Growth Phase Matters: Boosting immunity via
Lacticasebacillus-derived membrane vesicles and their
interactions with TLR2 pathways

J 2024

Growth Phase Matters: Boosting immunity via Lacticasebacillus-derived membrane vesicles and their interactions with TLR2 pathways

SANDANUSOVÁ, Miriam; Kristyna TURKOVA; Eva PECHÁČKOVÁ; Jan KOTOUCEK; Pavel ROUDNICKÝ et. al.

Základní údaje

Originální název

Growth Phase Matters: Boosting immunity via Lacticasebacillus-derived membrane vesicles and their interactions with TLR2 pathways

Autoři

SANDANUSOVÁ, Miriam (703 Slovensko, domácí); Kristyna TURKOVA (203 Česká republika); Eva PECHÁČKOVÁ (203 Česká republika, domácí); Jan KOTOUCEK (203 Česká republika); Pavel ROUDNICKÝ (203 Česká republika, domácí); Martin ŠINDELÁŘ (203 Česká republika, domácí); Lukáš KUBALA (203 Česká republika, domácí) a Gabriela AMBROZOVA (203 Česká republika)

Vydání

Journal of Extracellular Biology, Wiley, 2024, 2768-2811

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Kód RIV

RIV/00216224:14310/24:00138454

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1002/jex2.169

UT WoS

001533318700001

EID Scopus

2-s2.0-85208419294

Klíčová slova anglicky

growth curve; immunomodulation; Lacticaseibacillus rhamnosus; lipoteichoic acid; membrane vesicles; nanocarriers; TLR2

Štítky

CF BIC, CF CRYO, CF PROT, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 28. 7. 2025 10:42, Mgr. Michal Petr

Anotace

V originále

Lipid bi-layered particles known as membrane vesicles (MVs), produced by Gram-positive bacteria are a communication tool throughout the entire bacterial growth. However, the MVs characteristics may vary across all stages of maternal culture growth, leading to inconsistencies in MVs research. This, in turn, hinders their employment as nanocarriers, vaccines and other medical applications. In this study, we aimed to comprehensively characterize MVs derived from Lacticaseibacillus rhamnosus CCM7091 isolated at different growth stages: early exponential (6 h, MV6), late exponential (12 h, MV12) and late stationary phase (48 h, MV48). We observed significant differences in protein content between MV6 and MV48 (data are available via ProteomeXchange with identifier PXD041580), likely contributing to their different immunomodulatory capacities. In vitro analysis demonstrated that MV48 uptake rate by epithelial Caco-2 cells is significantly higher and they stimulate an immune response in murine macrophages RAW 264.7 (elevated production of TNFα, IL-6, IL-10, NO). This correlated with increased expression of lipoteichoic acid (LTA) and enhanced TLR2 signalling in MV48, suggesting that LTA contributes to the immunomodulation. In conclusion, we showed that Lacticaseibacillus rhamnosus CCM7091-derived MVs from the late stationary phase boost the immune response the most effectively, which pre-destines them for therapeutical application as nanocarriers.

Návaznosti

EF18_046/0015974, projekt VaV

Název: Modernizace České infrastruktury pro integrativní strukturní biologii

LX22NPO5104, projekt VaV

Název: Národní institut pro výzkum metabolických a kardiovaskulárních onemocnění (Akronym: CarDia)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Národní institut pro léčbu metabolických a kardiovaskulárních onemocnění, 5.1 EXCELES

MUNI/C/0023/2020, interní kód MU

Název: Intercellular communication in the development of peritoneal adhesions – focused on the role of extracellular vesicles

Investor: Masarykova univerzita, Intercellular communication in the development of peritoneal adhesions – focused on the role of extracellular vesicles, DO R. 2020 - Program rektora

90242, velká výzkumná infrastruktura

Název: CIISB III

Přiložené soubory

Growth_Phase_Matters_Boosting_immunity_via_Lacticasebacillus-derived_membrane_vesicles_and_their_interactions_with_TLR2_pathways.pdf

Citovat

SANDANUSOVÁ, Miriam; Kristyna TURKOVA; Eva PECHÁČKOVÁ; Jan KOTOUCEK; Pavel ROUDNICKÝ; Martin ŠINDELÁŘ; Lukáš KUBALA a Gabriela AMBROZOVA. Growth Phase Matters: Boosting immunity via Lacticasebacillus-derived membrane vesicles and their interactions with TLR2 pathways. Journal of Extracellular Biology. Wiley, 2024, roč. 3, č. 8, s. 1-19. ISSN 2768-2811. Dostupné z: https://dx.doi.org/10.1002/jex2.169.

@article{2467857,
   author = {Sandanusová, Miriam and Turkova, Kristyna and Pecháčková, Eva and Kotoucek, Jan and Roudnický, Pavel and Šindelář, Martin and Kubala, Lukáš and Ambrozova, Gabriela},
   article_number = {8},
   doi = {http://dx.doi.org/10.1002/jex2.169},
   keywords = {growth curve; immunomodulation; Lacticaseibacillus rhamnosus; lipoteichoic acid; membrane vesicles; nanocarriers; TLR2},
   language = {eng},
   issn = {2768-2811},
   journal = {Journal of Extracellular Biology},
   title = {Growth Phase Matters: Boosting immunity via Lacticasebacillus-derived membrane vesicles and their interactions with TLR2 pathways},
   url = {https://isevjournals.onlinelibrary.wiley.com/doi/10.1002/jex2.169},
   volume = {3},
   year = {2024}
}

TY  - JOUR
ID  - 2467857
AU  - Sandanusová, Miriam - Turkova, Kristyna - Pecháčková, Eva - Kotoucek, Jan - Roudnický, Pavel - Šindelář, Martin - Kubala, Lukáš - Ambrozova, Gabriela
PY  - 2024
TI  - Growth Phase Matters: Boosting immunity via Lacticasebacillus-derived membrane vesicles and their interactions with TLR2 pathways
JF  - Journal of Extracellular Biology
VL  - 3
IS  - 8
SP  - 1-19
EP  - 1-19
PB  - Wiley
SN  - 27682811
KW  - growth curve
KW  - immunomodulation
KW  - Lacticaseibacillus rhamnosus
KW  - lipoteichoic acid
KW  - membrane vesicles
KW  - nanocarriers
KW  - TLR2
UR  - https://isevjournals.onlinelibrary.wiley.com/doi/10.1002/jex2.169
N2  - Lipid bi-layered particles known as membrane vesicles (MVs), produced by Gram-positive bacteria are a communication tool throughout the entire bacterial growth. However, the MVs characteristics may vary across all stages of maternal culture growth, leading to inconsistencies in MVs research. This, in turn, hinders their employment as nanocarriers, vaccines and other medical applications. In this study, we aimed to comprehensively characterize MVs derived from Lacticaseibacillus rhamnosus CCM7091 isolated at different growth stages: early exponential (6 h, MV6), late exponential (12 h, MV12) and late stationary phase (48 h, MV48). We observed significant differences in protein content between MV6 and MV48 (data are available via ProteomeXchange with identifier PXD041580), likely contributing to their different immunomodulatory capacities. In vitro analysis demonstrated that MV48 uptake rate by epithelial Caco-2 cells is significantly higher and they stimulate an immune response in murine macrophages RAW 264.7 (elevated production of TNFα, IL-6, IL-10, NO). This correlated with increased expression of lipoteichoic acid (LTA) and enhanced TLR2 signalling in MV48, suggesting that LTA contributes to the immunomodulation. In conclusion, we showed that Lacticaseibacillus rhamnosus CCM7091-derived MVs from the late stationary phase boost the immune response the most effectively, which pre-destines them for therapeutical application as nanocarriers.
ER  -

SANDANUSOVÁ, Miriam; Kristyna TURKOVA; Eva PECHÁČKOVÁ; Jan KOTOUCEK; Pavel ROUDNICKÝ; Martin ŠINDELÁŘ; Lukáš KUBALA a Gabriela AMBROZOVA. Growth Phase Matters: Boosting immunity via Lacticasebacillus-derived membrane vesicles and their interactions with TLR2 pathways. \textit{Journal of Extracellular Biology}. Wiley, 2024, roč.~3, č.~8, s.~1-19. ISSN~2768-2811. Dostupné z: https://dx.doi.org/10.1002/jex2.169.

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