Spontaneous electrical activity of patient-specific
cardiomyocytes with a variant in the RYR2 gene recorded by
multielectrode array technique

k 2024

Spontaneous electrical activity of patient-specific cardiomyocytes with a variant in the RYR2 gene recorded by multielectrode array technique

KRÁL, Martin, Olga ŠVECOVÁ, Štefan ZELENÁK, Jiří PACHERNÍK, Tomáš BÁRTA et. al.

Základní údaje

Originální název

Spontaneous electrical activity of patient-specific cardiomyocytes with a variant in the RYR2 gene recorded by multielectrode array technique

Autoři

KRÁL, Martin (203 Česká republika, domácí), Olga ŠVECOVÁ (203 Česká republika, domácí), Štefan ZELENÁK (703 Slovensko), Jiří PACHERNÍK (203 Česká republika), Tomáš BÁRTA (203 Česká republika), Jana ZÍDKOVÁ (203 Česká republika), Iva SYNKOVÁ (203 Česká republika), Samuel LIETAVA (703 Slovensko), Tomáš NOVOTNÝ (203 Česká republika) a Markéta BÉBAROVÁ (203 Česká republika, garant, domácí)

Vydání

International Union of Physiological Science 2024 Beacon Meeting in Tutzing, 2024

Další údaje

Jazyk

angličtina

Typ výsledku

Prezentace na konferencích

Obor

30105 Physiology

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

hiPSC-CM; RYR2; multielectrode array; MEA

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 19. 12. 2024 07:16, Mgr. Martin Král

Anotace

V originále

Background: Y4734C variant in the ryanodine receptor type 2 (RYR2) was found in a patient with idiopathic ventricular fibrillation and his sister who was diagnosed with catecholaminergic polymorphic ventricular tachycardia (CPVT). The variant hasn´t been functionally tested before. To reveal the proarrhythmic potential of the variant, cardiomyocytes derived from human-induced pluripotent stem cells (hiPSC-CM) of the proband (IF), his sister (CPVT), and his healthy nephew as control (WT) were prepared. Here we bring data showing the spontaneous electrical activity of the hiPSC-CM and its changes under β-adrenergic stimulation. Methods: The spontaneous electrical activity of hiPSC-CM in control conditions (CC) and under the effect of 0.5μM isoprenaline (ISO) stimulating β-adrenergic receptors was recorded by the multielectrode array technique at 37°C. The cycle length (CL) and field potential duration (FPD) were evaluated; Bazett´s formula was used to correct FPD (FPDc). The short-term variability of CL (STVCL) was calculated using Origin2024 software. The data are presented as the median±interquartile range of n samples (nWT=11; nIF=19; nCPVT=18), and non-parametric tests (Wilcoxon and Kruskal-Wallis) were used due to non-normal data distribution; P˂0.05 was considered statistically significant. Results: CL was significantly shorter in WT (0.71±0.29s) than in CPVT (1.40±0.81s); IF did not differ from both (1.10±1.23s). There was no difference between WT, IF, and CPVT in FPD (152.1±106.3, 148.5±75.9, and 128.0±46.0ms, respectively), however, FPDc was significantly longer in WT (179.1±139.1ms) than in CPVT (94.4±41.7ms). After adding ISO, CL and FPD were significantly shortened in all groups (WT CLISO=0.62±0.20s, FPDISO=135.2±58.3ms; IF CLISO=0.67±0.84s, FPDISO=118.7±71.8ms; CPVT CLISO=1.12±0.80s, FPDISO=104.4±25.7ms); no significant changes were observed in FPDc. The CPVT showed significantly larger shortening in CL than WT and IF (by 38.7±21.6, 13.8±23.3, and 25.7±28.5%, respectively) whereas no differences were apparent in FPD shortening. IF and CPVT showed significantly larger STVCL than WT in CC (18.3±45.1, 34.0±42.6, and 3.9±13.2ms, respectively). ISO significantly decreased STVCL in both IF and CPVT (9.0±27.8 and 8.5±7.8ms, respectively) but had no significant effect on WT (8.1±42.7ms). Conclusions: CPVT showed a slower beating rate than WT and higher reactivity to ISO than WT and IF. ISO increased beating frequency and shortened FPD in every group. IF and CPVT had higher beat-to-beat variability of CL than WT in CC, but no difference among groups was apparent under ISO.

Návaznosti

MUNI/A/1547/2023, interní kód MU

Název: Analýza (dys)funkce: od molekul k živému organismu

Investor: Masarykova univerzita, Analýza (dys)funkce: od molekul k živému organismu

NU22-02-00348, projekt VaV

Název: Funkční hodnocení genetických variant u případů klinicky „skutečné“ idiopatické fibrilace komor: in vitro a in silico modelování s cílem odhalit arytmogenní mechanismus

Investor: Ministerstvo zdravotnictví ČR, Funkční hodnocení genetických variant u případů klinicky „skutečné“ idiopatické fibrilace komor: in vitro a in silico modelování s cílem odhalit arytmogenní mechanismus, Podprogram 1 - standardní

Citovat

KRÁL, Martin, Olga ŠVECOVÁ, Štefan ZELENÁK, Jiří PACHERNÍK, Tomáš BÁRTA, Jana ZÍDKOVÁ, Iva SYNKOVÁ, Samuel LIETAVA, Tomáš NOVOTNÝ a Markéta BÉBAROVÁ. Spontaneous electrical activity of patient-specific cardiomyocytes with a variant in the RYR2 gene recorded by multielectrode array technique. In International Union of Physiological Science 2024 Beacon Meeting in Tutzing. 2024.

@proceedings{2461498,
   author = {Král, Martin and Švecová, Olga and Zelenák, Štefan and Pacherník, Jiří and Bárta, Tomáš and Zídková, Jana and Synková, Iva and Lietava, Samuel and Novotný, Tomáš and Bébarová, Markéta},
   booktitle = {International Union of Physiological Science 2024 Beacon Meeting in Tutzing},
   keywords = {hiPSC-CM; RYR2; multielectrode array; MEA},
   language = {eng},
   title = {Spontaneous electrical activity of patient-specific cardiomyocytes with a variant in the RYR2 gene recorded by multielectrode array technique},
   year = {2024}
}

TY  - CONF
ID  - 2461498
AU  - Král, Martin - Švecová, Olga - Zelenák, Štefan - Pacherník, Jiří - Bárta, Tomáš - Zídková, Jana - Synková, Iva - Lietava, Samuel - Novotný, Tomáš - Bébarová, Markéta
PY  - 2024
TI  - Spontaneous electrical activity of patient-specific cardiomyocytes with a variant in the RYR2 gene recorded by multielectrode array technique
KW  - hiPSC-CM
KW  - RYR2
KW  - multielectrode array
KW  - MEA
N2  - Background: Y4734C variant in the ryanodine receptor type 2 (RYR2) was found in a patient with idiopathic ventricular fibrillation and his sister who was diagnosed with catecholaminergic polymorphic ventricular tachycardia (CPVT). The variant hasn´t been functionally tested before. To reveal the proarrhythmic potential of the variant, cardiomyocytes derived from human-induced pluripotent stem cells (hiPSC-CM) of the proband (IF), his sister (CPVT), and his healthy nephew as control (WT) were prepared. Here we bring data showing the spontaneous electrical activity of the hiPSC-CM and its changes under β-adrenergic stimulation. Methods: The spontaneous electrical activity of hiPSC-CM in control conditions (CC) and under the effect of 0.5μM isoprenaline (ISO) stimulating β-adrenergic receptors was recorded by the multielectrode array technique at 37°C. The cycle length (CL) and field potential duration (FPD) were evaluated; Bazett´s formula was used to correct FPD (FPDc). The short-term variability of CL (STVCL) was calculated using Origin2024 software. The data are presented as the median±interquartile range of n samples (nWT=11; nIF=19; nCPVT=18), and non-parametric tests (Wilcoxon and Kruskal-Wallis) were used due to non-normal data distribution; P˂0.05 was considered statistically significant. Results: CL was significantly shorter in WT (0.71±0.29s) than in CPVT (1.40±0.81s); IF did not differ from both (1.10±1.23s). There was no difference between WT, IF, and CPVT in FPD (152.1±106.3, 148.5±75.9, and 128.0±46.0ms, respectively), however, FPDc was significantly longer in WT (179.1±139.1ms) than in CPVT (94.4±41.7ms). After adding ISO, CL and FPD were significantly shortened in all groups (WT CLISO=0.62±0.20s, FPDISO=135.2±58.3ms; IF CLISO=0.67±0.84s, FPDISO=118.7±71.8ms; CPVT CLISO=1.12±0.80s, FPDISO=104.4±25.7ms); no significant changes were observed in FPDc. The CPVT showed significantly larger shortening in CL than WT and IF (by 38.7±21.6, 13.8±23.3, and 25.7±28.5%, respectively) whereas no differences were apparent in FPD shortening. IF and CPVT showed significantly larger STVCL than WT in CC (18.3±45.1, 34.0±42.6, and 3.9±13.2ms, respectively). ISO significantly decreased STVCL in both IF and CPVT (9.0±27.8 and 8.5±7.8ms, respectively) but had no significant effect on WT (8.1±42.7ms). Conclusions: CPVT showed a slower beating rate than WT and higher reactivity to ISO than WT and IF. ISO increased beating frequency and shortened FPD in every group. IF and CPVT had higher beat-to-beat variability of CL than WT in CC, but no difference among groups was apparent under ISO.
ER  -

KRÁL, Martin, Olga ŠVECOVÁ, Štefan ZELENÁK, Jiří PACHERNÍK, Tomáš BÁRTA, Jana ZÍDKOVÁ, Iva SYNKOVÁ, Samuel LIETAVA, Tomáš NOVOTNÝ a Markéta BÉBAROVÁ. Spontaneous electrical activity of patient-specific cardiomyocytes with a variant in the RYR2 gene recorded by multielectrode array technique. In \textit{International Union of Physiological Science 2024 Beacon Meeting in Tutzing}. 2024.

Podrobný výpis o publikaci