Optimization of saliva sampling methods for analysis of bile
acids by UHPLC-MS

J 2024

Optimization of saliva sampling methods for analysis of bile acids by UHPLC-MS

DOSEDELOVA, Vera, Marketa LASTOVICKOVA, Štefan KONEČNÝ, Jiří DOLINA, Petr KUBAN et. al.

Základní údaje

Originální název

Optimization of saliva sampling methods for analysis of bile acids by UHPLC-MS

Autoři

DOSEDELOVA, Vera (203 Česká republika), Marketa LASTOVICKOVA (203 Česká republika), Štefan KONEČNÝ (703 Slovensko, domácí), Jiří DOLINA (203 Česká republika, domácí) a Petr KUBAN (203 Česká republika)

Vydání

Journal of Chromatography, AMSTERDAM, Elsevier, 2024, 0021-9673

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30219 Gastroenterology and hepatology

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.100 v roce 2022

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1016/j.chroma.2024.465354

UT WoS

001316072100001

Klíčová slova anglicky

Barrett's esophagus; Bile acids; Reflux disease; Saliva; Saliva sampling methods

Štítky

14110213, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 1. 10. 2024 10:18, Mgr. Tereza Miškechová

Anotace

V originále

This study investigated methods for sampling bile acids in saliva, a potential non-invasive diagnostic biofluid. Bile acids have been implicated in causing damage and permanent changes to the esophageal mucosa and increasing the risk of developing Barrett's esophagus, a condition that can potentially progress to esophageal cancer. Three saliva collection methods were compared: spitting, Salivette (R) swabs, and Salivette Cortisol (R) swabs. Spitting emerged as the superior method with the highest recoveries and the least interference, likely due to Salivette swabs retaining bile acids or introducing unknown interferences. All saliva samples were analyzed by UHPLC-MS/MS using the Zorbax RRHD Eclipse Plus C18 column (3 x 50 mm, 1.8 mu m) in gradient elution of 0.1 % formic acid in water and methanol. Saliva sample stability was assessed over 14 days, reflecting typical storage times. The levels of detected bile acids were stable for the measured period (RSD <= 22 %) and no degradation was observed. Bile acid levels in saliva fluctuated throughout the day, with the greatest changes observed for glycine-conjugated bile acids after meals. To minimize sampling variability, saliva collection by spitting after overnight fasting is recommended for future studies. Our findings are applicable for standardized bile acid sampling and are currently applied in a large clinical study evaluating bile acids as potential susceptibility markers for Barrett's esophagus diagnostics.

Citovat

DOSEDELOVA, Vera, Marketa LASTOVICKOVA, Štefan KONEČNÝ, Jiří DOLINA a Petr KUBAN. Optimization of saliva sampling methods for analysis of bile acids by UHPLC-MS. Journal of Chromatography. AMSTERDAM: Elsevier, 2024, roč. 1736, November 2024, s. 1-7. ISSN 0021-9673. Dostupné z: https://dx.doi.org/10.1016/j.chroma.2024.465354.

@article{2439064,
   author = {Dosedelova, Vera and Lastovickova, Marketa and Konečný, Štefan and Dolina, Jiří and Kuban, Petr},
   article_location = {AMSTERDAM},
   article_number = {November 2024},
   doi = {http://dx.doi.org/10.1016/j.chroma.2024.465354},
   keywords = {Barrett's esophagus; Bile acids; Reflux disease; Saliva; Saliva sampling methods},
   language = {eng},
   issn = {0021-9673},
   journal = {Journal of Chromatography},
   title = {Optimization of saliva sampling methods for analysis of bile acids by UHPLC-MS},
   url = {https://www.sciencedirect.com/science/article/pii/S0021967324007283?via%3Dihub},
   volume = {1736},
   year = {2024}
}

TY  - JOUR
ID  - 2439064
AU  - Dosedelova, Vera - Lastovickova, Marketa - Konečný, Štefan - Dolina, Jiří - Kuban, Petr
PY  - 2024
TI  - Optimization of saliva sampling methods for analysis of bile acids by UHPLC-MS
JF  - Journal of Chromatography
VL  - 1736
IS  - November 2024
SP  - 1-7
EP  - 1-7
PB  - Elsevier
SN  - 00219673
KW  - Barrett's esophagus
KW  - Bile acids
KW  - Reflux disease
KW  - Saliva
KW  - Saliva sampling methods
UR  - https://www.sciencedirect.com/science/article/pii/S0021967324007283?via%3Dihub
N2  - This study investigated methods for sampling bile acids in saliva, a potential non-invasive diagnostic biofluid. Bile acids have been implicated in causing damage and permanent changes to the esophageal mucosa and increasing the risk of developing Barrett's esophagus, a condition that can potentially progress to esophageal cancer. Three saliva collection methods were compared: spitting, Salivette (R) swabs, and Salivette Cortisol (R) swabs. Spitting emerged as the superior method with the highest recoveries and the least interference, likely due to Salivette swabs retaining bile acids or introducing unknown interferences. All saliva samples were analyzed by UHPLC-MS/MS using the Zorbax RRHD Eclipse Plus C18 column (3 x 50 mm, 1.8 mu m) in gradient elution of 0.1 % formic acid in water and methanol. Saliva sample stability was assessed over 14 days, reflecting typical storage times. The levels of detected bile acids were stable for the measured period (RSD <= 22 %) and no degradation was observed. Bile acid levels in saliva fluctuated throughout the day, with the greatest changes observed for glycine-conjugated bile acids after meals. To minimize sampling variability, saliva collection by spitting after overnight fasting is recommended for future studies. Our findings are applicable for standardized bile acid sampling and are currently applied in a large clinical study evaluating bile acids as potential susceptibility markers for Barrett's esophagus diagnostics.
ER  -

DOSEDELOVA, Vera, Marketa LASTOVICKOVA, Štefan KONEČNÝ, Jiří DOLINA a Petr KUBAN. Optimization of saliva sampling methods for analysis of bile acids by UHPLC-MS. \textit{Journal of Chromatography}. AMSTERDAM: Elsevier, 2024, roč.~1736, November 2024, s.~1-7. ISSN~0021-9673. Dostupné z: https://dx.doi.org/10.1016/j.chroma.2024.465354.

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