Penton blooming, a conserved mechanism of genome delivery used
by disparate microviruses

J 2025

Penton blooming, a conserved mechanism of genome delivery used by disparate microviruses

BARDY, Pavol; Conor I. W. MACDONALD; Paul C. KIRCHBERGER; Huw T. JENKINS; Tibor BOTKA et al.

Základní údaje

Originální název

Penton blooming, a conserved mechanism of genome delivery used by disparate microviruses

Autoři

Vydání

mBio, American Society for Microbiology, 2025, 2150-7511

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10606 Microbiology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.700 v roce 2024

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/25:00140858

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

electron microscopy; structural biology; Microviridae; virion structure; Rhodobacter

Štítky

14314010, NIVB_PřF, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 24. 9. 2025 18:14, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

Microviruses are single-stranded DNA viruses infecting bacteria, characterized by T = 1 shells made of single jelly-roll capsid proteins. To understand how microviruses infect their host cells, we have isolated and studied an unusually large microvirus, Ebor. Ebor belongs to the proposed “Tainavirinae” subfamily of Microviridae and infects the model Alphaproteobacterium Rhodobacter capsulatus. Using cryogenic electron microscopy, we show that the enlarged capsid of Ebor is the result of an extended C-terminus of the major capsid protein. The extra packaging space accommodates genes encoding a lytic enzyme and putative methylase, both absent in microviruses with shorter genomes. The capsid is decorated with protrusions at its 3-fold axes, which we show to recognize lipopolysaccharides on the host surface. Cryogenic electron tomography shows that during infection, Ebor attaches to the host cell via five such protrusions. This attachment brings a single pentameric capsomer into close contact with the cell membrane, creating a special vertex through which the genome is ejected. Both subtomogram averaging and single particle analysis identified two intermediates of capsid opening, showing that the interacting penton opens from its center via the separation of individual capsomer subunits. Structural comparison with the model Bullavirinae phage phiX174 suggests that this genome delivery mechanism may be widely present across Microviridae.

Návaznosti

LX22NPO5103, projekt VaV

Název: Národní institut virologie a bakteriologie (Akronym: NIVB)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Národní institut virologie a bakteriologie, 5.1 EXCELES

Přiložené soubory

2485318.pdf

2485318_published.pdf

Citovat

BARDY, Pavol; Conor I. W. MACDONALD; Paul C. KIRCHBERGER; Huw T. JENKINS; Tibor BOTKA; Lewis BYROM; Nawshin T. B. ALIM; Daouda A. K. TRAORE; Hannah C. KOENIG; Tristan R. NICHOLAS; Maria CHECHIK; Samuel J. HART; Johan P. TURKENBURG; James N. BLAZA; J. Thomas BEATTY; Paul C. M. FOGG a Alfred A. ANTSON. Penton blooming, a conserved mechanism of genome delivery used by disparate microviruses. mBio. American Society for Microbiology, 2025, roč. 16, č. 4, s. 1-23. ISSN 2150-7511. Dostupné z: https://doi.org/10.1128/mbio.03713-24.

@article{2485318,
   author = {Bardy, Pavol and MacDonald, Conor I. W. and Kirchberger, Paul C. and Jenkins, Huw T. and Botka, Tibor and Byrom, Lewis and Alim, Nawshin T. B. and Traore, Daouda A. K. and Koenig, Hannah C. and Nicholas, Tristan R. and Chechik, Maria and Hart, Samuel J. and Turkenburg, Johan P. and Blaza, James N. and Beatty, J. Thomas and Fogg, Paul C. M. and Antson, Alfred A.},
   article_number = {4},
   doi = {https://doi.org/10.1128/mbio.03713-24},
   keywords = {electron microscopy; structural biology; Microviridae; virion structure; Rhodobacter},
   language = {eng},
   issn = {2150-7511},
   journal = {mBio},
   title = {Penton blooming, a conserved mechanism of genome delivery used by disparate microviruses},
   url = {https://doi.org/10.1128/mbio.03713-24},
   volume = {16},
   year = {2025}
}

TY  - JOUR
ID  - 2485318
AU  - Bardy, Pavol - MacDonald, Conor I. W. - Kirchberger, Paul C. - Jenkins, Huw T. - Botka, Tibor - Byrom, Lewis - Alim, Nawshin T. B. - Traore, Daouda A. K. - Koenig, Hannah C. - Nicholas, Tristan R. - Chechik, Maria - Hart, Samuel J. - Turkenburg, Johan P. - Blaza, James N. - Beatty, J. Thomas - Fogg, Paul C. M. - Antson, Alfred A.
PY  - 2025
TI  - Penton blooming, a conserved mechanism of genome delivery used by disparate microviruses
JF  - mBio
VL  - 16
IS  - 4
SP  - 1-23
EP  - 1-23
PB  - American Society for Microbiology
SN  - 21507511
KW  - electron microscopy
KW  - structural biology
KW  - Microviridae
KW  - virion structure
KW  - Rhodobacter
UR  - https://doi.org/10.1128/mbio.03713-24
N2  - Microviruses are single-stranded DNA viruses infecting bacteria, characterized by T = 1 shells made of single jelly-roll capsid proteins. To understand how microviruses infect their host cells, we have isolated and studied an unusually large microvirus, Ebor. Ebor belongs to the proposed “Tainavirinae” subfamily of Microviridae and infects the model Alphaproteobacterium Rhodobacter capsulatus. Using cryogenic electron microscopy, we show that the enlarged capsid of Ebor is the result of an extended C-terminus of the major capsid protein. The extra packaging space accommodates genes encoding a lytic enzyme and putative methylase, both absent in microviruses with shorter genomes. The capsid is decorated with protrusions at its 3-fold axes, which we show to recognize lipopolysaccharides on the host surface. Cryogenic electron tomography shows that during infection, Ebor attaches to the host cell via five such protrusions. This attachment brings a single pentameric capsomer into close contact with the cell membrane, creating a special vertex through which the genome is ejected. Both subtomogram averaging and single particle analysis identified two intermediates of capsid opening, showing that the interacting penton opens from its center via the separation of individual capsomer subunits. Structural comparison with the model Bullavirinae phage phiX174 suggests that this genome delivery mechanism may be widely present across Microviridae.
ER  -

BARDY, Pavol; Conor I. W. MACDONALD; Paul C. KIRCHBERGER; Huw T. JENKINS; Tibor BOTKA; Lewis BYROM; Nawshin T. B. ALIM; Daouda A. K. TRAORE; Hannah C. KOENIG; Tristan R. NICHOLAS; Maria CHECHIK; Samuel J. HART; Johan P. TURKENBURG; James N. BLAZA; J. Thomas BEATTY; Paul C. M. FOGG a Alfred A. ANTSON. Penton blooming, a conserved mechanism of genome delivery used by disparate microviruses. \textit{mBio}. American Society for Microbiology, 2025, roč.~16, č.~4, s.~1-23. ISSN~2150-7511. Dostupné z: https://doi.org/10.1128/mbio.03713-24.

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