Efficiency of sperm separation by using microfluidic chips
compared to the swim up method

J 2025

Efficiency of sperm separation by using microfluidic chips compared to the swim up method

JEŠETA, Michal; Lenka MEKIŇOVÁ; Jan HOŠEK; Pavel VENTRUBA; Igor CRHA et. al.

Základní údaje

Originální název

Efficiency of sperm separation by using microfluidic chips compared to the swim up method

Autoři

JEŠETA, Michal (203 Česká republika, domácí); Lenka MEKIŇOVÁ (703 Slovensko, domácí); Jan HOŠEK (203 Česká republika); Pavel VENTRUBA (203 Česká republika, domácí) a Igor CRHA (203 Česká republika, domácí)

Vydání

Medical Journal of Cell Biology, Germany, Sciendo, 2025, 2544-3577

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10601 Cell biology

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Organizační jednotka

Lékařská fakulta

DOI

https://doi.org/10.2478/acb-2025-0001

Klíčová slova anglicky

spermatozoa; microfluidic; DNA integrity; swim-up; sperm preparation

Štítky

rivok

Příznaky

Recenzováno

Změněno: 23. 6. 2025 10:37, Mgr. Tereza Miškechová

Anotace

V originále

The effectiveness of assisted reproduction methods is still not as high as was expected recently. For effective therapy, it is necessary to use only the highest quality sperm for oocyte fertilization, as the fertilization process most often takes place using the ICSI technique, i.e. direct injection of sperm into the oocyte. For these reasons, separation techniques are constantly being developed, and in a relatively short period of time the view of these procedures has changed. In recent years, microfluidic chip techniques have been increasingly applied. In this study, we compared the swim up technique with the two most used chips (ca0 and ZYMOT). We tested the efficiency of these separation methods against the swim-up method. In this study, we evaluated sperm concentration, motility, morphology, acrosome status and DNA integrity before and after separation methods. Each patient’s ejaculate was separated simultaneously by the swim up method and both chips. It turned out that the separation methods are very similar in motility, concentration and acrosome status and sperm morphology. However, in the case of DNA fragmentation, a significant difference in the reduction of the proportion of fragmented spermatozoa was found only with the chips and not with the swim-up method. Moreover, when comparing the relative effectiveness of sperm reduction with fragmented DNA, the ZYMOT method was more effective than Ca0. However, the differences between the chips were not statistically significant with an average of 18%.

Citovat

JEŠETA, Michal; Lenka MEKIŇOVÁ; Jan HOŠEK; Pavel VENTRUBA a Igor CRHA. Efficiency of sperm separation by using microfluidic chips compared to the swim up method. Medical Journal of Cell Biology. Germany: Sciendo, 2025, roč. 13, č. 1, s. 1-8. ISSN 2544-3577. Dostupné z: https://doi.org/10.2478/acb-2025-0001.

@article{2505104,
   author = {Ješeta, Michal and Mekiňová, Lenka and Hošek, Jan and Ventruba, Pavel and Crha, Igor},
   article_location = {Germany},
   article_number = {1},
   doi = {https://doi.org/10.2478/acb-2025-0001},
   keywords = {spermatozoa; microfluidic; DNA integrity; swim-up; sperm preparation},
   language = {eng},
   issn = {2544-3577},
   journal = {Medical Journal of Cell Biology},
   title = {Efficiency of sperm separation by using microfluidic chips compared to the swim up method},
   url = {https://sciendo.com/article/10.2478/acb-2025-0001},
   volume = {13},
   year = {2025}
}

TY  - JOUR
ID  - 2505104
AU  - Ješeta, Michal - Mekiňová, Lenka - Hošek, Jan - Ventruba, Pavel - Crha, Igor
PY  - 2025
TI  - Efficiency of sperm separation by using microfluidic chips compared to the swim up method
JF  - Medical Journal of Cell Biology
VL  - 13
IS  - 1
SP  - 1-8
EP  - 1-8
PB  - Sciendo
SN  - 25443577
KW  - spermatozoa
KW  - microfluidic
KW  - DNA integrity
KW  - swim-up
KW  - sperm preparation
UR  - https://sciendo.com/article/10.2478/acb-2025-0001
N2  - The effectiveness of assisted reproduction methods is still not as high as was expected recently. For effective therapy, it is necessary to use only the highest quality sperm for oocyte fertilization, as the fertilization process most often takes place using the ICSI technique, i.e. direct injection of sperm into the oocyte. For these reasons, separation techniques are constantly being developed, and in a relatively short period of time the view of these procedures has changed. In recent years, microfluidic chip techniques have been increasingly applied. In this study, we compared the swim up technique with the two most used chips (ca0 and ZYMOT). We tested the efficiency of these separation methods against the swim-up method. In this study, we evaluated sperm concentration, motility, morphology, acrosome status and DNA integrity before and after separation methods. Each patient’s ejaculate was separated simultaneously by the swim up method and both chips. It turned out that the separation methods are very similar in motility, concentration and acrosome status and sperm morphology. However, in the case of DNA fragmentation, a significant difference in the reduction of the proportion of fragmented spermatozoa was found only with the chips and not with the swim-up method. Moreover, when comparing the relative effectiveness of sperm reduction with fragmented DNA, the ZYMOT method was more effective than Ca0. However, the differences between the chips were not statistically significant with an average of 18%.
ER  -

JEŠETA, Michal; Lenka MEKIŇOVÁ; Jan HOŠEK; Pavel VENTRUBA a Igor CRHA. Efficiency of sperm separation by using microfluidic chips compared to the swim up method. \textit{Medical Journal of Cell Biology}. Germany: Sciendo, 2025, roč.~13, č.~1, s.~1-8. ISSN~2544-3577. Dostupné z: https://doi.org/10.2478/acb-2025-0001.

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