Pooled analysis of 3,741 stool metagenomes from 18 cohorts for
cross-stage and strain-level reproducible microbial biomarkers
of colorectal cancer

J 2025

Pooled analysis of 3,741 stool metagenomes from 18 cohorts for cross-stage and strain-level reproducible microbial biomarkers of colorectal cancer

PICCINNO, Gianmarco; Kelsey N. THOMPSON; Paolo MANGHI; Andrew R. GHAZI; Andrew Maltez THOMAS et al.

Základní údaje

Originální název

Pooled analysis of 3,741 stool metagenomes from 18 cohorts for cross-stage and strain-level reproducible microbial biomarkers of colorectal cancer

Autoři

Vydání

Nature Medicine, Nature Research, 2025, 1078-8956

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 50.000 v roce 2024

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/25:00141558

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

GUT MICROBIOTA; ASSOCIATION; RISK

Štítky

143180, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 2. 4. 2026 11:10, Mgr. Petra Trembecká, Ph.D.

Anotace

V originále

Associations between the gut microbiome and colorectal cancer (CRC) have been uncovered, but larger and more diverse studies are needed to assess their potential clinical use. We expanded upon 12 metagenomic datasets of patients with CRC (n = 930), adenomas (n = 210) and healthy control individuals (n = 976; total n = 2,116) with 6 new cohorts (n = 1,625) providing granular information on cancer stage and the anatomic location of tumors. We improved CRC prediction accuracy based solely on gut metagenomics (average area under the curve = 0.85) and highlighted the contribution of 19 newly profiled species and distinct Fusobacterium nucleatum clades. Specific gut species distinguish left-sided versus right-sided CRC (area under the curve = 0.66) with an enrichment of oral-typical microbes. We identified strain-specific CRC signatures with the commensal Ruminococcus bicirculans and Faecalibacterium prausnitzii showing subclades associated with late-stage CRC. Our analysis confirms that the microbiome can be a clinical target for CRC screening and characterizes it as a biomarker for CRC progression.

Návaznosti

EH22_008/0004644, projekt VaV

Název: Záchrana životů prostřednictvím výzkumu v oblasti včasné detekce a prevence rakoviny: Molekulární, genomické a sociální faktory

LX22NPO5102, projekt VaV

Název: Národní ústav pro výzkum rakoviny (Akronym: NÚVR)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Národní ústav pro výzkum rakoviny, 5.1 EXCELES

825410, interní kód MU

Název: Gut OncoMicrobiome Signatures (GOMS) associated with cancer incidence, prognosis and prediction of treatment response (Akronym: ONCOBIOME)

Investor: Evropská unie, Gut OncoMicrobiome Signatures (GOMS) associated with cancer incidence, prognosis and prediction of treatment response, Health, demographic change and wellbeing (Societal Challenges)

857560, interní kód MU
(Kód CEP: EF17_043/0009632)

Název: CETOCOEN Excellence (Akronym: CETOCOEN Excellence)

Investor: Evropská unie, CETOCOEN Excellence, Spreading excellence and widening participation

90233, velká výzkumná infrastruktura

Název: BBMRI.cz IV

90269, velká výzkumná infrastruktura

Název: RECETOX RI II

Přiložené soubory

2507852.pdf

2507852_published_version.pdf

Citovat

PICCINNO, Gianmarco; Kelsey N. THOMPSON; Paolo MANGHI; Andrew R. GHAZI; Andrew Maltez THOMAS; Aitor BLANCO-MIGUEZ; Francesco ASNICAR; Katarina MLADENOVIC; Federica PINTO; Federica ARMANINI; Michal PUNCOCHAR; Elisa PIPERNI; Vitor HEIDRICH; Gloria FACKELMANN; Giulio FERRERO; Sonia TARALLO; Long H. NGUYEN; Yan YAN; Nazim A. KELES; Bilge G. TUNA; Veronika VYMETALKOVA; Mario TROMPETTO; Vaclav LISKA; Tomas HUCL; Pavel VODICKA; Beatrix BENCSIKOVA; Martina ČARNOGURSKÁ; Vlad POPOVICI; Federica MARMORINO; Chiara CREMOLINI; Barbara PARDINI; Francesca CORDERO; Mingyang SONG; Andrew T. CHAN; Lisa DEROSA; Laurence ZITVOGEL; Curtis HUTTENHOWER; Alessio NACCARATI; Eva BUDINSKÁ a Nicola SEGATA. Pooled analysis of 3,741 stool metagenomes from 18 cohorts for cross-stage and strain-level reproducible microbial biomarkers of colorectal cancer. Nature Medicine. Nature Research, 2025, roč. 31, č. 7, s. 2416-2429. ISSN 1078-8956. Dostupné z: https://doi.org/10.1038/s41591-025-03693-9.

@article{2507852,
   author = {Piccinno, Gianmarco and Thompson, Kelsey N. and Manghi, Paolo and Ghazi, Andrew R. and Thomas, Andrew Maltez and BlancoandMiguez, Aitor and Asnicar, Francesco and Mladenovic, Katarina and Pinto, Federica and Armanini, Federica and Puncochar, Michal and Piperni, Elisa and Heidrich, Vitor and Fackelmann, Gloria and Ferrero, Giulio and Tarallo, Sonia and Nguyen, Long H. and Yan, Yan and Keles, Nazim A. and Tuna, Bilge G. and Vymetalkova, Veronika and Trompetto, Mario and Liska, Vaclav and Hucl, Tomas and Vodicka, Pavel and Bencsikova, Beatrix and Čarnogurská, Martina and Popovici, Vlad and Marmorino, Federica and Cremolini, Chiara and Pardini, Barbara and Cordero, Francesca and Song, Mingyang and Chan, Andrew T. and Derosa, Lisa and Zitvogel, Laurence and Huttenhower, Curtis and Naccarati, Alessio and Budinská, Eva and Segata, Nicola},
   article_number = {7},
   doi = {https://doi.org/10.1038/s41591-025-03693-9},
   keywords = {GUT MICROBIOTA; ASSOCIATION; RISK},
   language = {eng},
   issn = {1078-8956},
   journal = {Nature Medicine},
   note = {Nedoplňovat EF17_043/0009632.},
   title = {Pooled analysis of 3,741 stool metagenomes from 18 cohorts for cross-stage and strain-level reproducible microbial biomarkers of colorectal cancer},
   url = {https://www.nature.com/articles/s41591-025-03693-9},
   volume = {31},
   year = {2025}
}

TY  - JOUR
ID  - 2507852
AU  - Piccinno, Gianmarco - Thompson, Kelsey N. - Manghi, Paolo - Ghazi, Andrew R. - Thomas, Andrew Maltez - Blanco-Miguez, Aitor - Asnicar, Francesco - Mladenovic, Katarina - Pinto, Federica - Armanini, Federica - Puncochar, Michal - Piperni, Elisa - Heidrich, Vitor - Fackelmann, Gloria - Ferrero, Giulio - Tarallo, Sonia - Nguyen, Long H. - Yan, Yan - Keles, Nazim A. - Tuna, Bilge G. - Vymetalkova, Veronika - Trompetto, Mario - Liska, Vaclav - Hucl, Tomas - Vodicka, Pavel - Bencsikova, Beatrix - Čarnogurská, Martina - Popovici, Vlad - Marmorino, Federica - Cremolini, Chiara - Pardini, Barbara - Cordero, Francesca - Song, Mingyang - Chan, Andrew T. - Derosa, Lisa - Zitvogel, Laurence - Huttenhower, Curtis - Naccarati, Alessio - Budinská, Eva - Segata, Nicola
PY  - 2025
TI  - Pooled analysis of 3,741 stool metagenomes from 18 cohorts for cross-stage and strain-level reproducible microbial biomarkers of colorectal cancer
JF  - Nature Medicine
VL  - 31
IS  - 7
SP  - 2416-2429
EP  - 2416-2429
PB  - Nature Research
SN  - 10788956
N1  - Nedoplňovat EF17_043/0009632.
KW  - GUT MICROBIOTA
KW  - ASSOCIATION
KW  - RISK
UR  - https://www.nature.com/articles/s41591-025-03693-9
N2  - Associations between the gut microbiome and colorectal cancer (CRC) have been uncovered, but larger and more diverse studies are needed to assess their potential clinical use. We expanded upon 12 metagenomic datasets of patients with CRC (n = 930), adenomas (n = 210) and healthy control individuals (n = 976; total n = 2,116) with 6 new cohorts (n = 1,625) providing granular information on cancer stage and the anatomic location of tumors. We improved CRC prediction accuracy based solely on gut metagenomics (average area under the curve = 0.85) and highlighted the contribution of 19 newly profiled species and distinct Fusobacterium nucleatum clades. Specific gut species distinguish left-sided versus right-sided CRC (area under the curve = 0.66) with an enrichment of oral-typical microbes. We identified strain-specific CRC signatures with the commensal Ruminococcus bicirculans and Faecalibacterium prausnitzii showing subclades associated with late-stage CRC. Our analysis confirms that the microbiome can be a clinical target for CRC screening and characterizes it as a biomarker for CRC progression.
ER  -

PICCINNO, Gianmarco; Kelsey N. THOMPSON; Paolo MANGHI; Andrew R. GHAZI; Andrew Maltez THOMAS; Aitor BLANCO-MIGUEZ; Francesco ASNICAR; Katarina MLADENOVIC; Federica PINTO; Federica ARMANINI; Michal PUNCOCHAR; Elisa PIPERNI; Vitor HEIDRICH; Gloria FACKELMANN; Giulio FERRERO; Sonia TARALLO; Long H. NGUYEN; Yan YAN; Nazim A. KELES; Bilge G. TUNA; Veronika VYMETALKOVA; Mario TROMPETTO; Vaclav LISKA; Tomas HUCL; Pavel VODICKA; Beatrix BENCSIKOVA; Martina ČARNOGURSKÁ; Vlad POPOVICI; Federica MARMORINO; Chiara CREMOLINI; Barbara PARDINI; Francesca CORDERO; Mingyang SONG; Andrew T. CHAN; Lisa DEROSA; Laurence ZITVOGEL; Curtis HUTTENHOWER; Alessio NACCARATI; Eva BUDINSKÁ a Nicola SEGATA. Pooled analysis of 3,741 stool metagenomes from 18 cohorts for cross-stage and strain-level reproducible microbial biomarkers of colorectal cancer. \textit{Nature Medicine}. Nature Research, 2025, roč.~31, č.~7, s.~2416-2429. ISSN~1078-8956. Dostupné z: https://doi.org/10.1038/s41591-025-03693-9.

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