Determination of thiocyanate in human plasma and urine.

D 2000

Determination of thiocyanate in human plasma and urine.

NOVÁKOVÁ, Soňa, Hana ŠTĚRBOVÁ a Zdeněk GLATZ

Základní údaje

Originální název

Determination of thiocyanate in human plasma and urine.

Autoři

NOVÁKOVÁ, Soňa, Hana ŠTĚRBOVÁ a Zdeněk GLATZ

Vydání

první. Brno, Biochemie a molekulární biologie na prahu nového tisíciletí, s. 57-57, 2000

Nakladatel

MU, Brno

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14310/00:00003269

Organizační jednotka

Přírodovědecká fakulta

ISBN

80-210-2266-3

Klíčová slova anglicky

thiokyanat; kapilární zonová elektroforéza;

Štítky

kapilární zonová elektroforéza, thiokyanat

Změněno: 27. 2. 2001 10:36, prof. RNDr. Zdeněk Glatz, CSc.

Anotace

V originále

The thiocyanate ion is usually present in low concentration in human urine, serum and saliva as a result of the digestion of some vegetables containing glucosinolates. Higher concentrations of this ion, that is a metabolic product of cyanide, arise from tobacco smoke. The level of thiocyanate is thus considered as a good probe for distinguishing between smokers and non-smokers. Moreover thiocyanate is also known to block iodine uptake by the thyroid gland. Several methods have been reported for the determination of this ion in biological samples. These are the spectrophotometric or flow injection methods based on the reaction with Fe3+ or on the König reaction, gas chromatography, ion chromatography and atomic absorption spectrophotometry. Many of these methods are complicated, laborious to perform and required harmful reagents. A new sensitive and simple method has been developed for the determination of thiocyanate in human plasma and urine. The determinations were performed in a 75 mm fused silica capillary using 0.1 M b-alanine - HCl (pH 3.50) as a background electrolyte, separation voltage 18 kV (negative polarity), 25 oC temperature of capillary and direct detection at 200 nm. Plasma samples were 10-times diluted with deionesed water and deproteinized with acetonitrile in the ratio 1 : 2. Urine samples need only 20-fold dilution with deionesed water. The proposed method was successfully applied to the determination of thiocyanate in various human plasma and urine samples.

Návaznosti

GA525/00/0785, projekt VaV

Název: Acidifikační procesy v sulfidových odpadech

Investor: Grantová agentura ČR, Acidifikační procesy v sulfidových odpadech

Citovat

NOVÁKOVÁ, Soňa, Hana ŠTĚRBOVÁ a Zdeněk GLATZ. Determination of thiocyanate in human plasma and urine. In Biochemie a molekulární biologie na prahu nového tisíciletí. první. Brno: MU, Brno, 2000, s. 57. ISBN 80-210-2266-3.

@inproceedings{245353,
   author = {Nováková, Soňa and Štěrbová, Hana and Glatz, Zdeněk},
   address = {Brno},
   booktitle = {Biochemie a molekulární biologie na prahu nového tisíciletí},
   edition = {první},
   keywords = {thiokyanat; kapilární zonová elektroforéza;},
   language = {eng},
   location = {Brno},
   isbn = {80-210-2266-3},
   pages = {57-57},
   publisher = {MU, Brno},
   title = {Determination of thiocyanate in human plasma and urine.},
   year = {2000}
}

TY  - JOUR
ID  - 245353
AU  - Nováková, Soňa - Štěrbová, Hana - Glatz, Zdeněk
PY  - 2000
TI  - Determination of thiocyanate in human plasma and urine.
PB  - MU, Brno
CY  - Brno
SN  - 8021022663
KW  - thiokyanat
KW  - kapilární zonová elektroforéza;
N2  - The thiocyanate ion is usually present in low concentration in human urine, serum and saliva as a result of the digestion of some vegetables containing glucosinolates. Higher concentrations of this ion, that is a metabolic product of cyanide, arise from tobacco smoke. The level of thiocyanate is thus considered as a good probe for distinguishing between smokers and non-smokers. Moreover thiocyanate is also known to block iodine uptake by the thyroid gland. Several methods have been reported for the determination of this ion in biological samples. These are the spectrophotometric or flow injection methods based on the reaction with Fe3+ or on the König reaction, gas chromatography, ion chromatography and atomic absorption spectrophotometry. Many of these methods are complicated, laborious to perform and required harmful reagents. A new sensitive and simple method has been developed for the determination of thiocyanate in human plasma and urine. The determinations were performed in a 75 mm fused silica capillary using 0.1 M b-alanine - HCl (pH 3.50) as a background electrolyte, separation voltage 18 kV (negative polarity), 25 oC temperature of capillary and direct detection at 200 nm. Plasma samples were 10-times diluted with deionesed water and deproteinized with acetonitrile in the ratio 1 : 2. Urine samples need only 20-fold dilution with deionesed water. The proposed method was successfully applied to the determination of thiocyanate in various human plasma and urine samples.
ER  -

NOVÁKOVÁ, Soňa, Hana ŠTĚRBOVÁ a Zdeněk GLATZ. Determination of thiocyanate in human plasma and urine. In \textit{Biochemie a molekulární biologie na prahu nového tisíciletí}. první. Brno: MU, Brno, 2000, s.~57. ISBN~80-210-2266-3.

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