Dbl2 interacts with helicases and an endonuclease to maintain
the integrity of repetitive regions

J 2025

Dbl2 interacts with helicases and an endonuclease to maintain the integrity of repetitive regions

BAKOSOVA, Anetta; Lubos CIPAK; Nina MAYEROVA; Kamil KROL; Zsigmond BENKO et al.

Základní údaje

Originální název

Dbl2 interacts with helicases and an endonuclease to maintain the integrity of repetitive regions

Autoři

Vydání

Scientific Reports, Berlin, NATURE RESEARCH, 2025, 2045-2322

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.900 v roce 2024

Označené pro přenos do RIV

Ano

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

Schizosaccharomyces pombe; DNA repair; Homologous recombination; Dbl2; Helicases

Štítky

14110513, 143160, podil, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 24. 2. 2026 07:21, Mgr. Tereza Miškechová

Anotace

V originále

Helicases and endonucleases play crucial roles in genome maintenance by unwinding or cleaving various forms of DNA and RNA structures in order to facilitate essential biological processes, such as DNA replication and recombination. Here, we identified fission yeast Dbl2 as a potential interactor of several complexes that exhibit either helicase or endonuclease activity, namely Fml1-MHF, SCFFbh1, Rqh1-Top3-Rmi1, and Mus81-Eme1. In vitro, Dbl2 binds to DNA, with a preference for branched molecules, such as D-loops, mobile Holliday junctions, and fork structures, making it a good candidate to play a central role in modulating the activity of helicases and endonucleases during replication and recombination repair. Previously, we showed that Dbl2 recruits Fbh1 to the ongoing homologous recombination sites, affecting the Rad51-nucleofilament. In this study, we determined that deleting dbl2 in an fbh1 Delta background did not increase sensitivity to DNA-damaging agents or the frequency of Tf2 ectopic recombination. Therefore, Dbl2 and Fbh1 might be involved in the same molecular pathway, maintaining genome integrity by hindering ectopic recombination at repetitive elements.

Návaznosti

GA23-05284S, projekt VaV

Název: Rozluštění vztahu mezi homologní rekombinací a umlčením genů

Investor: Grantová agentura ČR, Rozluštění vztahu mezi homologní rekombinací a umlčením genů

Přiložené soubory

s41598-025-08626-7.pdf

Citovat

BAKOSOVA, Anetta; Lubos CIPAK; Nina MAYEROVA; Kamil KROL; Zsigmond BENKO; Alexandra PITELOVA; Peter KOLESÁR; Dominika PIATROVA; Maria SMONDRKOVA; Anna MARESOVA; Lucia MOLNAROVA; Ingrid CIPAKOVA; Veronika ALTMANNOVÁ; Jana BELLOVA; Peter BARATH; Martin PREVOROVSKY; Jan PALEČEK; Lumír KREJČÍ; Juraj GREGAN; Adrianna SKONECZNA a Silvia BAGELOVA POLAKOVA. Dbl2 interacts with helicases and an endonuclease to maintain the integrity of repetitive regions. Scientific Reports. Berlin: NATURE RESEARCH, 2025, roč. 15, č. 1, s. 1-18. ISSN 2045-2322. Dostupné z: https://doi.org/10.1038/s41598-025-08626-7.

@article{2511399,
   author = {Bakosova, Anetta and Cipak, Lubos and Mayerova, Nina and Krol, Kamil and Benko, Zsigmond and Pitelova, Alexandra and Kolesár, Peter and Piatrova, Dominika and Smondrkova, Maria and Maresova, Anna and Molnarova, Lucia and Cipakova, Ingrid and Altmannová, Veronika and Bellova, Jana and Barath, Peter and Prevorovsky, Martin and Paleček, Jan and Krejčí, Lumír and Gregan, Juraj and Skoneczna, Adrianna and Bagelova Polakova, Silvia},
   article_location = {Berlin},
   article_number = {1},
   doi = {https://doi.org/10.1038/s41598-025-08626-7},
   keywords = {Schizosaccharomyces pombe; DNA repair; Homologous recombination; Dbl2; Helicases},
   language = {eng},
   issn = {2045-2322},
   journal = {Scientific Reports},
   title = {Dbl2 interacts with helicases and an endonuclease to maintain the integrity of repetitive regions},
   url = {https://www.nature.com/articles/s41598-025-08626-7},
   volume = {15},
   year = {2025}
}

TY  - JOUR
ID  - 2511399
AU  - Bakosova, Anetta - Cipak, Lubos - Mayerova, Nina - Krol, Kamil - Benko, Zsigmond - Pitelova, Alexandra - Kolesár, Peter - Piatrova, Dominika - Smondrkova, Maria - Maresova, Anna - Molnarova, Lucia - Cipakova, Ingrid - Altmannová, Veronika - Bellova, Jana - Barath, Peter - Prevorovsky, Martin - Paleček, Jan - Krejčí, Lumír - Gregan, Juraj - Skoneczna, Adrianna - Bagelova Polakova, Silvia
PY  - 2025
TI  - Dbl2 interacts with helicases and an endonuclease to maintain the integrity of repetitive regions
JF  - Scientific Reports
VL  - 15
IS  - 1
SP  - 1-18
EP  - 1-18
PB  - NATURE RESEARCH
SN  - 20452322
KW  - Schizosaccharomyces pombe
KW  - DNA repair
KW  - Homologous recombination
KW  - Dbl2
KW  - Helicases
UR  - https://www.nature.com/articles/s41598-025-08626-7
N2  - Helicases and endonucleases play crucial roles in genome maintenance by unwinding or cleaving various forms of DNA and RNA structures in order to facilitate essential biological processes, such as DNA replication and recombination. Here, we identified fission yeast Dbl2 as a potential interactor of several complexes that exhibit either helicase or endonuclease activity, namely Fml1-MHF, SCFFbh1, Rqh1-Top3-Rmi1, and Mus81-Eme1. In vitro, Dbl2 binds to DNA, with a preference for branched molecules, such as D-loops, mobile Holliday junctions, and fork structures, making it a good candidate to play a central role in modulating the activity of helicases and endonucleases during replication and recombination repair. Previously, we showed that Dbl2 recruits Fbh1 to the ongoing homologous recombination sites, affecting the Rad51-nucleofilament. In this study, we determined that deleting dbl2 in an fbh1 Delta background did not increase sensitivity to DNA-damaging agents or the frequency of Tf2 ectopic recombination. Therefore, Dbl2 and Fbh1 might be involved in the same molecular pathway, maintaining genome integrity by hindering ectopic recombination at repetitive elements.
ER  -

BAKOSOVA, Anetta; Lubos CIPAK; Nina MAYEROVA; Kamil KROL; Zsigmond BENKO; Alexandra PITELOVA; Peter KOLESÁR; Dominika PIATROVA; Maria SMONDRKOVA; Anna MARESOVA; Lucia MOLNAROVA; Ingrid CIPAKOVA; Veronika ALTMANNOVÁ; Jana BELLOVA; Peter BARATH; Martin PREVOROVSKY; Jan PALEČEK; Lumír KREJČÍ; Juraj GREGAN; Adrianna SKONECZNA a Silvia BAGELOVA POLAKOVA. Dbl2 interacts with helicases and an endonuclease to maintain the integrity of repetitive regions. \textit{Scientific Reports}. Berlin: NATURE RESEARCH, 2025, roč.~15, č.~1, s.~1-18. ISSN~2045-2322. Dostupné z: https://doi.org/10.1038/s41598-025-08626-7.

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