Linking planar polarity signalling to actomyosin contractility
during vertebrate neurulation

J 2024

Linking planar polarity signalling to actomyosin contractility during vertebrate neurulation

NOVOTNÁ, Šárka; Lorena AGOSTINI MAIA; Katarzyna Anna RADASZKIEWICZ; Pavel ROUDNICKÝ; Jakub HARNOŠ et. al.

Základní údaje

Originální název

Linking planar polarity signalling to actomyosin contractility during vertebrate neurulation

Autoři

NOVOTNÁ, Šárka (203 Česká republika, domácí); Lorena AGOSTINI MAIA (76 Brazílie, domácí); Katarzyna Anna RADASZKIEWICZ (616 Polsko, domácí); Pavel ROUDNICKÝ (203 Česká republika, domácí) a Jakub HARNOŠ (203 Česká republika, domácí)

Vydání

Open Biology, Royal Society, 2024, 2046-2441

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10605 Developmental biology

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.500 v roce 2023

Kód RIV

RIV/00216224:14310/24:00137747

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1098/rsob.240251

UT WoS

001357928300002

EID Scopus

2-s2.0-85209909295

Klíčová slova anglicky

actomyosin contractility; planar cell polarity; vertebrates; neurulation; Xenopus embryos; MDCK cells

Štítky

CF CELLIM, CF PROT, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 24. 3. 2025 00:23, Mgr. Eva Dubská

Anotace

V originále

Actomyosin contractility represents an ancient feature of eukaryotic cells participating in many developmental and homeostasis events, including tissue morphogenesis, muscle contraction and cell migration, with dysregulation implicated in various pathological conditions, such as cancer. At the molecular level, actomyosin comprises actin bundles and myosin motor proteins that are sensitive to posttranslational modifications like phosphorylation. While the molecular components of actomyosin are well understood, the coordination of contractility by extracellular and intracellular signals, particularly from cellular signalling pathways, remains incompletely elucidated. This study focuses on WNT/planar cell polarity (PCP) signalling, previously associated with actomyosin contractility during vertebrate neurulation. Our investigation reveals that the main cytoplasmic PCP proteins, Prickle and Dishevelled, interact with key actomyosin components such as myosin light chain 9 (MLC9), leading to its phosphorylation and localized activation. Using proteomics and microscopy approaches, we demonstrate that both PCP proteins actively control actomyosin contractility through Rap1 small GTPases in relevant in vitro and in vivo models. These findings unveil a novel mechanism of how PCP signalling regulates actomyosin contractility through MLC9 and Rap1 that is relevant to vertebrate neurulation.

Návaznosti

EF18_046/0015974, projekt VaV

Název: Modernizace České infrastruktury pro integrativní strukturní biologii

GA22-06405S, projekt VaV

Název: Odhalení mechanismu formování nervové trubice

Investor: Grantová agentura ČR, Odhalení mechanismu formování nervové trubice

MUNI/J/0004/2021, interní kód MU

Název: Role of the Planar Cell Polarity and Mitochondria Crosstalk in Fuelling Cell Migration

Investor: Masarykova univerzita, Role of the Planar Cell Polarity and Mitochondria Crosstalk in Fuelling Cell Migration, MASH JUNIOR - MUNI Award In Science and Humanities JUNIOR

90242, velká výzkumná infrastruktura

Název: CIISB III

90250, velká výzkumná infrastruktura

Název: Czech-BioImaging III

Citovat

NOVOTNÁ, Šárka; Lorena AGOSTINI MAIA; Katarzyna Anna RADASZKIEWICZ; Pavel ROUDNICKÝ a Jakub HARNOŠ. Linking planar polarity signalling to actomyosin contractility during vertebrate neurulation. Open Biology. Royal Society, 2024, roč. 14, č. 11, s. 240251-240265. ISSN 2046-2441. Dostupné z: https://dx.doi.org/10.1098/rsob.240251.

@article{2454739,
   author = {Novotná, Šárka and Agostini Maia, Lorena and Radaszkiewicz, Katarzyna Anna and Roudnický, Pavel and Harnoš, Jakub},
   article_number = {11},
   doi = {http://dx.doi.org/10.1098/rsob.240251},
   keywords = {actomyosin contractility; planar cell polarity; vertebrates; neurulation; Xenopus embryos; MDCK cells},
   language = {eng},
   issn = {2046-2441},
   journal = {Open Biology},
   title = {Linking planar polarity signalling to actomyosin contractility during vertebrate neurulation},
   url = {https://royalsocietypublishing.org/doi/10.1098/rsob.240251},
   volume = {14},
   year = {2024}
}

TY  - JOUR
ID  - 2454739
AU  - Novotná, Šárka - Agostini Maia, Lorena - Radaszkiewicz, Katarzyna Anna - Roudnický, Pavel - Harnoš, Jakub
PY  - 2024
TI  - Linking planar polarity signalling to actomyosin contractility during vertebrate neurulation
JF  - Open Biology
VL  - 14
IS  - 11
SP  - 240251
EP  - 240251
PB  - Royal Society
SN  - 20462441
KW  - actomyosin contractility
KW  - planar cell polarity
KW  - vertebrates
KW  - neurulation
KW  - Xenopus embryos
KW  - MDCK cells
UR  - https://royalsocietypublishing.org/doi/10.1098/rsob.240251
N2  - Actomyosin contractility represents an ancient feature of eukaryotic cells participating in many developmental and homeostasis events, including tissue morphogenesis, muscle contraction and cell migration, with dysregulation implicated in various pathological conditions, such as cancer. At the molecular level, actomyosin comprises actin bundles and myosin motor proteins that are sensitive to posttranslational modifications like phosphorylation. While the molecular components of actomyosin are well understood, the coordination of contractility by extracellular and intracellular signals, particularly from cellular signalling pathways, remains incompletely elucidated. This study focuses on WNT/planar cell polarity (PCP) signalling, previously associated with actomyosin contractility during vertebrate neurulation. Our investigation reveals that the main cytoplasmic PCP proteins, Prickle and Dishevelled, interact with key actomyosin components such as myosin light chain 9 (MLC9), leading to its phosphorylation and localized activation. Using proteomics and microscopy approaches, we demonstrate that both PCP proteins actively control actomyosin contractility through Rap1 small GTPases in relevant in vitro and in vivo models. These findings unveil a novel mechanism of how PCP signalling regulates actomyosin contractility through MLC9 and Rap1 that is relevant to vertebrate neurulation.
ER  -

NOVOTNÁ, Šárka; Lorena AGOSTINI MAIA; Katarzyna Anna RADASZKIEWICZ; Pavel ROUDNICKÝ a Jakub HARNOŠ. Linking planar polarity signalling to actomyosin contractility during vertebrate neurulation. \textit{Open Biology}. Royal Society, 2024, roč.~14, č.~11, s.~240251-240265. ISSN~2046-2441. Dostupné z: https://dx.doi.org/10.1098/rsob.240251.

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