Dbl2 interacts with helicases and an endonuclease to maintain
the integrity of repetitive regions

J 2025

Dbl2 interacts with helicases and an endonuclease to maintain the integrity of repetitive regions

BAKOSOVA, Anetta; Lubos CIPAK; Nina MAYEROVA; Kamil KROL; Zsigmond BENKO et. al.

Basic information

Original name

Dbl2 interacts with helicases and an endonuclease to maintain the integrity of repetitive regions

Authors

BAKOSOVA, Anetta; Lubos CIPAK; Nina MAYEROVA; Kamil KROL; Zsigmond BENKO; Alexandra PITELOVA; Peter KOLESÁR (703 Slovakia, belonging to the institution); Dominika PIATROVA; Maria SMONDRKOVA; Anna MARESOVA; Lucia MOLNAROVA; Ingrid CIPAKOVA; Veronika ALTMANNOVÁ (203 Czech Republic, belonging to the institution); Jana BELLOVA; Peter BARATH; Martin PREVOROVSKY; Jan PALEČEK (203 Czech Republic, belonging to the institution); Lumír KREJČÍ (203 Czech Republic, belonging to the institution); Juraj GREGAN; Adrianna SKONECZNA and Silvia BAGELOVA POLAKOVA

Edition

Scientific Reports, Berlin, NATURE RESEARCH, 2025, 2045-2322

Other information

Language

English

Type of outcome

Article in a journal

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

Germany

Confidentiality degree

is not subject to a state or trade secret

References:

URL

Impact factor

Impact factor: 3.900 in 2024

Organization unit

Faculty of Medicine

DOI

https://doi.org/10.1038/s41598-025-08626-7

UT WoS

001522988900028

EID Scopus

2-s2.0-105009533605

Keywords in English

Schizosaccharomyces pombe; DNA repair; Homologous recombination; Dbl2; Helicases

Abstract

In the original language

Helicases and endonucleases play crucial roles in genome maintenance by unwinding or cleaving various forms of DNA and RNA structures in order to facilitate essential biological processes, such as DNA replication and recombination. Here, we identified fission yeast Dbl2 as a potential interactor of several complexes that exhibit either helicase or endonuclease activity, namely Fml1-MHF, SCFFbh1, Rqh1-Top3-Rmi1, and Mus81-Eme1. In vitro, Dbl2 binds to DNA, with a preference for branched molecules, such as D-loops, mobile Holliday junctions, and fork structures, making it a good candidate to play a central role in modulating the activity of helicases and endonucleases during replication and recombination repair. Previously, we showed that Dbl2 recruits Fbh1 to the ongoing homologous recombination sites, affecting the Rad51-nucleofilament. In this study, we determined that deleting dbl2 in an fbh1 Delta background did not increase sensitivity to DNA-damaging agents or the frequency of Tf2 ectopic recombination. Therefore, Dbl2 and Fbh1 might be involved in the same molecular pathway, maintaining genome integrity by hindering ectopic recombination at repetitive elements.

Links

GA23-05284S, research and development project

Name: Rozluštění vztahu mezi homologní rekombinací a umlčením genů

Investor: Czech Science Foundation, Deciphering the relationship between homolognous recombination and gene silencing

Cite

BAKOSOVA, Anetta; Lubos CIPAK; Nina MAYEROVA; Kamil KROL; Zsigmond BENKO; Alexandra PITELOVA; Peter KOLESÁR; Dominika PIATROVA; Maria SMONDRKOVA; Anna MARESOVA; Lucia MOLNAROVA; Ingrid CIPAKOVA; Veronika ALTMANNOVÁ; Jana BELLOVA; Peter BARATH; Martin PREVOROVSKY; Jan PALEČEK; Lumír KREJČÍ; Juraj GREGAN; Adrianna SKONECZNA and Silvia BAGELOVA POLAKOVA. Dbl2 interacts with helicases and an endonuclease to maintain the integrity of repetitive regions. Scientific Reports. Berlin: NATURE RESEARCH, 2025, vol. 15, No 1, p. 1-18. ISSN 2045-2322. Available from: https://doi.org/10.1038/s41598-025-08626-7.

@article{2511399,
   author = {Bakosova, Anetta and Cipak, Lubos and Mayerova, Nina and Krol, Kamil and Benko, Zsigmond and Pitelova, Alexandra and Kolesár, Peter and Piatrova, Dominika and Smondrkova, Maria and Maresova, Anna and Molnarova, Lucia and Cipakova, Ingrid and Altmannová, Veronika and Bellova, Jana and Barath, Peter and Prevorovsky, Martin and Paleček, Jan and Krejčí, Lumír and Gregan, Juraj and Skoneczna, Adrianna and Bagelova Polakova, Silvia},
   article_location = {Berlin},
   article_number = {1},
   doi = {https://doi.org/10.1038/s41598-025-08626-7},
   keywords = {Schizosaccharomyces pombe; DNA repair; Homologous recombination; Dbl2; Helicases},
   language = {eng},
   issn = {2045-2322},
   journal = {Scientific Reports},
   title = {Dbl2 interacts with helicases and an endonuclease to maintain the integrity of repetitive regions},
   url = {https://www.nature.com/articles/s41598-025-08626-7},
   volume = {15},
   year = {2025}
}

TY  - JOUR
ID  - 2511399
AU  - Bakosova, Anetta - Cipak, Lubos - Mayerova, Nina - Krol, Kamil - Benko, Zsigmond - Pitelova, Alexandra - Kolesár, Peter - Piatrova, Dominika - Smondrkova, Maria - Maresova, Anna - Molnarova, Lucia - Cipakova, Ingrid - Altmannová, Veronika - Bellova, Jana - Barath, Peter - Prevorovsky, Martin - Paleček, Jan - Krejčí, Lumír - Gregan, Juraj - Skoneczna, Adrianna - Bagelova Polakova, Silvia
PY  - 2025
TI  - Dbl2 interacts with helicases and an endonuclease to maintain the integrity of repetitive regions
JF  - Scientific Reports
VL  - 15
IS  - 1
SP  - 1-18
EP  - 1-18
PB  - NATURE RESEARCH
SN  - 20452322
KW  - Schizosaccharomyces pombe
KW  - DNA repair
KW  - Homologous recombination
KW  - Dbl2
KW  - Helicases
UR  - https://www.nature.com/articles/s41598-025-08626-7
N2  - Helicases and endonucleases play crucial roles in genome maintenance by unwinding or cleaving various forms of DNA and RNA structures in order to facilitate essential biological processes, such as DNA replication and recombination. Here, we identified fission yeast Dbl2 as a potential interactor of several complexes that exhibit either helicase or endonuclease activity, namely Fml1-MHF, SCFFbh1, Rqh1-Top3-Rmi1, and Mus81-Eme1. In vitro, Dbl2 binds to DNA, with a preference for branched molecules, such as D-loops, mobile Holliday junctions, and fork structures, making it a good candidate to play a central role in modulating the activity of helicases and endonucleases during replication and recombination repair. Previously, we showed that Dbl2 recruits Fbh1 to the ongoing homologous recombination sites, affecting the Rad51-nucleofilament. In this study, we determined that deleting dbl2 in an fbh1 Delta background did not increase sensitivity to DNA-damaging agents or the frequency of Tf2 ectopic recombination. Therefore, Dbl2 and Fbh1 might be involved in the same molecular pathway, maintaining genome integrity by hindering ectopic recombination at repetitive elements.
ER  -

BAKOSOVA, Anetta; Lubos CIPAK; Nina MAYEROVA; Kamil KROL; Zsigmond BENKO; Alexandra PITELOVA; Peter KOLESÁR; Dominika PIATROVA; Maria SMONDRKOVA; Anna MARESOVA; Lucia MOLNAROVA; Ingrid CIPAKOVA; Veronika ALTMANNOVÁ; Jana BELLOVA; Peter BARATH; Martin PREVOROVSKY; Jan PALEČEK; Lumír KREJČÍ; Juraj GREGAN; Adrianna SKONECZNA and Silvia BAGELOVA POLAKOVA. Dbl2 interacts with helicases and an endonuclease to maintain the integrity of repetitive regions. \textit{Scientific Reports}. Berlin: NATURE RESEARCH, 2025, vol.~15, No~1, p.~1-18. ISSN~2045-2322. Available from: https://doi.org/10.1038/s41598-025-08626-7.

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