Detailed Information on Publication Record
2000
Determination of glutathione by micellar electrokinetic chromatography and on-column reaction with 2,2-dipyridyldisulphide
GLATZ, Zdeněk and Hana MAŠLAŇOVÁBasic information
Original name
Determination of glutathione by micellar electrokinetic chromatography and on-column reaction with 2,2-dipyridyldisulphide
Authors
GLATZ, Zdeněk and Hana MAŠLAŇOVÁ
Edition
Saarbrucken, Germany, Book of Abstract "13th International symposium on high performance capillary electrophoresis and related techniques HPCE 2000". p. 155-156, 2000
Publisher
Universites des Saarlandes
Other information
Language
English
Type of outcome
Stať ve sborníku
Field of Study
10600 1.6 Biological sciences
Country of publisher
Germany
Confidentiality degree
není předmětem státního či obchodního tajemství
RIV identification code
RIV/00216224:14310/00:00003265
Organization unit
Faculty of Science
Keywords in English
glutathione; on-column derivatization; MEKC
Změněno: 27/2/2001 15:56, prof. RNDr. Zdeněk Glatz, CSc.
Abstract
V originále
A new method for specific determination of glutathione using micellar electrokinetic chromatography and on-column reaction with 2,2 -dipyridyldisulphide is described. 2,2-dipyridyldisulphide and a sample of glutathione are injected consecutively into the capillary as two discrete plugs separated with a short plug of background electrolyte. Due to the differences in the mobilities of the 2,2-dipyridyldisulphide and glutathione, on-column mixing and reaction occur. Glutathione is in this reaction quantitatively transformed into a mixed disulphide concomitantly with formation of equimolar amount of the 2-thiopyridone which is further separated by micellar electrokinetic chromatography and determined spectrophotometrically at 343 nm. The concentration of glutathione is thus estimated indirectly from the result of 2-thiopyridone determination.
Links
| GA525/00/0785, research and development project |
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