Histone Chaperone Deficiency in Arabidopsis Plants Triggers
Adaptive Epigenetic Changes in Histone Variants and
Modifications

J 2024

Histone Chaperone Deficiency in Arabidopsis Plants Triggers Adaptive Epigenetic Changes in Histone Variants and Modifications

FRANEK, Michal; Martina NEŠPOR DADEJOVÁ; Pavlína PÍREK; Karolína KRYŠTOFOVÁ; Tereza DOBISOVÁ et. al.

Základní údaje

Originální název

Histone Chaperone Deficiency in Arabidopsis Plants Triggers Adaptive Epigenetic Changes in Histone Variants and Modifications

Autoři

FRANEK, Michal (703 Slovensko, domácí); Martina NEŠPOR DADEJOVÁ (203 Česká republika, domácí); Pavlína PÍREK (203 Česká republika, domácí); Karolína KRYŠTOFOVÁ (203 Česká republika, domácí); Tereza DOBISOVÁ (203 Česká republika); Zbyněk ZDRÁHAL (203 Česká republika, domácí); Martina DVOŘÁČKOVÁ (203 Česká republika, domácí) a Gabriela LOCHMANOVÁ (203 Česká republika, domácí)

Vydání

MOLECULAR & CELLULAR PROTEOMICS, ELSEVIERRADARWEG 29, 1043 NX AMSTERDAM, ELSEVIER, 2024, 1535-9484

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10609 Biochemical research methods

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Kód RIV

RIV/00216224:14740/24:00137145

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1016/j.mcpro.2024.100795

UT WoS

001347418600001

EID Scopus

2-s2.0-85199934115

Klíčová slova anglicky

Arabidopsis; chromatin remodeling; histone chaperone complex; histone variants; immunochemistry; mass spectrometry; post-translational modifications

Štítky

CF CELLIM, CF PLANT, CF PROT, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 3. 6. 2025 13:26, Mgr. Eva Dubská

Anotace

V originále

At the molecular scale, adaptive advantages during plant growth and development rely on modulation of gene expression, primarily provided by epigenetic machinery. One crucial part of this machinery is histone posttranslational modifications, which form a flexible system, driving transient changes in chromatin, and defining particular epigenetic states. Posttranslational modifications work in concert with replication-independent histone variants further adapted for transcriptional regulation and chromatin repair. However, little is known about how such complex regulatory pathways are orchestrated and interconnected in cells. In this work, we demonstrate the utility of mass spectrometry-based approaches to explore how different epigenetic layers interact in Arabidopsis mutants lacking certain histone chaperones. We show that defects in histone chaperone function (e.g., chromatin assembly factor-1 or nucleosome assembly protein 1 mutations) translate into an altered epigenetic landscape, which aids the plant in mitigating internal instability. We observe changes in both the levels and distribution of H2A.W.7, altogether with partial repurposing of H3.3 and changes in the key repressive (H3K27me1/2) or euchromatic marks (H3K36me1/2). These shifts in the epigenetic profile serve as a compensatory mechanism in response to impaired integration of the H3.1 histone in the fas1 mutants. Altogether, our findings suggest that maintaining genome stability involves a two-tiered approach. The first relies on flexible adjustments in histone marks, while the second level requires the assistance of chaperones for histone variant replacement.

Návaznosti

EF16_026/0008446, projekt VaV

Název: Integrace signálu a epigenetické reprogramování pro produktivitu rostlin

GA22-28190S, projekt VaV

Název: Komplexní charakterizace histonových epigenetických značek

Investor: Grantová agentura ČR, Komplexní charakterizace histonových epigenetických značek

GA23-06643S, projekt VaV

Název: Dispozice, výskyt a zpracování DNA lézí v genomu rostlin Arabidopsis thaliana defektních pro reparaci DNA a sestavování nukleozomů

Investor: Grantová agentura ČR, Dispozice, výskyt a zpracování DNA lézí v genomu rostlin Arabidopsis thaliana defektních pro reparaci DNA a sestavování nukleozomů

MUNI/R/1364/2023, interní kód MU

Název: Mechanizmy opravy DNA u rostliny Arabidopsis thaliana

Investor: Masarykova univerzita, Mechanizmy opravy DNA u rostliny Arabidopsis thaliana, CAREER RESTART

90242, velká výzkumná infrastruktura

Název: CIISB III

90254, velká výzkumná infrastruktura

Název: e-INFRA CZ II

Citovat

FRANEK, Michal; Martina NEŠPOR DADEJOVÁ; Pavlína PÍREK; Karolína KRYŠTOFOVÁ; Tereza DOBISOVÁ; Zbyněk ZDRÁHAL; Martina DVOŘÁČKOVÁ a Gabriela LOCHMANOVÁ. Histone Chaperone Deficiency in Arabidopsis Plants Triggers Adaptive Epigenetic Changes in Histone Variants and Modifications. MOLECULAR & CELLULAR PROTEOMICS. ELSEVIERRADARWEG 29, 1043 NX AMSTERDAM,: ELSEVIER, 2024, roč. 23, č. 7, s. 1-17. ISSN 1535-9484. Dostupné z: https://dx.doi.org/10.1016/j.mcpro.2024.100795.

@article{2433977,
   author = {Franek, Michal and Nešpor Dadejová, Martina and Pírek, Pavlína and Kryštofová, Karolína and Dobisová, Tereza and Zdráhal, Zbyněk and Dvořáčková, Martina and Lochmanová, Gabriela},
   article_location = {ELSEVIERRADARWEG 29, 1043 NX AMSTERDAM,},
   article_number = {7},
   doi = {http://dx.doi.org/10.1016/j.mcpro.2024.100795},
   keywords = {Arabidopsis; chromatin remodeling; histone chaperone complex; histone variants; immunochemistry; mass spectrometry; post-translational modifications},
   language = {eng},
   issn = {1535-9484},
   journal = {MOLECULAR & CELLULAR PROTEOMICS},
   title = {Histone Chaperone Deficiency in Arabidopsis Plants Triggers Adaptive Epigenetic Changes in Histone Variants and Modifications},
   url = {https://www.mcponline.org/article/S1535-9476(24)00085-9/fulltext},
   volume = {23},
   year = {2024}
}

TY  - JOUR
ID  - 2433977
AU  - Franek, Michal - Nešpor Dadejová, Martina - Pírek, Pavlína - Kryštofová, Karolína - Dobisová, Tereza - Zdráhal, Zbyněk - Dvořáčková, Martina - Lochmanová, Gabriela
PY  - 2024
TI  - Histone Chaperone Deficiency in Arabidopsis Plants Triggers Adaptive Epigenetic Changes in Histone Variants and Modifications
JF  - MOLECULAR & CELLULAR PROTEOMICS
VL  - 23
IS  - 7
SP  - 1-17
EP  - 1-17
PB  - ELSEVIER
SN  - 15359484
KW  - Arabidopsis
KW  - chromatin remodeling
KW  - histone chaperone complex
KW  - histone variants
KW  - immunochemistry
KW  - mass spectrometry
KW  - post-translational modifications
UR  - https://www.mcponline.org/article/S1535-9476(24)00085-9/fulltext
N2  - At the molecular scale, adaptive advantages during plant growth and development rely on modulation of gene expression, primarily provided by epigenetic machinery. One crucial part of this machinery is histone posttranslational modifications, which form a flexible system, driving transient changes in chromatin, and defining particular epigenetic states. Posttranslational modifications work in concert with replication-independent histone variants further adapted for transcriptional regulation and chromatin repair. However, little is known about how such complex regulatory pathways are orchestrated and interconnected in cells. In this work, we demonstrate the utility of mass spectrometry-based approaches to explore how different epigenetic layers interact in Arabidopsis mutants lacking certain histone chaperones. We show that defects in histone chaperone function (e.g., chromatin assembly factor-1 or nucleosome assembly protein 1 mutations) translate into an altered epigenetic landscape, which aids the plant in mitigating internal instability. We observe changes in both the levels and distribution of H2A.W.7, altogether with partial repurposing of H3.3 and changes in the key repressive (H3K27me1/2) or euchromatic marks (H3K36me1/2). These shifts in the epigenetic profile serve as a compensatory mechanism in response to impaired integration of the H3.1 histone in the fas1 mutants. Altogether, our findings suggest that maintaining genome stability involves a two-tiered approach. The first relies on flexible adjustments in histone marks, while the second level requires the assistance of chaperones for histone variant replacement.
ER  -

FRANEK, Michal; Martina NEŠPOR DADEJOVÁ; Pavlína PÍREK; Karolína KRYŠTOFOVÁ; Tereza DOBISOVÁ; Zbyněk ZDRÁHAL; Martina DVOŘÁČKOVÁ a Gabriela LOCHMANOVÁ. Histone Chaperone Deficiency in Arabidopsis Plants Triggers Adaptive Epigenetic Changes in Histone Variants and Modifications. \textit{MOLECULAR \&{} CELLULAR PROTEOMICS}. ELSEVIERRADARWEG 29, 1043 NX AMSTERDAM,: ELSEVIER, 2024, roč.~23, č.~7, s.~1-17. ISSN~1535-9484. Dostupné z: https://dx.doi.org/10.1016/j.mcpro.2024.100795.

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