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@proceedings{2431822,
author = {Hassan, Ahmed Adel Ibrahim Hassona and Pinkas, Matyáš and Ito, Kosuke and Uchiumi, Toshio and Demo, Gabriel},
booktitle = {RNA society meeting 2024},
keywords = {ribosome subunit dimerization cryoEM structure},
language = {eng},
title = {A novel archaeal ribosome anti-association factor promoting a distinctive 30S-30S dimerization},
url = {https://www2.rnasociety.org/},
year = {2024}
}
TY - CONF
ID - 2431822
AU - Hassan, Ahmed Adel Ibrahim Hassona - Pinkas, Matyáš - Ito, Kosuke - Uchiumi, Toshio - Demo, Gabriel
PY - 2024
TI - A novel archaeal ribosome anti-association factor promoting a distinctive 30S-30S dimerization
KW - ribosome subunit dimerization cryoEM structure
UR - https://www2.rnasociety.org/
N2 - Protein synthesis consumes a substantial portion of cellular resources, prompting specialized mechanisms to reduce the translation during adverse conditions. Ribosome inactivation often involves ribosome-interacting proteins. During stationary phase, ribosome modulation factor (RMF) and hibernation-promoting factor (HPF) in some γ-proteobacteria initiate the formation of translationally inactive 100S ribosome dimers. Additionally, bacteria utilize ribosome silencing or anti-association factors to prevent active 70S ribosome formation. Despite extensive studies in bacteria, insights into factor-mediated ribosome dimerization or anti-association in archaea remain elusive. Here, we present the first cryo-electron microscopy structures of archaeal 30S dimer complexed with a novel archaeal ribosome dimerization factor (termed as aRDF) from Pyrococcus furiosus. The complex exhibits two 30S subunits stabilized by aRDF homodimers in a head-to-body architecture. aRDF interacts directly with the L41e ribosomal protein, essential for subunit association. The binding mode of aRDF demonstrates its anti-association ability, preventing the assembly of archaeal 70S ribosomes.
ER -
HASSAN, Ahmed Adel Ibrahim Hassona, Matyáš PINKAS, Kosuke ITO, Toshio UCHIUMI a Gabriel DEMO. A novel archaeal ribosome anti-association factor promoting a distinctive 30S-30S dimerization. In \textit{RNA society meeting 2024}. 2024.
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