Membrane Transport of Root-Borne trans-Zeatin Riboside
Maintains the Cytokinin Homeostasis in Shoots

J 2025

Membrane Transport of Root-Borne trans-Zeatin Riboside Maintains the Cytokinin Homeostasis in Shoots

NEDVĚD, Daniel; Martin HUDEČEK; Petr KLÍMA; Jozef LACEK; Karel MÜLLER et al.

Základní údaje

Originální název

Membrane Transport of Root-Borne trans-Zeatin Riboside Maintains the Cytokinin Homeostasis in Shoots

Autoři

NEDVĚD, Daniel; Martin HUDEČEK; Petr KLÍMA; Jozef LACEK; Karel MÜLLER; Anna KUCHAŘOVÁ; Petr HOŠEK; Ján ŠMERINGAI; Markéta PERNISOVÁ; Václav MOTYKA; Ondřej PLÍHAL a Klára HOYEROVÁ

Vydání

JOURNAL OF EXPERIMENTAL BOTANY, OXFORD UNIV PRESS, 2025, 0022-0957

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL, URL

Impakt faktor

Impact factor: 5.700 v roce 2024

Označené pro přenos do RIV

Ano

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

cytokinin; cytokinin transport; cytokinin riboside; equilibrative nucleoside transporter; ENT3; membrane transport

Štítky

143160, CF PLANT, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 18. 3. 2026 18:05, Mgr. Markéta Pernisová, Ph.D.

Anotace

V originále

Ribosylated forms of plant hormones cytokinins (CKs) are the dominant CK species translocated at long distances. The irreplaceable role of root-to-shoot translocated trans-zeatin riboside (tZR) in the mediation of shoot development implies the existence of a yet uncharacterized CK riboside-specific membrane transport system. In this work, we report significant differences in the kinetics of the membrane transport of CK nucleobases and ribosides and the overall affinity of membrane-bound carriers towards the two CK forms. We further characterize the membrane transport of CK nucleobases and ribosides mediated by AtENT3 (EQULIBRATIVE NUCLEOSIDE TRANSPORTER 3) in tobacco BY-2 cells. Combining experimental data with computational modelling, we show that residues Tyr61 and Asp129, which are conserved among plant ENTs but not among ENTs from other species, are necessary for CK binding and that their mutation abolishes the ability of AtENT3 to transport CKs. Lastly, we show that changes in AtENT3 have different effects on the concentrations of tZR throughout Arabidopsis thaliana plants and on the overall CK concentrations in roots, implying that AtENT3 participates both on the long- and short-distance transport of CKs.

Návaznosti

EH22_008/0004581, projekt VaV

Název: Nové poznatky pro plodiny nové generace

Přiložené soubory

2518782.pdf

eraf369.pdf

Citovat

NEDVĚD, Daniel; Martin HUDEČEK; Petr KLÍMA; Jozef LACEK; Karel MÜLLER; Anna KUCHAŘOVÁ; Petr HOŠEK; Ján ŠMERINGAI; Markéta PERNISOVÁ; Václav MOTYKA; Ondřej PLÍHAL a Klára HOYEROVÁ. Membrane Transport of Root-Borne trans-Zeatin Riboside Maintains the Cytokinin Homeostasis in Shoots. JOURNAL OF EXPERIMENTAL BOTANY. OXFORD UNIV PRESS, 2025, roč. 76, č. 22, s. 6723-6740. ISSN 0022-0957. Dostupné z: https://doi.org/10.1093/jxb/eraf369.

@article{2518782,
   author = {Nedvěd, Daniel and Hudeček, Martin and Klíma, Petr and Lacek, Jozef and Müller, Karel and Kuchařová, Anna and Hošek, Petr and Šmeringai, Ján and Pernisová, Markéta and Motyka, Václav and Plíhal, Ondřej and Hoyerová, Klára},
   article_number = {22},
   doi = {https://doi.org/10.1093/jxb/eraf369},
   keywords = {cytokinin; cytokinin transport; cytokinin riboside; equilibrative nucleoside transporter; ENT3; membrane transport},
   language = {eng},
   issn = {0022-0957},
   journal = {JOURNAL OF EXPERIMENTAL BOTANY},
   title = {Membrane Transport of Root-Borne trans-Zeatin Riboside Maintains the Cytokinin Homeostasis in Shoots},
   url = {https://academic.oup.com/jxb/advance-article/doi/10.1093/jxb/eraf369/8240828},
   volume = {76},
   year = {2025}
}

TY  - JOUR
ID  - 2518782
AU  - Nedvěd, Daniel - Hudeček, Martin - Klíma, Petr - Lacek, Jozef - Müller, Karel - Kuchařová, Anna - Hošek, Petr - Šmeringai, Ján - Pernisová, Markéta - Motyka, Václav - Plíhal, Ondřej - Hoyerová, Klára
PY  - 2025
TI  - Membrane Transport of Root-Borne trans-Zeatin Riboside Maintains the Cytokinin Homeostasis in Shoots
JF  - JOURNAL OF EXPERIMENTAL BOTANY
VL  - 76
IS  - 22
SP  - 6723-6740
EP  - 6723-6740
PB  - OXFORD UNIV PRESS
SN  - 00220957
KW  - cytokinin
KW  - cytokinin transport
KW  - cytokinin riboside
KW  - equilibrative nucleoside transporter
KW  - ENT3
KW  - membrane transport
UR  - https://academic.oup.com/jxb/advance-article/doi/10.1093/jxb/eraf369/8240828
N2  - Ribosylated forms of plant hormones cytokinins (CKs) are the dominant CK species translocated at long distances. The irreplaceable role of root-to-shoot translocated trans-zeatin riboside (tZR) in the mediation of shoot development implies the existence of a yet uncharacterized CK riboside-specific membrane transport system. In this work, we report significant differences in the kinetics of the membrane transport of CK nucleobases and ribosides and the overall affinity of membrane-bound carriers towards the two CK forms. We further characterize the membrane transport of CK nucleobases and ribosides mediated by AtENT3 (EQULIBRATIVE NUCLEOSIDE TRANSPORTER 3) in tobacco BY-2 cells. Combining experimental data with computational modelling, we show that residues Tyr61 and Asp129, which are conserved among plant ENTs but not among ENTs from other species, are necessary for CK binding and that their mutation abolishes the ability of AtENT3 to transport CKs. Lastly, we show that changes in AtENT3 have different effects on the concentrations of tZR throughout Arabidopsis thaliana plants and on the overall CK concentrations in roots, implying that AtENT3 participates both on the long- and short-distance transport of CKs.
ER  -

NEDVĚD, Daniel; Martin HUDEČEK; Petr KLÍMA; Jozef LACEK; Karel MÜLLER; Anna KUCHAŘOVÁ; Petr HOŠEK; Ján ŠMERINGAI; Markéta PERNISOVÁ; Václav MOTYKA; Ondřej PLÍHAL a Klára HOYEROVÁ. Membrane Transport of Root-Borne trans-Zeatin Riboside Maintains the Cytokinin Homeostasis in Shoots. \textit{JOURNAL OF EXPERIMENTAL BOTANY}. OXFORD UNIV PRESS, 2025, roč.~76, č.~22, s.~6723-6740. ISSN~0022-0957. Dostupné z: https://doi.org/10.1093/jxb/eraf369.

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