PhiKZ baseplate structure

a 2025

PhiKZ baseplate structure

SEOANE BLANCO, Mateo; Miroslav HOMOLA; Tibor FÜZIK a Pavel PLEVKA

Základní údaje

Originální název

PhiKZ baseplate structure

Autoři

SEOANE BLANCO, Mateo; Miroslav HOMOLA; Tibor FÜZIK a Pavel PLEVKA

Vydání

XXI Discussions in Structural Molecular Biology and 8th User Meeting of CIISB (Czech Infrastructure for Integrative Structural Biology), Nové Hrady, 2025

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10607 Virology

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14740/25:00141390

Organizační jednotka

Středoevropský technologický institut

Klíčová slova anglicky

PhiKZ bacteriophage; CRYO-EM; genome injection systems

Štítky

NIVB_CEITEC, rivok

Příznaky

Recenzováno

Změněno: 13. 6. 2025 12:56, Mgr. Eva Dubská

Anotace

V originále

PhiKZ is a bacteriophage that infects Pseudomonas aeruginosa, an opportunistic human pathogen. The phage phiKZ is known for its large genome and complex structure, making it a notable subject for structural biology studies. Among all its parts, the baseplate is the most complex. It adheres to the phage prey and triggers the genome ejection. The signal that triggers the ejection travels from the tail fibres to the tail through the baseplate. Here, we used cryo-Electron Microscopy to visualise the structure of the phiKZ tail and the baseplate at high resolution, the first one of a jumbo bacteriophage. Our results reveal an intricate network of proteins organised in six-fold symmetry. Structural comparisons with related systems highlight the universal conservation observed in contractile injection systems. This sheds light on phiKZ’s baseplate specificities and suggests a potential mode of action. Its structural analysis enhances our understanding of phiKZ and contributes with valuable knowledge to the broader field of myovirus biology.

Návaznosti

LX22NPO5103, projekt VaV

Název: Národní institut virologie a bakteriologie (Akronym: NIVB)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Národní institut virologie a bakteriologie, 5.1 EXCELES

Přiložené soubory

SeoaneBlancoM_NoveHrady_abstract_2025.pdf

SeoaneBlancoM_NoveHrady_poster_2025.pdf

Citovat

SEOANE BLANCO, Mateo; Miroslav HOMOLA; Tibor FÜZIK a Pavel PLEVKA. PhiKZ baseplate structure. In XXI Discussions in Structural Molecular Biology and 8th User Meeting of CIISB (Czech Infrastructure for Integrative Structural Biology), Nové Hrady. 2025.

@proceedings{2503141,
   author = {Seoane Blanco, Mateo and Homola, Miroslav and Füzik, Tibor and Plevka, Pavel},
   booktitle = {XXI Discussions in Structural Molecular Biology and 8th User Meeting of CIISB (Czech Infrastructure for Integrative Structural Biology), Nové Hrady},
   keywords = {PhiKZ bacteriophage; CRYO-EM; genome injection systems},
   language = {eng},
   title = {PhiKZ baseplate structure},
   url = {https://cssb.structbio.org/xxi-discussions-in-structural-molecular-biology-and-8th-user-meeting-of-ciisb-czech-infrastructure-for-integrative-structural-biologypage/},
   year = {2025}
}

TY  - CONF
ID  - 2503141
AU  - Seoane Blanco, Mateo - Homola, Miroslav - Füzik, Tibor - Plevka, Pavel
PY  - 2025
TI  - PhiKZ baseplate structure
KW  - PhiKZ bacteriophage
KW  - CRYO-EM
KW  - genome injection systems
UR  - https://cssb.structbio.org/xxi-discussions-in-structural-molecular-biology-and-8th-user-meeting-of-ciisb-czech-infrastructure-for-integrative-structural-biologypage/
N2  - PhiKZ is a bacteriophage that infects Pseudomonas aeruginosa, an opportunistic human pathogen. The phage phiKZ is known for its large genome and complex structure, making it a notable subject for structural biology studies. Among all its parts, the baseplate is the most complex. It adheres to the phage prey and triggers the genome ejection. The signal that triggers the ejection travels from the tail fibres to the tail through the baseplate. Here, we used cryo-Electron Microscopy to visualise the structure of the phiKZ tail and the baseplate at high resolution, the first one of a jumbo bacteriophage. Our results reveal an intricate network of proteins organised in six-fold symmetry. Structural comparisons with related systems highlight the universal conservation observed in contractile injection systems. This sheds light on phiKZ’s baseplate specificities and suggests a potential mode of action. Its structural analysis enhances our understanding of phiKZ and contributes with valuable knowledge to the broader field of myovirus biology.
ER  -

SEOANE BLANCO, Mateo; Miroslav HOMOLA; Tibor FÜZIK a Pavel PLEVKA. PhiKZ baseplate structure. In \textit{XXI Discussions in Structural Molecular Biology and 8th User Meeting of CIISB (Czech Infrastructure for Integrative Structural Biology), Nové Hrady}. 2025.

Přehled o publikaci