Dual Nature of MoO3 Particles in Human-Derived Macrophage
Milieu

J 2024

Dual Nature of MoO3 Particles in Human-Derived Macrophage Milieu

NAVRÁTILOVÁ, Polina; Daniel WOJTAS; Anna WÓJCIK; Sanjay Gopal ULLATTIL; Tomáš LOJA et. al.

Základní údaje

Originální název

Dual Nature of MoO3 Particles in Human-Derived Macrophage Milieu

Autoři

NAVRÁTILOVÁ, Polina (643 Rusko, domácí); Daniel WOJTAS (616 Polsko, domácí); Anna WÓJCIK (616 Polsko); Sanjay Gopal ULLATTIL; Tomáš LOJA (703 Slovensko, domácí) a Monika PÁVKOVÁ GOLDBERGOVÁ (203 Česká republika, domácí)

Vydání

ACS Applied Nano Materials, Washington, American Chemical Society, 2024, 2574-0970

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10609 Biochemical research methods

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 5.500

Kód RIV

RIV/00216224:14110/24:00137112

Organizační jednotka

Lékařská fakulta

DOI

https://doi.org/10.1021/acsanm.4c03385

UT WoS

001310564400001

EID Scopus

2-s2.0-85203816845

Klíčová slova anglicky

MoO3; macrophages; cytotoxicity; reactive oxygen species; oxidative stress

Štítky

14110518, CF CELLIM, podil, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 6. 6. 2025 12:22, Mgr. Tereza Miškechová

Anotace

V originále

Molybdenum trioxide (MoO3) has over the years captivated significant scientific attention, mainly in infection prevention and cancer treatment. However, as cytotoxicity of the compound poses a great concern, herein we test the hypothesis that MoO3 in the form of commercially available particles triggers cytotoxic responses yet may also act as an antioxidant. In doing so, human-derived macrophages were exposed to variously concentrated (50–500 μM) MoO3 particle suspensions and examined from the cytocompatibility point of view. Cytotoxicity assays were coupled with cell morphology characterization via confocal laser scanning microscopy imaging, reactive oxygen species (ROS) detection, and flow cytometry for macrophage polarization analysis. Moreover, the DPPH assay for radical scavenging ability was performed. Generally, dose-dependent cytotoxicity of MoO3 was noted, with concentrations up to 100 μM being well-tolerated by macrophages. The particles demonstrated antioxidant activity, as confirmed by their ability to scavenge DPPH radicals. However, at the highest concentration studied, i.e., 500 μM, MoO3 became highly cytotoxic and triggered cell death, as evidenced by alterations in cell shape and abnormal ROS production. Overall, the antioxidative and cytotoxic character of MoO3 particles at low and high concentrations, respectively, was highlighted. The findings pave the way for future studies in the area of MoO3-based materials and validate their usefulness for biomedical purposes, as in antioxidant-based treatments.

Návaznosti

NU20-08-00149, projekt VaV

Název: Multicentrické hodnocení hypersenzitivní reakce u pacientů indikovaných k totální náhradě kloubu včetně hodnocení důvodů reimplanace

Investor: Ministerstvo zdravotnictví ČR, Multicentrické hodnocení hypersenzitivní reakce u pacientů indikovaných k totální náhradě kloubu včetně hodnocení důvodů reimplanace, Podprogram 1 - standardní

90250, velká výzkumná infrastruktura

Název: Czech-BioImaging III

90251, velká výzkumná infrastruktura

Název: CzechNanoLab II

Citovat

NAVRÁTILOVÁ, Polina; Daniel WOJTAS; Anna WÓJCIK; Sanjay Gopal ULLATTIL; Tomáš LOJA a Monika PÁVKOVÁ GOLDBERGOVÁ. Dual Nature of MoO3 Particles in Human-Derived Macrophage Milieu. ACS Applied Nano Materials. Washington: American Chemical Society, 2024, roč. 7, č. 18, s. 21495-21506. ISSN 2574-0970. Dostupné z: https://doi.org/10.1021/acsanm.4c03385.

@article{2433137,
   author = {Navrátilová, Polina and Wojtas, Daniel and Wójcik, Anna and Ullattil, Sanjay Gopal and Loja, Tomáš and Pávková Goldbergová, Monika},
   article_location = {Washington},
   article_number = {18},
   doi = {https://doi.org/10.1021/acsanm.4c03385},
   keywords = {MoO3; macrophages; cytotoxicity; reactive oxygen species; oxidative stress},
   language = {eng},
   issn = {2574-0970},
   journal = {ACS Applied Nano Materials},
   title = {Dual Nature of MoO3 Particles in Human-Derived Macrophage Milieu},
   url = {https://pubs.acs.org/doi/full/10.1021/acsanm.4c03385},
   volume = {7},
   year = {2024}
}

TY  - JOUR
ID  - 2433137
AU  - Navrátilová, Polina - Wojtas, Daniel - Wójcik, Anna - Ullattil, Sanjay Gopal - Loja, Tomáš - Pávková Goldbergová, Monika
PY  - 2024
TI  - Dual Nature of MoO3 Particles in Human-Derived Macrophage Milieu
JF  - ACS Applied Nano Materials
VL  - 7
IS  - 18
SP  - 21495-21506
EP  - 21495-21506
PB  - American Chemical Society
SN  - 25740970
KW  - MoO3
KW  - macrophages
KW  - cytotoxicity
KW  - reactive oxygen species
KW  - oxidative stress
UR  - https://pubs.acs.org/doi/full/10.1021/acsanm.4c03385
N2  - Molybdenum trioxide (MoO3) has over the years captivated significant scientific attention, mainly in infection prevention and cancer treatment. However, as cytotoxicity of the compound poses a great concern, herein we test the hypothesis that MoO3 in the form of commercially available particles triggers cytotoxic responses yet may also act as an antioxidant. In doing so, human-derived macrophages were exposed to variously concentrated (50–500 μM) MoO3 particle suspensions and examined from the cytocompatibility point of view. Cytotoxicity assays were coupled with cell morphology characterization via confocal laser scanning microscopy imaging, reactive oxygen species (ROS) detection, and flow cytometry for macrophage polarization analysis. Moreover, the DPPH assay for radical scavenging ability was performed. Generally, dose-dependent cytotoxicity of MoO3 was noted, with concentrations up to 100 μM being well-tolerated by macrophages. The particles demonstrated antioxidant activity, as confirmed by their ability to scavenge DPPH radicals. However, at the highest concentration studied, i.e., 500 μM, MoO3 became highly cytotoxic and triggered cell death, as evidenced by alterations in cell shape and abnormal ROS production. Overall, the antioxidative and cytotoxic character of MoO3 particles at low and high concentrations, respectively, was highlighted. The findings pave the way for future studies in the area of MoO3-based materials and validate their usefulness for biomedical purposes, as in antioxidant-based treatments.
ER  -

NAVRÁTILOVÁ, Polina; Daniel WOJTAS; Anna WÓJCIK; Sanjay Gopal ULLATTIL; Tomáš LOJA a Monika PÁVKOVÁ GOLDBERGOVÁ. Dual Nature of MoO3 Particles in Human-Derived Macrophage Milieu. \textit{ACS Applied Nano Materials}. Washington: American Chemical Society, 2024, roč.~7, č.~18, s.~21495-21506. ISSN~2574-0970. Dostupné z: https://doi.org/10.1021/acsanm.4c03385.

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