
PY  - 2024
TI  - Zygotic spindle orientation defines cleavage pattern and nuclear status of human embryos
JF  - Nature Communications
VL  - 15
IS  - 1
SP  - 1-9
EP  - 1-9
PB  - Nature
SN  - 20411723
KW  - zygotic spindle orientation
KW  - human embryos
KW  - cleavage pattern
KW  - nuclear status
UR  - https://www.nature.com/articles/s41467-024-50732-z
N2  - The first embryonic division represents a starting point for the development of a new individual. In many species, tight control over the first embryonic division ensures its accuracy. However, the first division in humans is often erroneous and can impair embryo development. To delineate the spatiotemporal organization of the first mitotic division typical for normal human embryo development, we systematically analyzed a unique timelapse dataset of 300 IVF embryos that developed into healthy newborns. The zygotic division pattern of these best-quality embryos was compared to their siblings that failed to implant or arrested during cleavage stage. We show that division at the right angle to the juxtaposed pronuclei is preferential and supports faithful zygotic division. Alternative configurations of the first mitosis are associated with reduced clustering of nucleoli and multinucleation at the 2-cell stage, which are more common in women of advanced age. Collectively, these data imply that orientation of the first division predisposes human embryos to genetic (in)stability and may contribute to aneuploidy and age-related infertility.
ER  -

POROKH, Volodymyr, Drahomira KYJOVSKA, Martina MARTONOVA, Tereza KLENKOVA, Pavel OTEVREL, Soňa KLOUDOVÁ a Zuzana HOLUBCOVÁ. Zygotic spindle orientation defines cleavage pattern and nuclear status of human embryos. \textit{Nature Communications}. Berlin: Nature, 2024, roč.~15, č.~1, s.~1-9. ISSN~2041-1723. Dostupné z: https://dx.doi.org/10.1038/s41467-024-50732-z.

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