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@article{2423262,
author = {Zachova, K. and Bartheldyova, E. and Hubatka, F. and Krupka, M. and Odehnalova, N. and Turanek Knotigova, P. and Vaškovicová, Naděžda and Sloupenska, K. and Hromadka, R. and Paulovicova, E. and Effenberg, R. and Ledvinae, M. and Raska, M. and Turaneka, J.},
article_location = {London},
article_number = {May 2024},
doi = {http://dx.doi.org/10.1016/j.carbpol.2024.121844},
keywords = {Liposomes; Mannan; Recombinant protein; Adjuvants; Bioconjugation; Oxime ligation},
language = {eng},
issn = {0144-8617},
journal = {Carbohydrate Polymers},
title = {The immunogenicity of p24 protein from HIV-1 virus is strongly supported and modulated by coupling with liposomes and mannan},
url = {https://www.sciencedirect.com/science/article/pii/S0144861724000705?via%3Dihub},
volume = {332},
year = {2024}
}
TY - JOUR
ID - 2423262
AU - Zachova, K. - Bartheldyova, E. - Hubatka, F. - Krupka, M. - Odehnalova, N. - Turanek Knotigova, P. - Vaškovicová, Naděžda - Sloupenska, K. - Hromadka, R. - Paulovicova, E. - Effenberg, R. - Ledvinae, M. - Raska, M. - Turaneka, J.
PY - 2024
TI - The immunogenicity of p24 protein from HIV-1 virus is strongly supported and modulated by coupling with liposomes and mannan
JF - Carbohydrate Polymers
VL - 332
IS - May 2024
SP - 1-12
EP - 1-12
PB - ELSEVIER SCI LTD
SN - 01448617
KW - Liposomes
KW - Mannan
KW - Recombinant protein
KW - Adjuvants
KW - Bioconjugation
KW - Oxime ligation
UR - https://www.sciencedirect.com/science/article/pii/S0144861724000705?via%3Dihub
N2 - Anti-viral and anti-tumor vaccines aim to induce cytotoxic CD8+ T cells (CTL) and antibodies. Conserved protein antigens, such as p24 from human immunodeficiency virus, represent promising component for elicitation CTLs, nevertheless with suboptimal immunogenicity, if formulated as recombinant protein. To enhance immunogenicity and CTL response, recombinant proteins may be targeted to dendritic cells (DC) for cross presentation on MHCI, where mannose receptor and/or other lectin receptors could play an important role. Here, we constructed liposomal carrier-based vaccine composed of recombinant p24 antigen bound by metallochelating linkage onto surface of nanoliposomes with surface mannans coupled by aminooxy ligation. Generated mannosylated proteonanoliposomes were analyzed by dynamic light scattering, isothermal titration, and electron microscopy. Using murine DC line MutuDC and murine bone marrow derived DC (BMDC) we evaluated their immunogenicity and immunomodulatory activity. We show that p24 mannosylated proteonanoliposomes activate DC for enhanced MHCI, MHCII and CD40, CD80, and CD86 surface expression both on MutuDC and BMDC. p24 mannosylated liposomes were internalized by MutuDC with p24 intracellular localization within 1 to 3 h. The combination of metallochelating and aminooxy ligation could be used simultaneously to generate nanoliposomal adjuvanted recombinant protein-based vaccines versatile for combination of recombinant antigens relevant for antibody and CTL elicitation.
ER -
ZACHOVA, K., E. BARTHELDYOVA, F. HUBATKA, M. KRUPKA, N. ODEHNALOVA, P. TURANEK KNOTIGOVA, Naděžda VAŠKOVICOVÁ, K. SLOUPENSKA, R. HROMADKA, E. PAULOVICOVA, R. EFFENBERG, M. LEDVINAE, M. RASKA a J. TURANEKA. The immunogenicity of p24 protein from HIV-1 virus is strongly supported and modulated by coupling with liposomes and mannan. \textit{Carbohydrate Polymers}. London: ELSEVIER SCI LTD, 2024, roč.~332, May 2024, s.~1-12. ISSN~0144-8617. Dostupné z: https://dx.doi.org/10.1016/j.carbpol.2024.121844.
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