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@article{2431897,
author = {Navrátil, Jiří and Kratochvílová, Monika and Raudenská, Martina and Balvan, Jan and Vičar, Tomáš and Petrláková, Kateřina and Suzuki, Kanako and Jadrna, Lucie and Bursa, Jiri and Kräter, Martin and Kim, Kyoohyun and Masařík, Michal and Gumulec, Jaromír},
article_location = {London},
doi = {http://dx.doi.org/10.1186/s12935-024-03495-y},
keywords = {Mechanobiology; Zinc; Actin; Cytoskeleton; Mitochondria; Vimentin; Metabolism; Cancer},
language = {eng},
issn = {1475-2867},
journal = {Cancer Cell International},
title = {Long-term zinc treatment alters the mechanical properties and metabolism of prostate cancer cells},
url = {https://cancerci.biomedcentral.com/articles/10.1186/s12935-024-03495-y},
year = {2024}
}
TY - JOUR
ID - 2431897
AU - Navrátil, Jiří - Kratochvílová, Monika - Raudenská, Martina - Balvan, Jan - Vičar, Tomáš - Petrláková, Kateřina - Suzuki, Kanako - Jadrna, Lucie - Bursa, Jiri - Kräter, Martin - Kim, Kyoohyun - Masařík, Michal - Gumulec, Jaromír
PY - 2024
TI - Long-term zinc treatment alters the mechanical properties and metabolism of prostate cancer cells
JF - Cancer Cell International
PB - BMC
SN - 14752867
KW - Mechanobiology
KW - Zinc
KW - Actin
KW - Cytoskeleton
KW - Mitochondria
KW - Vimentin
KW - Metabolism
KW - Cancer
UR - https://cancerci.biomedcentral.com/articles/10.1186/s12935-024-03495-y
N2 - The failure of intracellular zinc accumulation is a key process in prostate carcinogenesis. Although prostate cancer cells can accumulate zinc after long-term exposure, chronic zinc oversupply may accelerate prostate carcinogenesis or chemoresistance. Because cancer progression is associated with energetically demanding cytoskeletal rearrangements, we investigated the effect of long-term zinc presence on biophysical parameters, ATP production, and EMT characteristics of two prostate cancer cell lines (PC-3, 22Rv1). Prolonged exposure to zinc increased ATP production, spare respiratory capacity, and induced a response in PC-3 cells, characterized by remodeling of vimentin and a shift of cell dry mass density and caveolin-1 to the perinuclear region. This zinc-induced remodeling correlated with a greater tendency to maintain actin architecture despite inhibition of actin polymerization by cytochalasin. Zinc partially restored epithelial characteristics in PC-3 cells by decreasing vimentin expression and increasing E-cadherin. Nevertheless, the expression of E-cadherin remained lower than that observed in predominantly oxidative, low-invasive 22Rv1 cells. Following long-term zinc exposure, we observed an increase in cell stiffness associated with an increased refractive index in the perinuclear region and an increased mitochondrial content. The findings of the computational simulations indicate that the mechanical response cannot be attributed exclusively to alterations in cytoskeletal composition. This observation suggests the potential involvement of an additional, as yet unidentified, mechanical contributor. These findings indicate that long-term zinc exposure alters a group of cellular parameters towards an invasive phenotype, including an increase in mitochondrial number, ATP production, and cytochalasin resistance. Ultimately, these alterations are manifested in the biomechanical properties of the cells.
ER -
NAVRÁTIL, Jiří, Monika KRATOCHVÍLOVÁ, Martina RAUDENSKÁ, Jan BALVAN, Tomáš VIČAR, Kateřina PETRLÁKOVÁ, Kanako SUZUKI, Lucie JADRNA, Jiri BURSA, Martin KRÄTER, Kyoohyun KIM, Michal MASAŘÍK a Jaromír GUMULEC. Long-term zinc treatment alters the mechanical properties and metabolism of prostate cancer cells. \textit{Cancer Cell International}. London: BMC, 2024. ISSN~1475-2867. Dostupné z: https://dx.doi.org/10.1186/s12935-024-03495-y.
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