Porcine model of a complicated skin and soft tissue infection
caused by Pseudomonas aeruginosa

J 2024

Porcine model of a complicated skin and soft tissue infection caused by Pseudomonas aeruginosa

LIPOVÝ, Břetislav, Lukáš VACEK, Dominika POLAŠTÍK KLEKNEROVÁ, Edita JEKLOVA, Lenka LIŠKOVÁ et. al.

Základní údaje

Originální název

Porcine model of a complicated skin and soft tissue infection caused by Pseudomonas aeruginosa

Autoři

LIPOVÝ, Břetislav (203 Česká republika, domácí), Lukáš VACEK (203 Česká republika, domácí), Dominika POLAŠTÍK KLEKNEROVÁ (703 Slovensko, domácí), Edita JEKLOVA (203 Česká republika), Lenka LIŠKOVÁ (203 Česká republika, domácí), Jakub HOLOUBEK (203 Česká republika, domácí), Dominika MATYSKOVÁ (203 Česká republika, domácí) a Filip RŮŽIČKA (203 Česká republika, domácí)

Vydání

Veterinarni Medicina, Prague, CZECH ACAD AGRIC SCI, 2024, 0375-8427

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10606 Microbiology

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 0.700 v roce 2022

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.17221/25/2024-VETMED

UT WoS

001259631600002

Klíčová slova anglicky

ESKAPE pathogen; model development; pig; wound

Štítky

14110113, 14110229, 14110517, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 16. 8. 2024 07:09, Mgr. Tereza Miškechová

Anotace

V originále

Pseudomonas aeruginosa poses a significant threat to both immunocompetent and immunocompromised individuals, often resulting in life -threatening infections. With increasing antimicrobial resistance, novel therapeutic strategies are urgently needed. Although animal models are crucial for preclinical studies, limited data are available for porcine models, more specifically for P. aeruginosa complicated skin and soft tissue infections (cSSTIs). This study presents a novel porcine model inducing and sustaining cSSTI for 14 days. Six pigs (120 wounds) were used for the development of infections, and within this group, two pigs (40 wounds) were used to evaluate the progression of the cSSTI infection. The model demonstrated bacterial loads of more than 10 7 CFU/ gram of tissue or higher. The cSSTI fully developed within three days and remained well above these levels until day 14 post -infection. Due to the immunocompetence of this model, all the immunological processes associated with the response to the presence of infection and the wound healing process are preserved.

Citovat

LIPOVÝ, Břetislav, Lukáš VACEK, Dominika POLAŠTÍK KLEKNEROVÁ, Edita JEKLOVA, Lenka LIŠKOVÁ, Jakub HOLOUBEK, Dominika MATYSKOVÁ a Filip RŮŽIČKA. Porcine model of a complicated skin and soft tissue infection caused by Pseudomonas aeruginosa. Veterinarni Medicina. Prague: CZECH ACAD AGRIC SCI, 2024, roč. 69, č. 6, s. 191-197. ISSN 0375-8427. Dostupné z: https://dx.doi.org/10.17221/25/2024-VETMED.

@article{2423258,
   author = {Lipový, Břetislav and Vacek, Lukáš and Polaštík Kleknerová, Dominika and Jeklova, Edita and Lišková, Lenka and Holoubek, Jakub and Matysková, Dominika and Růžička, Filip},
   article_location = {Prague},
   article_number = {6},
   doi = {http://dx.doi.org/10.17221/25/2024-VETMED},
   keywords = {ESKAPE pathogen; model development; pig; wound},
   language = {eng},
   issn = {0375-8427},
   journal = {Veterinarni Medicina},
   title = {Porcine model of a complicated skin and soft tissue infection caused by Pseudomonas aeruginosa},
   url = {https://vetmed.agriculturejournals.cz/artkey/vet-202406-0004_porcine-model-of-a-complicated-skin-and-soft-tissue-infection-caused-by-pseudomonas-aeruginosa.php},
   volume = {69},
   year = {2024}
}

TY  - JOUR
ID  - 2423258
AU  - Lipový, Břetislav - Vacek, Lukáš - Polaštík Kleknerová, Dominika - Jeklova, Edita - Lišková, Lenka - Holoubek, Jakub - Matysková, Dominika - Růžička, Filip
PY  - 2024
TI  - Porcine model of a complicated skin and soft tissue infection caused by Pseudomonas aeruginosa
JF  - Veterinarni Medicina
VL  - 69
IS  - 6
SP  - 191-197
EP  - 191-197
PB  - CZECH ACAD AGRIC SCI
SN  - 03758427
KW  - ESKAPE pathogen
KW  - model development
KW  - pig
KW  - wound
UR  - https://vetmed.agriculturejournals.cz/artkey/vet-202406-0004_porcine-model-of-a-complicated-skin-and-soft-tissue-infection-caused-by-pseudomonas-aeruginosa.php
N2  - Pseudomonas aeruginosa poses a significant threat to both immunocompetent and immunocompromised individuals, often resulting in life -threatening infections. With increasing antimicrobial resistance, novel therapeutic strategies are urgently needed. Although animal models are crucial for preclinical studies, limited data are available for porcine models, more specifically for P. aeruginosa complicated skin and soft tissue infections (cSSTIs). This study presents a novel porcine model inducing and sustaining cSSTI for 14 days. Six pigs (120 wounds) were used for the development of infections, and within this group, two pigs (40 wounds) were used to evaluate the progression of the cSSTI infection. The model demonstrated bacterial loads of more than 10 7 CFU/ gram of tissue or higher. The cSSTI fully developed within three days and remained well above these levels until day 14 post -infection. Due to the immunocompetence of this model, all the immunological processes associated with the response to the presence of infection and the wound healing process are preserved.
ER  -

LIPOVÝ, Břetislav, Lukáš VACEK, Dominika POLAŠTÍK KLEKNEROVÁ, Edita JEKLOVA, Lenka LIŠKOVÁ, Jakub HOLOUBEK, Dominika MATYSKOVÁ a Filip RŮŽIČKA. Porcine model of a complicated skin and soft tissue infection caused by Pseudomonas aeruginosa. \textit{Veterinarni Medicina}. Prague: CZECH ACAD AGRIC SCI, 2024, roč.~69, č.~6, s.~191-197. ISSN~0375-8427. Dostupné z: https://dx.doi.org/10.17221/25/2024-VETMED.

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